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Analysis of the saliva metabolic signature in patients with primary Sjögren’s syndrome
BACKGROUND: The saliva metabolome has been applied to explore disease biomarkers. In this study we characterized the metabolic profile of primary Sjögren’s syndrome (pSS) patients and explored metabolomic biomarkers. METHODS: This work presents a liquid chromatography-mass spectrometry-based metabol...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9162338/ https://www.ncbi.nlm.nih.gov/pubmed/35653354 http://dx.doi.org/10.1371/journal.pone.0269275 |
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author | Li, Zhen Mu, Yue Guo, Chunlan You, Xin Liu, Xiaoyan Li, Qian Sun, Wei |
author_facet | Li, Zhen Mu, Yue Guo, Chunlan You, Xin Liu, Xiaoyan Li, Qian Sun, Wei |
author_sort | Li, Zhen |
collection | PubMed |
description | BACKGROUND: The saliva metabolome has been applied to explore disease biomarkers. In this study we characterized the metabolic profile of primary Sjögren’s syndrome (pSS) patients and explored metabolomic biomarkers. METHODS: This work presents a liquid chromatography-mass spectrometry-based metabolomic study of the saliva of 32 patients with pSS and 38 age- and sex-matched healthy adults. Potential pSS saliva metabolite biomarkers were explored using test group saliva samples (20 patients with pSS vs. 25 healthy adults) and were then verified by a cross-validation group (12 patients with pSS vs. 13 healthy adults). RESULTS: Metabolic pathways, including tryptophan metabolism, tyrosine metabolism, carbon fixation, and aspartate and asparagine metabolism, were found to be significantly regulated and related to inflammatory injury, neurological cognitive impairment and the immune response. Phenylalanyl-alanine was discovered to have good predictive ability for pSS, with an area under the curve (AUC) of 0.87 in the testing group (validation group: AUC = 0.75). CONCLUSION: Our study shows that salivary metabolomics is a useful strategy for differential analysis and biomarker discovery in pSS. |
format | Online Article Text |
id | pubmed-9162338 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-91623382022-06-03 Analysis of the saliva metabolic signature in patients with primary Sjögren’s syndrome Li, Zhen Mu, Yue Guo, Chunlan You, Xin Liu, Xiaoyan Li, Qian Sun, Wei PLoS One Research Article BACKGROUND: The saliva metabolome has been applied to explore disease biomarkers. In this study we characterized the metabolic profile of primary Sjögren’s syndrome (pSS) patients and explored metabolomic biomarkers. METHODS: This work presents a liquid chromatography-mass spectrometry-based metabolomic study of the saliva of 32 patients with pSS and 38 age- and sex-matched healthy adults. Potential pSS saliva metabolite biomarkers were explored using test group saliva samples (20 patients with pSS vs. 25 healthy adults) and were then verified by a cross-validation group (12 patients with pSS vs. 13 healthy adults). RESULTS: Metabolic pathways, including tryptophan metabolism, tyrosine metabolism, carbon fixation, and aspartate and asparagine metabolism, were found to be significantly regulated and related to inflammatory injury, neurological cognitive impairment and the immune response. Phenylalanyl-alanine was discovered to have good predictive ability for pSS, with an area under the curve (AUC) of 0.87 in the testing group (validation group: AUC = 0.75). CONCLUSION: Our study shows that salivary metabolomics is a useful strategy for differential analysis and biomarker discovery in pSS. Public Library of Science 2022-06-02 /pmc/articles/PMC9162338/ /pubmed/35653354 http://dx.doi.org/10.1371/journal.pone.0269275 Text en © 2022 Li et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Li, Zhen Mu, Yue Guo, Chunlan You, Xin Liu, Xiaoyan Li, Qian Sun, Wei Analysis of the saliva metabolic signature in patients with primary Sjögren’s syndrome |
title | Analysis of the saliva metabolic signature in patients with primary Sjögren’s syndrome |
title_full | Analysis of the saliva metabolic signature in patients with primary Sjögren’s syndrome |
title_fullStr | Analysis of the saliva metabolic signature in patients with primary Sjögren’s syndrome |
title_full_unstemmed | Analysis of the saliva metabolic signature in patients with primary Sjögren’s syndrome |
title_short | Analysis of the saliva metabolic signature in patients with primary Sjögren’s syndrome |
title_sort | analysis of the saliva metabolic signature in patients with primary sjögren’s syndrome |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9162338/ https://www.ncbi.nlm.nih.gov/pubmed/35653354 http://dx.doi.org/10.1371/journal.pone.0269275 |
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