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Novel targets in rectal cancer by considering lncRNA–miRNA–mRNA network in response to Lactobacillus acidophilus consumption: a randomized clinical trial
We aimed to explore the lncRNA–miR–mRNA network in response to Lactobacillus acidophilus (L. acidophilus) consumption in rectal cancer patients. The candidate miRs were first taken from the GEO and TCGA databases. We constructed the lncRNA–miR–mRNA network using the high-throughput sequencing data....
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9163174/ https://www.ncbi.nlm.nih.gov/pubmed/35654932 http://dx.doi.org/10.1038/s41598-022-13297-9 |
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author | Khodaii, Zohreh Mehrabani Natanzi, Mahboobeh Khalighfard, Solmaz Ghandian Zanjan, Maziar Gharghi, Maryam Khori, Vahid Amiriani, Taghi Rahimkhani, Monireh Alizadeh, Ali Mohammad |
author_facet | Khodaii, Zohreh Mehrabani Natanzi, Mahboobeh Khalighfard, Solmaz Ghandian Zanjan, Maziar Gharghi, Maryam Khori, Vahid Amiriani, Taghi Rahimkhani, Monireh Alizadeh, Ali Mohammad |
author_sort | Khodaii, Zohreh |
collection | PubMed |
description | We aimed to explore the lncRNA–miR–mRNA network in response to Lactobacillus acidophilus (L. acidophilus) consumption in rectal cancer patients. The candidate miRs were first taken from the GEO and TCGA databases. We constructed the lncRNA–miR–mRNA network using the high-throughput sequencing data. At last, we created a heatmap based on the experimental data to show the possible correlation of the selected targets. The expression levels of selected targets were measured in the samples of 107 rectal cancer patients undergoing placebo and probiotic consumption and 10 noncancerous subjects using Real-Time PCR. Our analysis revealed a group of differentially expressed 12 miRs and 11 lncRNAs, and 12 genes in rectal cancer patients. A significant expression increase of the selected tumor suppressor miRs, lncRNAs, and genes and a substantial expression decrease of the selected oncomiRs, onco-lncRNAs, and oncogenes were obtained after the probiotic consumption compared to the placebo group. There is a strong correlation between some network components, including miR-133b and IGF1 gene, miR-548ac and MSH2 gene, and miR-21 and SMAD4 gene. In rectal cancer patients, L. acidophilus consumption was associated with improved expression of the lncRNA–miR–mRNA network, which may provide novel monitoring and therapeutic approaches. |
format | Online Article Text |
id | pubmed-9163174 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-91631742022-06-05 Novel targets in rectal cancer by considering lncRNA–miRNA–mRNA network in response to Lactobacillus acidophilus consumption: a randomized clinical trial Khodaii, Zohreh Mehrabani Natanzi, Mahboobeh Khalighfard, Solmaz Ghandian Zanjan, Maziar Gharghi, Maryam Khori, Vahid Amiriani, Taghi Rahimkhani, Monireh Alizadeh, Ali Mohammad Sci Rep Article We aimed to explore the lncRNA–miR–mRNA network in response to Lactobacillus acidophilus (L. acidophilus) consumption in rectal cancer patients. The candidate miRs were first taken from the GEO and TCGA databases. We constructed the lncRNA–miR–mRNA network using the high-throughput sequencing data. At last, we created a heatmap based on the experimental data to show the possible correlation of the selected targets. The expression levels of selected targets were measured in the samples of 107 rectal cancer patients undergoing placebo and probiotic consumption and 10 noncancerous subjects using Real-Time PCR. Our analysis revealed a group of differentially expressed 12 miRs and 11 lncRNAs, and 12 genes in rectal cancer patients. A significant expression increase of the selected tumor suppressor miRs, lncRNAs, and genes and a substantial expression decrease of the selected oncomiRs, onco-lncRNAs, and oncogenes were obtained after the probiotic consumption compared to the placebo group. There is a strong correlation between some network components, including miR-133b and IGF1 gene, miR-548ac and MSH2 gene, and miR-21 and SMAD4 gene. In rectal cancer patients, L. acidophilus consumption was associated with improved expression of the lncRNA–miR–mRNA network, which may provide novel monitoring and therapeutic approaches. Nature Publishing Group UK 2022-06-02 /pmc/articles/PMC9163174/ /pubmed/35654932 http://dx.doi.org/10.1038/s41598-022-13297-9 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Khodaii, Zohreh Mehrabani Natanzi, Mahboobeh Khalighfard, Solmaz Ghandian Zanjan, Maziar Gharghi, Maryam Khori, Vahid Amiriani, Taghi Rahimkhani, Monireh Alizadeh, Ali Mohammad Novel targets in rectal cancer by considering lncRNA–miRNA–mRNA network in response to Lactobacillus acidophilus consumption: a randomized clinical trial |
title | Novel targets in rectal cancer by considering lncRNA–miRNA–mRNA network in response to Lactobacillus acidophilus consumption: a randomized clinical trial |
title_full | Novel targets in rectal cancer by considering lncRNA–miRNA–mRNA network in response to Lactobacillus acidophilus consumption: a randomized clinical trial |
title_fullStr | Novel targets in rectal cancer by considering lncRNA–miRNA–mRNA network in response to Lactobacillus acidophilus consumption: a randomized clinical trial |
title_full_unstemmed | Novel targets in rectal cancer by considering lncRNA–miRNA–mRNA network in response to Lactobacillus acidophilus consumption: a randomized clinical trial |
title_short | Novel targets in rectal cancer by considering lncRNA–miRNA–mRNA network in response to Lactobacillus acidophilus consumption: a randomized clinical trial |
title_sort | novel targets in rectal cancer by considering lncrna–mirna–mrna network in response to lactobacillus acidophilus consumption: a randomized clinical trial |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9163174/ https://www.ncbi.nlm.nih.gov/pubmed/35654932 http://dx.doi.org/10.1038/s41598-022-13297-9 |
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