Cargando…
Competitive Hybridization of a Microarray Identifies CMKLR1 as an Up-Regulated Gene in Human Bone Marrow-Derived Mesenchymal Stem Cells Compared to Human Embryonic Fibroblasts
Mesenchymal stem cells (MSCs) have been widely applied to the regeneration of damaged tissue and the modulation of immune response. The purity of MSC preparation and the delivery of MSCs to a target region are critical factors for success in therapeutic application. In order to define the molecular...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9164045/ https://www.ncbi.nlm.nih.gov/pubmed/35723360 http://dx.doi.org/10.3390/cimb44040102 |
_version_ | 1784720051098615808 |
---|---|
author | Cho, Hee-Yeon Lee, Sooho Park, Ji-Hong Kwak, Yoon Hae Kweon, HaeYong Kang, Dongchul |
author_facet | Cho, Hee-Yeon Lee, Sooho Park, Ji-Hong Kwak, Yoon Hae Kweon, HaeYong Kang, Dongchul |
author_sort | Cho, Hee-Yeon |
collection | PubMed |
description | Mesenchymal stem cells (MSCs) have been widely applied to the regeneration of damaged tissue and the modulation of immune response. The purity of MSC preparation and the delivery of MSCs to a target region are critical factors for success in therapeutic application. In order to define the molecular identity of an MSC, the gene expression pattern of a human bone marrow-derived mesenchymal stem cell (hBMSC) was compared with that of a human embryonic fibroblast (hEF) by competitive hybridization of a microarray. A total of 270 and 173 genes were two-fold up- and down-regulated with FDR < 0.05 in the hBMSC compared to the hEF, respectively. The overexpressed genes in the hBMSC over the hEF, including transcription factors, were enriched for biological processes such as axial pattern formation, face morphogenesis and skeletal system development, which could be expected from the differentiation potential of MSCs. CD70 and CD339 were identified as additional CD markers that were up-regulated in the hBMSC over the hEF. The differential expression of CD70 and CD339 might be exploited to distinguish hEF and hBMSC. CMKLR1, a chemokine receptor, was up-regulated in the hBMSC compared to the hEF. RARRES2, a CMKLR1 ligand, stimulated specific migration of the hBMSC, but not of the hEF. RARRES2 manifested as ~two-fold less effective than SDF-1α in the directional migration of the hBMSC. The expression of CMKLR1 was decreased upon the osteoblastic differentiation of the hBMSC. However, the RARRES2-loaded 10% HA-silk scaffold did not recruit endogenous cells to the scaffold in vivo. The RARRES2–CMKLR1 axis could be employed in recruiting systemically delivered or endogenous MSCs to a specific target lesion. |
format | Online Article Text |
id | pubmed-9164045 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91640452022-06-04 Competitive Hybridization of a Microarray Identifies CMKLR1 as an Up-Regulated Gene in Human Bone Marrow-Derived Mesenchymal Stem Cells Compared to Human Embryonic Fibroblasts Cho, Hee-Yeon Lee, Sooho Park, Ji-Hong Kwak, Yoon Hae Kweon, HaeYong Kang, Dongchul Curr Issues Mol Biol Article Mesenchymal stem cells (MSCs) have been widely applied to the regeneration of damaged tissue and the modulation of immune response. The purity of MSC preparation and the delivery of MSCs to a target region are critical factors for success in therapeutic application. In order to define the molecular identity of an MSC, the gene expression pattern of a human bone marrow-derived mesenchymal stem cell (hBMSC) was compared with that of a human embryonic fibroblast (hEF) by competitive hybridization of a microarray. A total of 270 and 173 genes were two-fold up- and down-regulated with FDR < 0.05 in the hBMSC compared to the hEF, respectively. The overexpressed genes in the hBMSC over the hEF, including transcription factors, were enriched for biological processes such as axial pattern formation, face morphogenesis and skeletal system development, which could be expected from the differentiation potential of MSCs. CD70 and CD339 were identified as additional CD markers that were up-regulated in the hBMSC over the hEF. The differential expression of CD70 and CD339 might be exploited to distinguish hEF and hBMSC. CMKLR1, a chemokine receptor, was up-regulated in the hBMSC compared to the hEF. RARRES2, a CMKLR1 ligand, stimulated specific migration of the hBMSC, but not of the hEF. RARRES2 manifested as ~two-fold less effective than SDF-1α in the directional migration of the hBMSC. The expression of CMKLR1 was decreased upon the osteoblastic differentiation of the hBMSC. However, the RARRES2-loaded 10% HA-silk scaffold did not recruit endogenous cells to the scaffold in vivo. The RARRES2–CMKLR1 axis could be employed in recruiting systemically delivered or endogenous MSCs to a specific target lesion. MDPI 2022-03-28 /pmc/articles/PMC9164045/ /pubmed/35723360 http://dx.doi.org/10.3390/cimb44040102 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Cho, Hee-Yeon Lee, Sooho Park, Ji-Hong Kwak, Yoon Hae Kweon, HaeYong Kang, Dongchul Competitive Hybridization of a Microarray Identifies CMKLR1 as an Up-Regulated Gene in Human Bone Marrow-Derived Mesenchymal Stem Cells Compared to Human Embryonic Fibroblasts |
title | Competitive Hybridization of a Microarray Identifies CMKLR1 as an Up-Regulated Gene in Human Bone Marrow-Derived Mesenchymal Stem Cells Compared to Human Embryonic Fibroblasts |
title_full | Competitive Hybridization of a Microarray Identifies CMKLR1 as an Up-Regulated Gene in Human Bone Marrow-Derived Mesenchymal Stem Cells Compared to Human Embryonic Fibroblasts |
title_fullStr | Competitive Hybridization of a Microarray Identifies CMKLR1 as an Up-Regulated Gene in Human Bone Marrow-Derived Mesenchymal Stem Cells Compared to Human Embryonic Fibroblasts |
title_full_unstemmed | Competitive Hybridization of a Microarray Identifies CMKLR1 as an Up-Regulated Gene in Human Bone Marrow-Derived Mesenchymal Stem Cells Compared to Human Embryonic Fibroblasts |
title_short | Competitive Hybridization of a Microarray Identifies CMKLR1 as an Up-Regulated Gene in Human Bone Marrow-Derived Mesenchymal Stem Cells Compared to Human Embryonic Fibroblasts |
title_sort | competitive hybridization of a microarray identifies cmklr1 as an up-regulated gene in human bone marrow-derived mesenchymal stem cells compared to human embryonic fibroblasts |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9164045/ https://www.ncbi.nlm.nih.gov/pubmed/35723360 http://dx.doi.org/10.3390/cimb44040102 |
work_keys_str_mv | AT choheeyeon competitivehybridizationofamicroarrayidentifiescmklr1asanupregulatedgeneinhumanbonemarrowderivedmesenchymalstemcellscomparedtohumanembryonicfibroblasts AT leesooho competitivehybridizationofamicroarrayidentifiescmklr1asanupregulatedgeneinhumanbonemarrowderivedmesenchymalstemcellscomparedtohumanembryonicfibroblasts AT parkjihong competitivehybridizationofamicroarrayidentifiescmklr1asanupregulatedgeneinhumanbonemarrowderivedmesenchymalstemcellscomparedtohumanembryonicfibroblasts AT kwakyoonhae competitivehybridizationofamicroarrayidentifiescmklr1asanupregulatedgeneinhumanbonemarrowderivedmesenchymalstemcellscomparedtohumanembryonicfibroblasts AT kweonhaeyong competitivehybridizationofamicroarrayidentifiescmklr1asanupregulatedgeneinhumanbonemarrowderivedmesenchymalstemcellscomparedtohumanembryonicfibroblasts AT kangdongchul competitivehybridizationofamicroarrayidentifiescmklr1asanupregulatedgeneinhumanbonemarrowderivedmesenchymalstemcellscomparedtohumanembryonicfibroblasts |