Cargando…
Pharmacokinetic Assessment of Staphylococcal Phage K Following Parenteral and Intra-articular Administration in Rabbits
The therapeutic value of phage as an alternative to antibiotics for the treatment of bacterial infections is being considered in the wake of mounting antibiotic resistance. In this study, the pharmacokinetic properties of Staphylococcus aureus phage K following intravenous and intra-articular admini...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9163985/ https://www.ncbi.nlm.nih.gov/pubmed/35668926 http://dx.doi.org/10.3389/fphar.2022.840165 |
_version_ | 1784720036673355776 |
---|---|
author | Totten, Katherine M.C. Cunningham, Scott A. Gades, Naomi M. Etzioni, Athema Patel, Robin |
author_facet | Totten, Katherine M.C. Cunningham, Scott A. Gades, Naomi M. Etzioni, Athema Patel, Robin |
author_sort | Totten, Katherine M.C. |
collection | PubMed |
description | The therapeutic value of phage as an alternative to antibiotics for the treatment of bacterial infections is being considered in the wake of mounting antibiotic resistance. In this study, the pharmacokinetic properties of Staphylococcus aureus phage K following intravenous and intra-articular administration were investigated in a rabbit model. Using a traditional plaque assay and a novel quantitative PCR assay to measure phage levels in specimens over time, it was found that intra-articularly administered phage enters the systemic circulation; that phage may be detected in synovial fluid up to 24 h following the intra-articular, but not intravenous, administration; and that qPCR-based enumeration is generally more sensitive than plaque enumeration, with fair to moderate correlation between the two methods. Findings presented should inform the design of phage therapy experiments and therapeutic drug monitoring in preclinical and human phage studies. |
format | Online Article Text |
id | pubmed-9163985 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-91639852022-06-05 Pharmacokinetic Assessment of Staphylococcal Phage K Following Parenteral and Intra-articular Administration in Rabbits Totten, Katherine M.C. Cunningham, Scott A. Gades, Naomi M. Etzioni, Athema Patel, Robin Front Pharmacol Pharmacology The therapeutic value of phage as an alternative to antibiotics for the treatment of bacterial infections is being considered in the wake of mounting antibiotic resistance. In this study, the pharmacokinetic properties of Staphylococcus aureus phage K following intravenous and intra-articular administration were investigated in a rabbit model. Using a traditional plaque assay and a novel quantitative PCR assay to measure phage levels in specimens over time, it was found that intra-articularly administered phage enters the systemic circulation; that phage may be detected in synovial fluid up to 24 h following the intra-articular, but not intravenous, administration; and that qPCR-based enumeration is generally more sensitive than plaque enumeration, with fair to moderate correlation between the two methods. Findings presented should inform the design of phage therapy experiments and therapeutic drug monitoring in preclinical and human phage studies. Frontiers Media S.A. 2022-05-20 /pmc/articles/PMC9163985/ /pubmed/35668926 http://dx.doi.org/10.3389/fphar.2022.840165 Text en Copyright © 2022 Totten, Cunningham, Gades, Etzioni and Patel. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Pharmacology Totten, Katherine M.C. Cunningham, Scott A. Gades, Naomi M. Etzioni, Athema Patel, Robin Pharmacokinetic Assessment of Staphylococcal Phage K Following Parenteral and Intra-articular Administration in Rabbits |
title | Pharmacokinetic Assessment of Staphylococcal Phage K Following Parenteral and Intra-articular Administration in Rabbits |
title_full | Pharmacokinetic Assessment of Staphylococcal Phage K Following Parenteral and Intra-articular Administration in Rabbits |
title_fullStr | Pharmacokinetic Assessment of Staphylococcal Phage K Following Parenteral and Intra-articular Administration in Rabbits |
title_full_unstemmed | Pharmacokinetic Assessment of Staphylococcal Phage K Following Parenteral and Intra-articular Administration in Rabbits |
title_short | Pharmacokinetic Assessment of Staphylococcal Phage K Following Parenteral and Intra-articular Administration in Rabbits |
title_sort | pharmacokinetic assessment of staphylococcal phage k following parenteral and intra-articular administration in rabbits |
topic | Pharmacology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9163985/ https://www.ncbi.nlm.nih.gov/pubmed/35668926 http://dx.doi.org/10.3389/fphar.2022.840165 |
work_keys_str_mv | AT tottenkatherinemc pharmacokineticassessmentofstaphylococcalphagekfollowingparenteralandintraarticularadministrationinrabbits AT cunninghamscotta pharmacokineticassessmentofstaphylococcalphagekfollowingparenteralandintraarticularadministrationinrabbits AT gadesnaomim pharmacokineticassessmentofstaphylococcalphagekfollowingparenteralandintraarticularadministrationinrabbits AT etzioniathema pharmacokineticassessmentofstaphylococcalphagekfollowingparenteralandintraarticularadministrationinrabbits AT patelrobin pharmacokineticassessmentofstaphylococcalphagekfollowingparenteralandintraarticularadministrationinrabbits |