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Molecular detection of porcine parvovirus 1–associated reproductive failure in southern India
This study used 56 aborted and stillborn fetuses from organized swine farms in Tamil Nadu and Kerala, southern states of India. All samples were screened by using a PCR assay that targets the NS1 gene for PPV. Furthermore, the PCR positive samples were subjected to amplification of the VP2 gene of P...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Netherlands
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9162486/ https://www.ncbi.nlm.nih.gov/pubmed/35655031 http://dx.doi.org/10.1007/s11250-022-03194-8 |
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author | Parthiban, S. Sowndhraya, R. K. V. Raja, P. Parthiban, M. Ramesh, A. Raj, G. Dhinakar Senthilkumar, K. Balasubramanyam, D. Hemalatha, S. Bharathi, R. Ravishankar, Chintu Parveen, S. Thahira |
author_facet | Parthiban, S. Sowndhraya, R. K. V. Raja, P. Parthiban, M. Ramesh, A. Raj, G. Dhinakar Senthilkumar, K. Balasubramanyam, D. Hemalatha, S. Bharathi, R. Ravishankar, Chintu Parveen, S. Thahira |
author_sort | Parthiban, S. |
collection | PubMed |
description | This study used 56 aborted and stillborn fetuses from organized swine farms in Tamil Nadu and Kerala, southern states of India. All samples were screened by using a PCR assay that targets the NS1 gene for PPV. Furthermore, the PCR positive samples were subjected to amplification of the VP2 gene of PPV1 with designed primers and sequenced for further study. The PCR screening of 56 samples found that 14.3% (n = 8) were positive for PPV genome. According to VP2 gene–based PCR for PPV1, 897 bp specific amplicons were detected in all eight of the samples. Two of the eight positive samples (L17 and T5) were sequenced and annotated randomly. The BLAST analysis of contig sequence INDTNCHN-T5 revealed 100% sequence homology with Chinese PPV1genome, whereas sequence from INDTNCHN-L17 revealed 99.43% sequence homology with Spain, Chinese, and German. PPV1 sequences and both the sequences INDTNCHN-T5 and INDTNCHN-L17 were submitted to the GenBank under the accession numbers MW822566 and MW822567 respectively. A phylogenetic analysis of the sequences in this study revealed specific grouping along with PPV1 strains in cluster E. Amino acid analysis of both isolated sequences in addition to the reference sequence from PPV1 showed variations in position 215 (I to T) in both the isolates, variation at position 228 (Q to E) in T5 isolate and variations at position 59 (L to M) and 314 (K to E) in L17 isolate. This study represents the first report of PPV1 cluster E in Tamil Nadu, southern India. |
format | Online Article Text |
id | pubmed-9162486 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Springer Netherlands |
record_format | MEDLINE/PubMed |
spelling | pubmed-91624862022-06-04 Molecular detection of porcine parvovirus 1–associated reproductive failure in southern India Parthiban, S. Sowndhraya, R. K. V. Raja, P. Parthiban, M. Ramesh, A. Raj, G. Dhinakar Senthilkumar, K. Balasubramanyam, D. Hemalatha, S. Bharathi, R. Ravishankar, Chintu Parveen, S. Thahira Trop Anim Health Prod Regular Articles This study used 56 aborted and stillborn fetuses from organized swine farms in Tamil Nadu and Kerala, southern states of India. All samples were screened by using a PCR assay that targets the NS1 gene for PPV. Furthermore, the PCR positive samples were subjected to amplification of the VP2 gene of PPV1 with designed primers and sequenced for further study. The PCR screening of 56 samples found that 14.3% (n = 8) were positive for PPV genome. According to VP2 gene–based PCR for PPV1, 897 bp specific amplicons were detected in all eight of the samples. Two of the eight positive samples (L17 and T5) were sequenced and annotated randomly. The BLAST analysis of contig sequence INDTNCHN-T5 revealed 100% sequence homology with Chinese PPV1genome, whereas sequence from INDTNCHN-L17 revealed 99.43% sequence homology with Spain, Chinese, and German. PPV1 sequences and both the sequences INDTNCHN-T5 and INDTNCHN-L17 were submitted to the GenBank under the accession numbers MW822566 and MW822567 respectively. A phylogenetic analysis of the sequences in this study revealed specific grouping along with PPV1 strains in cluster E. Amino acid analysis of both isolated sequences in addition to the reference sequence from PPV1 showed variations in position 215 (I to T) in both the isolates, variation at position 228 (Q to E) in T5 isolate and variations at position 59 (L to M) and 314 (K to E) in L17 isolate. This study represents the first report of PPV1 cluster E in Tamil Nadu, southern India. Springer Netherlands 2022-06-02 2022 /pmc/articles/PMC9162486/ /pubmed/35655031 http://dx.doi.org/10.1007/s11250-022-03194-8 Text en © The Author(s), under exclusive licence to Springer Nature B.V. 2022 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Regular Articles Parthiban, S. Sowndhraya, R. K. V. Raja, P. Parthiban, M. Ramesh, A. Raj, G. Dhinakar Senthilkumar, K. Balasubramanyam, D. Hemalatha, S. Bharathi, R. Ravishankar, Chintu Parveen, S. Thahira Molecular detection of porcine parvovirus 1–associated reproductive failure in southern India |
title | Molecular detection of porcine parvovirus 1–associated reproductive failure in southern India |
title_full | Molecular detection of porcine parvovirus 1–associated reproductive failure in southern India |
title_fullStr | Molecular detection of porcine parvovirus 1–associated reproductive failure in southern India |
title_full_unstemmed | Molecular detection of porcine parvovirus 1–associated reproductive failure in southern India |
title_short | Molecular detection of porcine parvovirus 1–associated reproductive failure in southern India |
title_sort | molecular detection of porcine parvovirus 1–associated reproductive failure in southern india |
topic | Regular Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9162486/ https://www.ncbi.nlm.nih.gov/pubmed/35655031 http://dx.doi.org/10.1007/s11250-022-03194-8 |
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