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No-nonsense: insights into the functional interplay of nonsense-mediated mRNA decay factors

Nonsense-mediated messenger RNA decay (NMD) represents one of the main surveillance pathways used by eukaryotic cells to control the quality and abundance of mRNAs and to degrade viral RNA. NMD recognises mRNAs with a premature termination codon (PTC) and targets them to decay. Markers for a mRNA wi...

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Autores principales: Mailliot, Justine, Vivoli-Vega, Mirella, Schaffitzel, Christiane
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9162471/
https://www.ncbi.nlm.nih.gov/pubmed/35551602
http://dx.doi.org/10.1042/BCJ20210556
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author Mailliot, Justine
Vivoli-Vega, Mirella
Schaffitzel, Christiane
author_facet Mailliot, Justine
Vivoli-Vega, Mirella
Schaffitzel, Christiane
author_sort Mailliot, Justine
collection PubMed
description Nonsense-mediated messenger RNA decay (NMD) represents one of the main surveillance pathways used by eukaryotic cells to control the quality and abundance of mRNAs and to degrade viral RNA. NMD recognises mRNAs with a premature termination codon (PTC) and targets them to decay. Markers for a mRNA with a PTC, and thus NMD, are a long a 3′-untranslated region and the presence of an exon-junction complex (EJC) downstream of the stop codon. Here, we review our structural understanding of mammalian NMD factors and their functional interplay leading to a branched network of different interconnected but specialised mRNA decay pathways. We discuss recent insights into the potential impact of EJC composition on NMD pathway choice. We highlight the coexistence and function of different isoforms of up-frameshift protein 1 (UPF1) with an emphasis of their role at the endoplasmic reticulum and during stress, and the role of the paralogs UPF3B and UPF3A, underscoring that gene regulation by mammalian NMD is tightly controlled and context-dependent being conditional on developmental stage, tissue and cell types.
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spelling pubmed-91624712022-06-07 No-nonsense: insights into the functional interplay of nonsense-mediated mRNA decay factors Mailliot, Justine Vivoli-Vega, Mirella Schaffitzel, Christiane Biochem J Gene Expression & Regulation Nonsense-mediated messenger RNA decay (NMD) represents one of the main surveillance pathways used by eukaryotic cells to control the quality and abundance of mRNAs and to degrade viral RNA. NMD recognises mRNAs with a premature termination codon (PTC) and targets them to decay. Markers for a mRNA with a PTC, and thus NMD, are a long a 3′-untranslated region and the presence of an exon-junction complex (EJC) downstream of the stop codon. Here, we review our structural understanding of mammalian NMD factors and their functional interplay leading to a branched network of different interconnected but specialised mRNA decay pathways. We discuss recent insights into the potential impact of EJC composition on NMD pathway choice. We highlight the coexistence and function of different isoforms of up-frameshift protein 1 (UPF1) with an emphasis of their role at the endoplasmic reticulum and during stress, and the role of the paralogs UPF3B and UPF3A, underscoring that gene regulation by mammalian NMD is tightly controlled and context-dependent being conditional on developmental stage, tissue and cell types. Portland Press Ltd. 2022-05-12 /pmc/articles/PMC9162471/ /pubmed/35551602 http://dx.doi.org/10.1042/BCJ20210556 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Gene Expression & Regulation
Mailliot, Justine
Vivoli-Vega, Mirella
Schaffitzel, Christiane
No-nonsense: insights into the functional interplay of nonsense-mediated mRNA decay factors
title No-nonsense: insights into the functional interplay of nonsense-mediated mRNA decay factors
title_full No-nonsense: insights into the functional interplay of nonsense-mediated mRNA decay factors
title_fullStr No-nonsense: insights into the functional interplay of nonsense-mediated mRNA decay factors
title_full_unstemmed No-nonsense: insights into the functional interplay of nonsense-mediated mRNA decay factors
title_short No-nonsense: insights into the functional interplay of nonsense-mediated mRNA decay factors
title_sort no-nonsense: insights into the functional interplay of nonsense-mediated mrna decay factors
topic Gene Expression & Regulation
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9162471/
https://www.ncbi.nlm.nih.gov/pubmed/35551602
http://dx.doi.org/10.1042/BCJ20210556
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