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Human Cardiac Progenitor Cell-Derived Extracellular Vesicles Exhibit Promising Potential for Supporting Cardiac Repair in Vitro

Although human Cardiac Progenitor Cells (hCPCs) are not retained by host myocardium they still improve cardiac function when injected into ischemic heart. Emerging evidence supports the hypothesis that hCPC beneficial effects are induced by paracrine action on resident cells. Extracellular vesicles...

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Autores principales: Romano, Veronica, Belviso, Immacolata, Sacco, Anna Maria, Cozzolino, Domenico, Nurzynska, Daria, Amarelli, Cristiano, Maiello, Ciro, Sirico, Felice, Di Meglio, Franca, Castaldo, Clotilde
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9163838/
https://www.ncbi.nlm.nih.gov/pubmed/35669580
http://dx.doi.org/10.3389/fphys.2022.879046
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author Romano, Veronica
Belviso, Immacolata
Sacco, Anna Maria
Cozzolino, Domenico
Nurzynska, Daria
Amarelli, Cristiano
Maiello, Ciro
Sirico, Felice
Di Meglio, Franca
Castaldo, Clotilde
author_facet Romano, Veronica
Belviso, Immacolata
Sacco, Anna Maria
Cozzolino, Domenico
Nurzynska, Daria
Amarelli, Cristiano
Maiello, Ciro
Sirico, Felice
Di Meglio, Franca
Castaldo, Clotilde
author_sort Romano, Veronica
collection PubMed
description Although human Cardiac Progenitor Cells (hCPCs) are not retained by host myocardium they still improve cardiac function when injected into ischemic heart. Emerging evidence supports the hypothesis that hCPC beneficial effects are induced by paracrine action on resident cells. Extracellular vesicles (EVs) are an intriguing mechanism of cell communication based on the transport and transfer of peptides, lipids, and nucleic acids that have the potential to modulate signaling pathways, cell growth, migration, and proliferation of recipient cells. We hypothesize that EVs are involved in the paracrine effects elicited by hCPCs and held accountable for the response of the infarcted myocardium to hCPC-based cell therapy. To test this theory, we collected EVs released by hCPCs isolated from healthy myocardium and evaluated the effects they elicited when administered to resident hCPC and cardiac fibroblasts (CFs) isolated from patients with post-ischemic end-stage heart failure. Evidence emerging from our study indicated that hCPC-derived EVs impacted upon proliferation and survival of hCPCs residing in the ischemic heart and regulated the synthesis and deposition of extracellular-matrix by CFs. These findings suggest that beneficial effects exerted by hCPC injection are, at least to some extent, ascribable to the delivery of signals conveyed by EVs.
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spelling pubmed-91638382022-06-05 Human Cardiac Progenitor Cell-Derived Extracellular Vesicles Exhibit Promising Potential for Supporting Cardiac Repair in Vitro Romano, Veronica Belviso, Immacolata Sacco, Anna Maria Cozzolino, Domenico Nurzynska, Daria Amarelli, Cristiano Maiello, Ciro Sirico, Felice Di Meglio, Franca Castaldo, Clotilde Front Physiol Physiology Although human Cardiac Progenitor Cells (hCPCs) are not retained by host myocardium they still improve cardiac function when injected into ischemic heart. Emerging evidence supports the hypothesis that hCPC beneficial effects are induced by paracrine action on resident cells. Extracellular vesicles (EVs) are an intriguing mechanism of cell communication based on the transport and transfer of peptides, lipids, and nucleic acids that have the potential to modulate signaling pathways, cell growth, migration, and proliferation of recipient cells. We hypothesize that EVs are involved in the paracrine effects elicited by hCPCs and held accountable for the response of the infarcted myocardium to hCPC-based cell therapy. To test this theory, we collected EVs released by hCPCs isolated from healthy myocardium and evaluated the effects they elicited when administered to resident hCPC and cardiac fibroblasts (CFs) isolated from patients with post-ischemic end-stage heart failure. Evidence emerging from our study indicated that hCPC-derived EVs impacted upon proliferation and survival of hCPCs residing in the ischemic heart and regulated the synthesis and deposition of extracellular-matrix by CFs. These findings suggest that beneficial effects exerted by hCPC injection are, at least to some extent, ascribable to the delivery of signals conveyed by EVs. Frontiers Media S.A. 2022-05-20 /pmc/articles/PMC9163838/ /pubmed/35669580 http://dx.doi.org/10.3389/fphys.2022.879046 Text en Copyright © 2022 Romano, Belviso, Sacco, Cozzolino, Nurzynska, Amarelli, Maiello, Sirico, Di Meglio and Castaldo. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Romano, Veronica
Belviso, Immacolata
Sacco, Anna Maria
Cozzolino, Domenico
Nurzynska, Daria
Amarelli, Cristiano
Maiello, Ciro
Sirico, Felice
Di Meglio, Franca
Castaldo, Clotilde
Human Cardiac Progenitor Cell-Derived Extracellular Vesicles Exhibit Promising Potential for Supporting Cardiac Repair in Vitro
title Human Cardiac Progenitor Cell-Derived Extracellular Vesicles Exhibit Promising Potential for Supporting Cardiac Repair in Vitro
title_full Human Cardiac Progenitor Cell-Derived Extracellular Vesicles Exhibit Promising Potential for Supporting Cardiac Repair in Vitro
title_fullStr Human Cardiac Progenitor Cell-Derived Extracellular Vesicles Exhibit Promising Potential for Supporting Cardiac Repair in Vitro
title_full_unstemmed Human Cardiac Progenitor Cell-Derived Extracellular Vesicles Exhibit Promising Potential for Supporting Cardiac Repair in Vitro
title_short Human Cardiac Progenitor Cell-Derived Extracellular Vesicles Exhibit Promising Potential for Supporting Cardiac Repair in Vitro
title_sort human cardiac progenitor cell-derived extracellular vesicles exhibit promising potential for supporting cardiac repair in vitro
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9163838/
https://www.ncbi.nlm.nih.gov/pubmed/35669580
http://dx.doi.org/10.3389/fphys.2022.879046
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