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Cassava shrunken-2 homolog MeAPL3 determines storage root starch and dry matter content and modulates storage root postharvest physiological deterioration
KEY MESSAGE: Among the five cassava isoforms (MeAPL1–MeAPL5), MeAPL3 is responsible for determining storage root starch content. Degree of storage root postharvest physiological deterioration (PPD) is directly correlated with starch content. ABSTRACT: AGPase is heterotetramer composed of two small a...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Netherlands
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9163024/ https://www.ncbi.nlm.nih.gov/pubmed/32270429 http://dx.doi.org/10.1007/s11103-020-00995-z |
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author | Beyene, Getu Chauhan, Raj Deepika Gehan, Jackson Siritunga, Dimuth Taylor, Nigel |
author_facet | Beyene, Getu Chauhan, Raj Deepika Gehan, Jackson Siritunga, Dimuth Taylor, Nigel |
author_sort | Beyene, Getu |
collection | PubMed |
description | KEY MESSAGE: Among the five cassava isoforms (MeAPL1–MeAPL5), MeAPL3 is responsible for determining storage root starch content. Degree of storage root postharvest physiological deterioration (PPD) is directly correlated with starch content. ABSTRACT: AGPase is heterotetramer composed of two small and two large subunits each coded by small gene families in higher plants. Studies in cassava (Manihot esculenta) identified and characterized five isoforms of Manihot esculenta ADP-glucose pyrophosphorylase large subunit (MeAPL1–MeAPL5) and employed virus induced gene silencing (VIGS) to show that MeAPL3 is the key isoform responsible for starch and dry matter accumulation in cassava storage roots. Silencing of MeAPL3 in cassava through stable transgenic lines resulted in plants displaying significant reduction in storage root starch and dry matter content (DMC) and induced a distinct phenotype associated with increased petiole/stem angle, resulting in a droopy leaf phenotype. Plants with reduced starch and DMC also displayed significantly reduced or no postharvest physiological deterioration (PPD) compared to controls and lines with high DMC and starch content. This provides strong evidence for direct relationships between starch/dry matter content and its role in PPD and canopy architecture traits in cassava. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s11103-020-00995-z) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-9163024 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Springer Netherlands |
record_format | MEDLINE/PubMed |
spelling | pubmed-91630242022-06-05 Cassava shrunken-2 homolog MeAPL3 determines storage root starch and dry matter content and modulates storage root postharvest physiological deterioration Beyene, Getu Chauhan, Raj Deepika Gehan, Jackson Siritunga, Dimuth Taylor, Nigel Plant Mol Biol Article KEY MESSAGE: Among the five cassava isoforms (MeAPL1–MeAPL5), MeAPL3 is responsible for determining storage root starch content. Degree of storage root postharvest physiological deterioration (PPD) is directly correlated with starch content. ABSTRACT: AGPase is heterotetramer composed of two small and two large subunits each coded by small gene families in higher plants. Studies in cassava (Manihot esculenta) identified and characterized five isoforms of Manihot esculenta ADP-glucose pyrophosphorylase large subunit (MeAPL1–MeAPL5) and employed virus induced gene silencing (VIGS) to show that MeAPL3 is the key isoform responsible for starch and dry matter accumulation in cassava storage roots. Silencing of MeAPL3 in cassava through stable transgenic lines resulted in plants displaying significant reduction in storage root starch and dry matter content (DMC) and induced a distinct phenotype associated with increased petiole/stem angle, resulting in a droopy leaf phenotype. Plants with reduced starch and DMC also displayed significantly reduced or no postharvest physiological deterioration (PPD) compared to controls and lines with high DMC and starch content. This provides strong evidence for direct relationships between starch/dry matter content and its role in PPD and canopy architecture traits in cassava. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s11103-020-00995-z) contains supplementary material, which is available to authorized users. Springer Netherlands 2020-10-06 2022 /pmc/articles/PMC9163024/ /pubmed/32270429 http://dx.doi.org/10.1007/s11103-020-00995-z Text en © The Author(s) 2020 https://creativecommons.org/licenses/by/4.0/ Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Beyene, Getu Chauhan, Raj Deepika Gehan, Jackson Siritunga, Dimuth Taylor, Nigel Cassava shrunken-2 homolog MeAPL3 determines storage root starch and dry matter content and modulates storage root postharvest physiological deterioration |
title | Cassava shrunken-2 homolog MeAPL3 determines storage root starch and dry matter content and modulates storage root postharvest physiological deterioration |
title_full | Cassava shrunken-2 homolog MeAPL3 determines storage root starch and dry matter content and modulates storage root postharvest physiological deterioration |
title_fullStr | Cassava shrunken-2 homolog MeAPL3 determines storage root starch and dry matter content and modulates storage root postharvest physiological deterioration |
title_full_unstemmed | Cassava shrunken-2 homolog MeAPL3 determines storage root starch and dry matter content and modulates storage root postharvest physiological deterioration |
title_short | Cassava shrunken-2 homolog MeAPL3 determines storage root starch and dry matter content and modulates storage root postharvest physiological deterioration |
title_sort | cassava shrunken-2 homolog meapl3 determines storage root starch and dry matter content and modulates storage root postharvest physiological deterioration |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9163024/ https://www.ncbi.nlm.nih.gov/pubmed/32270429 http://dx.doi.org/10.1007/s11103-020-00995-z |
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