Analysis and validation of silica-immobilised BST polymerase in loop-mediated isothermal amplification (LAMP) for malaria diagnosis

Bacillus stearothermophilus large fragment (BST(LF)) DNA polymerase is reported, isolated on silica via a fused R5 silica-affinity peptide and used in nucleic acid diagnostics. mCherry (mCh), included in the fusion construct, was shown as an efficient fluorescent label to follow the workflow from ge...

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Detalles Bibliográficos
Autores principales: Seevaratnam, Dushanth, Ansah, Felix, Aniweh, Yaw, Awandare, Gordon A., Hall, Elizabeth A. H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2022
Materias:
Paper in Forefront
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9163865/
https://www.ncbi.nlm.nih.gov/pubmed/35657389
http://dx.doi.org/10.1007/s00216-022-04131-2

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