Cargando…

Digital Cell Atlas of Mouse Uterus: From Regenerative Stage to Maturational Stage

Endometrium undergoes repeated repair and regeneration during the menstrual cycle. Previous attempts using gene expression data to define the menstrual cycle failed to come to an agreement. Here we used single-cell RNA sequencing data of C57BL/6J mice uteri to construct a novel integrated cell atlas...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Leyi, Long, Wenying, Xu, Wanwan, Chen, Xiuying, Zhao, Xiaofeng, Wu, Bingbing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9163836/
https://www.ncbi.nlm.nih.gov/pubmed/35669188
http://dx.doi.org/10.3389/fgene.2022.847646
_version_ 1784720000260505600
author Zhang, Leyi
Long, Wenying
Xu, Wanwan
Chen, Xiuying
Zhao, Xiaofeng
Wu, Bingbing
author_facet Zhang, Leyi
Long, Wenying
Xu, Wanwan
Chen, Xiuying
Zhao, Xiaofeng
Wu, Bingbing
author_sort Zhang, Leyi
collection PubMed
description Endometrium undergoes repeated repair and regeneration during the menstrual cycle. Previous attempts using gene expression data to define the menstrual cycle failed to come to an agreement. Here we used single-cell RNA sequencing data of C57BL/6J mice uteri to construct a novel integrated cell atlas of mice uteri from the regenerative endometrium to the maturational endometrium at the single-cell level, providing a more accurate cytological-based elucidation for the changes that occurred in the endometrium during the estrus cycle. Based on the expression levels of proliferating cell nuclear antigen, differentially expressed genes, and gene ontology terms, we delineated in detail the transitions of epithelial cells, stromal cells, and immune cells that happened during the estrus cycle. The transcription factors that shaped the differentiation of the mononuclear phagocyte system had been proposed, being Mafb, Irf7, and Nr4a1. The amounts and functions of immune cells varied sharply in two stages, especially NK cells and macrophages. We also found putative uterus tissue-resident macrophages and identified potential endometrial mesenchymal stem cells (high expression of Cd34, Pdgfrb, Aldh1a2) in vivo. The cell atlas of mice uteri presented here would improve our understanding of the transitions that occurred in the endometrium from the regenerative endometrium to the maturational endometrium. With the assistance of a normal cell atlas as a reference, we may identify morphologically unaffected abnormalities in future clinical practice. Cautions would be needed when adopting our conclusions, for the limited number of mice that participated in this study may affect the strength of our conclusions.
format Online
Article
Text
id pubmed-9163836
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-91638362022-06-05 Digital Cell Atlas of Mouse Uterus: From Regenerative Stage to Maturational Stage Zhang, Leyi Long, Wenying Xu, Wanwan Chen, Xiuying Zhao, Xiaofeng Wu, Bingbing Front Genet Genetics Endometrium undergoes repeated repair and regeneration during the menstrual cycle. Previous attempts using gene expression data to define the menstrual cycle failed to come to an agreement. Here we used single-cell RNA sequencing data of C57BL/6J mice uteri to construct a novel integrated cell atlas of mice uteri from the regenerative endometrium to the maturational endometrium at the single-cell level, providing a more accurate cytological-based elucidation for the changes that occurred in the endometrium during the estrus cycle. Based on the expression levels of proliferating cell nuclear antigen, differentially expressed genes, and gene ontology terms, we delineated in detail the transitions of epithelial cells, stromal cells, and immune cells that happened during the estrus cycle. The transcription factors that shaped the differentiation of the mononuclear phagocyte system had been proposed, being Mafb, Irf7, and Nr4a1. The amounts and functions of immune cells varied sharply in two stages, especially NK cells and macrophages. We also found putative uterus tissue-resident macrophages and identified potential endometrial mesenchymal stem cells (high expression of Cd34, Pdgfrb, Aldh1a2) in vivo. The cell atlas of mice uteri presented here would improve our understanding of the transitions that occurred in the endometrium from the regenerative endometrium to the maturational endometrium. With the assistance of a normal cell atlas as a reference, we may identify morphologically unaffected abnormalities in future clinical practice. Cautions would be needed when adopting our conclusions, for the limited number of mice that participated in this study may affect the strength of our conclusions. Frontiers Media S.A. 2022-05-20 /pmc/articles/PMC9163836/ /pubmed/35669188 http://dx.doi.org/10.3389/fgene.2022.847646 Text en Copyright © 2022 Zhang, Long, Xu, Chen, Zhao and Wu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Zhang, Leyi
Long, Wenying
Xu, Wanwan
Chen, Xiuying
Zhao, Xiaofeng
Wu, Bingbing
Digital Cell Atlas of Mouse Uterus: From Regenerative Stage to Maturational Stage
title Digital Cell Atlas of Mouse Uterus: From Regenerative Stage to Maturational Stage
title_full Digital Cell Atlas of Mouse Uterus: From Regenerative Stage to Maturational Stage
title_fullStr Digital Cell Atlas of Mouse Uterus: From Regenerative Stage to Maturational Stage
title_full_unstemmed Digital Cell Atlas of Mouse Uterus: From Regenerative Stage to Maturational Stage
title_short Digital Cell Atlas of Mouse Uterus: From Regenerative Stage to Maturational Stage
title_sort digital cell atlas of mouse uterus: from regenerative stage to maturational stage
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9163836/
https://www.ncbi.nlm.nih.gov/pubmed/35669188
http://dx.doi.org/10.3389/fgene.2022.847646
work_keys_str_mv AT zhangleyi digitalcellatlasofmouseuterusfromregenerativestagetomaturationalstage
AT longwenying digitalcellatlasofmouseuterusfromregenerativestagetomaturationalstage
AT xuwanwan digitalcellatlasofmouseuterusfromregenerativestagetomaturationalstage
AT chenxiuying digitalcellatlasofmouseuterusfromregenerativestagetomaturationalstage
AT zhaoxiaofeng digitalcellatlasofmouseuterusfromregenerativestagetomaturationalstage
AT wubingbing digitalcellatlasofmouseuterusfromregenerativestagetomaturationalstage