Cargando…
No-nonsense: insights into the functional interplay of nonsense-mediated mRNA decay factors
Nonsense-mediated messenger RNA decay (NMD) represents one of the main surveillance pathways used by eukaryotic cells to control the quality and abundance of mRNAs and to degrade viral RNA. NMD recognises mRNAs with a premature termination codon (PTC) and targets them to decay. Markers for a mRNA wi...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9162471/ https://www.ncbi.nlm.nih.gov/pubmed/35551602 http://dx.doi.org/10.1042/BCJ20210556 |
_version_ | 1784719711733284864 |
---|---|
author | Mailliot, Justine Vivoli-Vega, Mirella Schaffitzel, Christiane |
author_facet | Mailliot, Justine Vivoli-Vega, Mirella Schaffitzel, Christiane |
author_sort | Mailliot, Justine |
collection | PubMed |
description | Nonsense-mediated messenger RNA decay (NMD) represents one of the main surveillance pathways used by eukaryotic cells to control the quality and abundance of mRNAs and to degrade viral RNA. NMD recognises mRNAs with a premature termination codon (PTC) and targets them to decay. Markers for a mRNA with a PTC, and thus NMD, are a long a 3′-untranslated region and the presence of an exon-junction complex (EJC) downstream of the stop codon. Here, we review our structural understanding of mammalian NMD factors and their functional interplay leading to a branched network of different interconnected but specialised mRNA decay pathways. We discuss recent insights into the potential impact of EJC composition on NMD pathway choice. We highlight the coexistence and function of different isoforms of up-frameshift protein 1 (UPF1) with an emphasis of their role at the endoplasmic reticulum and during stress, and the role of the paralogs UPF3B and UPF3A, underscoring that gene regulation by mammalian NMD is tightly controlled and context-dependent being conditional on developmental stage, tissue and cell types. |
format | Online Article Text |
id | pubmed-9162471 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-91624712022-06-07 No-nonsense: insights into the functional interplay of nonsense-mediated mRNA decay factors Mailliot, Justine Vivoli-Vega, Mirella Schaffitzel, Christiane Biochem J Gene Expression & Regulation Nonsense-mediated messenger RNA decay (NMD) represents one of the main surveillance pathways used by eukaryotic cells to control the quality and abundance of mRNAs and to degrade viral RNA. NMD recognises mRNAs with a premature termination codon (PTC) and targets them to decay. Markers for a mRNA with a PTC, and thus NMD, are a long a 3′-untranslated region and the presence of an exon-junction complex (EJC) downstream of the stop codon. Here, we review our structural understanding of mammalian NMD factors and their functional interplay leading to a branched network of different interconnected but specialised mRNA decay pathways. We discuss recent insights into the potential impact of EJC composition on NMD pathway choice. We highlight the coexistence and function of different isoforms of up-frameshift protein 1 (UPF1) with an emphasis of their role at the endoplasmic reticulum and during stress, and the role of the paralogs UPF3B and UPF3A, underscoring that gene regulation by mammalian NMD is tightly controlled and context-dependent being conditional on developmental stage, tissue and cell types. Portland Press Ltd. 2022-05-12 /pmc/articles/PMC9162471/ /pubmed/35551602 http://dx.doi.org/10.1042/BCJ20210556 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Gene Expression & Regulation Mailliot, Justine Vivoli-Vega, Mirella Schaffitzel, Christiane No-nonsense: insights into the functional interplay of nonsense-mediated mRNA decay factors |
title | No-nonsense: insights into the functional interplay of nonsense-mediated mRNA decay factors |
title_full | No-nonsense: insights into the functional interplay of nonsense-mediated mRNA decay factors |
title_fullStr | No-nonsense: insights into the functional interplay of nonsense-mediated mRNA decay factors |
title_full_unstemmed | No-nonsense: insights into the functional interplay of nonsense-mediated mRNA decay factors |
title_short | No-nonsense: insights into the functional interplay of nonsense-mediated mRNA decay factors |
title_sort | no-nonsense: insights into the functional interplay of nonsense-mediated mrna decay factors |
topic | Gene Expression & Regulation |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9162471/ https://www.ncbi.nlm.nih.gov/pubmed/35551602 http://dx.doi.org/10.1042/BCJ20210556 |
work_keys_str_mv | AT mailliotjustine nononsenseinsightsintothefunctionalinterplayofnonsensemediatedmrnadecayfactors AT vivolivegamirella nononsenseinsightsintothefunctionalinterplayofnonsensemediatedmrnadecayfactors AT schaffitzelchristiane nononsenseinsightsintothefunctionalinterplayofnonsensemediatedmrnadecayfactors |