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Modification of adipose mesenchymal stem cells-derived small extracellular vesicles with fibrin-targeting peptide CREKA for enhanced bone repair

The process of bone repair is highly regulated by a large number of bioactive factors. Thus, a “cocktail” of bioactive factors supplemented to the defect sites is desirable for bone repair. In this regard, small extracellular vesicles (sEVs) derived from mesenchymal stem cells hold great potential i...

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Detalles Bibliográficos
Autores principales: Wu, Qi, Fu, Xiaoling, Li, Xian, Li, Jing, Han, Weiju, Wang, Yingjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: KeAi Publishing 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9163428/
https://www.ncbi.nlm.nih.gov/pubmed/35702606
http://dx.doi.org/10.1016/j.bioactmat.2022.05.031
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author Wu, Qi
Fu, Xiaoling
Li, Xian
Li, Jing
Han, Weiju
Wang, Yingjun
author_facet Wu, Qi
Fu, Xiaoling
Li, Xian
Li, Jing
Han, Weiju
Wang, Yingjun
author_sort Wu, Qi
collection PubMed
description The process of bone repair is highly regulated by a large number of bioactive factors. Thus, a “cocktail” of bioactive factors supplemented to the defect sites is desirable for bone repair. In this regard, small extracellular vesicles (sEVs) derived from mesenchymal stem cells hold great potential in tissue repair. Nevertheless, the poor homing and retention of sEVs greatly limited their possible clinical application. In the present work, DMPE-PEG-CREKA was inserted into the membrane of sEVs released from adipose-derived mesenchymal stem cells to obtain CREKA functionalized sEVs (CREKA-sEVs), which could target fibrin to accumulate and retain in bone defects. Our results showed that CREKA-sEVs, like sEVs, promoted the osteogenic differentiation of BMSCs, the angiogenic property of HUVECs, and modulated the polarization of macrophages in vitro. Furthermore, due to the improved fibrin-binding and retention capacity of CREKA-sEVs, they enhanced the bone repair substantially in the rat femoral defect model. This study provided a new strategy to improve the therapeutic efficiency of sEVs and showed that CREKA-sEVs had great application value in bone tissue repair.
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spelling pubmed-91634282022-06-13 Modification of adipose mesenchymal stem cells-derived small extracellular vesicles with fibrin-targeting peptide CREKA for enhanced bone repair Wu, Qi Fu, Xiaoling Li, Xian Li, Jing Han, Weiju Wang, Yingjun Bioact Mater Article The process of bone repair is highly regulated by a large number of bioactive factors. Thus, a “cocktail” of bioactive factors supplemented to the defect sites is desirable for bone repair. In this regard, small extracellular vesicles (sEVs) derived from mesenchymal stem cells hold great potential in tissue repair. Nevertheless, the poor homing and retention of sEVs greatly limited their possible clinical application. In the present work, DMPE-PEG-CREKA was inserted into the membrane of sEVs released from adipose-derived mesenchymal stem cells to obtain CREKA functionalized sEVs (CREKA-sEVs), which could target fibrin to accumulate and retain in bone defects. Our results showed that CREKA-sEVs, like sEVs, promoted the osteogenic differentiation of BMSCs, the angiogenic property of HUVECs, and modulated the polarization of macrophages in vitro. Furthermore, due to the improved fibrin-binding and retention capacity of CREKA-sEVs, they enhanced the bone repair substantially in the rat femoral defect model. This study provided a new strategy to improve the therapeutic efficiency of sEVs and showed that CREKA-sEVs had great application value in bone tissue repair. KeAi Publishing 2022-05-31 /pmc/articles/PMC9163428/ /pubmed/35702606 http://dx.doi.org/10.1016/j.bioactmat.2022.05.031 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Wu, Qi
Fu, Xiaoling
Li, Xian
Li, Jing
Han, Weiju
Wang, Yingjun
Modification of adipose mesenchymal stem cells-derived small extracellular vesicles with fibrin-targeting peptide CREKA for enhanced bone repair
title Modification of adipose mesenchymal stem cells-derived small extracellular vesicles with fibrin-targeting peptide CREKA for enhanced bone repair
title_full Modification of adipose mesenchymal stem cells-derived small extracellular vesicles with fibrin-targeting peptide CREKA for enhanced bone repair
title_fullStr Modification of adipose mesenchymal stem cells-derived small extracellular vesicles with fibrin-targeting peptide CREKA for enhanced bone repair
title_full_unstemmed Modification of adipose mesenchymal stem cells-derived small extracellular vesicles with fibrin-targeting peptide CREKA for enhanced bone repair
title_short Modification of adipose mesenchymal stem cells-derived small extracellular vesicles with fibrin-targeting peptide CREKA for enhanced bone repair
title_sort modification of adipose mesenchymal stem cells-derived small extracellular vesicles with fibrin-targeting peptide creka for enhanced bone repair
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9163428/
https://www.ncbi.nlm.nih.gov/pubmed/35702606
http://dx.doi.org/10.1016/j.bioactmat.2022.05.031
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