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In Ovo Injection of CHIR-99021 Promotes Feather Follicle Development via Modulating the Wnt Signaling Pathway and Transcriptome in Goose Embryos (Anser cygnoides)
Feather performs important physiological functions in birds, and it is also one of the economic productions in goose farming. Understanding and modulating feather follicle development during embryogenesis are essential for bird biology and the poultry industry. CHIR-99021 is a potent Wnt/β-catenin s...
| Autores principales: | , , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Frontiers Media S.A.
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9164139/ https://www.ncbi.nlm.nih.gov/pubmed/35669574 http://dx.doi.org/10.3389/fphys.2022.858274 |
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| author | Feng, Ziqiang Gong, Haizhou Fu, Jinhong Xu, Xiaohui Song, Yupu Yan, Xiaomin Mabrouk, Ichraf Zhou, Yuxuan Wang, Yudong Fu, Xianou Sui, Yujian Liu, Tuoya Li, Chuanghang Liu, Zebei Tian, Xu Sun, Le Guo, Keying Sun, Yongfeng Hu, Jingtao |
| author_facet | Feng, Ziqiang Gong, Haizhou Fu, Jinhong Xu, Xiaohui Song, Yupu Yan, Xiaomin Mabrouk, Ichraf Zhou, Yuxuan Wang, Yudong Fu, Xianou Sui, Yujian Liu, Tuoya Li, Chuanghang Liu, Zebei Tian, Xu Sun, Le Guo, Keying Sun, Yongfeng Hu, Jingtao |
| author_sort | Feng, Ziqiang |
| collection | PubMed |
| description | Feather performs important physiological functions in birds, and it is also one of the economic productions in goose farming. Understanding and modulating feather follicle development during embryogenesis are essential for bird biology and the poultry industry. CHIR-99021 is a potent Wnt/β-catenin signaling pathway activator associated with feather follicle development. In this study, goose embryos (Anser cygnoides) received an in ovo injection of CHIR-9902, which was conducted at the beginning of feather follicle development (E9). The results showed that feather growth and feather follicle development were promoted. The Wnt signaling pathway was activated by the inhibition of GSK-3β. Transcriptomic analyses showed that the transcription changes were related to translation, metabolism, energy transport, and stress in dorsal tissue of embryos that received CHIR-99021, which might be to adapt and coordinate the promoting effects of CHIR-99021 on feather follicle development. This study suggests that in ovo injection of CHIR-99021 is a potential strategy to improve feather follicle development and feather-related traits for goose farming and provides profiling of the Wnt signaling pathway and transcriptome in dorsal tissue of goose embryos for further understanding of feather follicle development. |
| format | Online Article Text |
| id | pubmed-9164139 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2022 |
| publisher | Frontiers Media S.A. |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-91641392022-06-05 In Ovo Injection of CHIR-99021 Promotes Feather Follicle Development via Modulating the Wnt Signaling Pathway and Transcriptome in Goose Embryos (Anser cygnoides) Feng, Ziqiang Gong, Haizhou Fu, Jinhong Xu, Xiaohui Song, Yupu Yan, Xiaomin Mabrouk, Ichraf Zhou, Yuxuan Wang, Yudong Fu, Xianou Sui, Yujian Liu, Tuoya Li, Chuanghang Liu, Zebei Tian, Xu Sun, Le Guo, Keying Sun, Yongfeng Hu, Jingtao Front Physiol Physiology Feather performs important physiological functions in birds, and it is also one of the economic productions in goose farming. Understanding and modulating feather follicle development during embryogenesis are essential for bird biology and the poultry industry. CHIR-99021 is a potent Wnt/β-catenin signaling pathway activator associated with feather follicle development. In this study, goose embryos (Anser cygnoides) received an in ovo injection of CHIR-9902, which was conducted at the beginning of feather follicle development (E9). The results showed that feather growth and feather follicle development were promoted. The Wnt signaling pathway was activated by the inhibition of GSK-3β. Transcriptomic analyses showed that the transcription changes were related to translation, metabolism, energy transport, and stress in dorsal tissue of embryos that received CHIR-99021, which might be to adapt and coordinate the promoting effects of CHIR-99021 on feather follicle development. This study suggests that in ovo injection of CHIR-99021 is a potential strategy to improve feather follicle development and feather-related traits for goose farming and provides profiling of the Wnt signaling pathway and transcriptome in dorsal tissue of goose embryos for further understanding of feather follicle development. Frontiers Media S.A. 2022-05-20 /pmc/articles/PMC9164139/ /pubmed/35669574 http://dx.doi.org/10.3389/fphys.2022.858274 Text en Copyright © 2022 Feng, Gong, Fu, Xu, Song, Yan, Mabrouk, Zhou, Wang, Fu, Sui, Liu, Li, Liu, Tian, Sun, Guo, Sun and Hu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
| spellingShingle | Physiology Feng, Ziqiang Gong, Haizhou Fu, Jinhong Xu, Xiaohui Song, Yupu Yan, Xiaomin Mabrouk, Ichraf Zhou, Yuxuan Wang, Yudong Fu, Xianou Sui, Yujian Liu, Tuoya Li, Chuanghang Liu, Zebei Tian, Xu Sun, Le Guo, Keying Sun, Yongfeng Hu, Jingtao In Ovo Injection of CHIR-99021 Promotes Feather Follicle Development via Modulating the Wnt Signaling Pathway and Transcriptome in Goose Embryos (Anser cygnoides) |
| title |
In Ovo Injection of CHIR-99021 Promotes Feather Follicle Development via Modulating the Wnt Signaling Pathway and Transcriptome in Goose Embryos (Anser cygnoides) |
| title_full |
In Ovo Injection of CHIR-99021 Promotes Feather Follicle Development via Modulating the Wnt Signaling Pathway and Transcriptome in Goose Embryos (Anser cygnoides) |
| title_fullStr |
In Ovo Injection of CHIR-99021 Promotes Feather Follicle Development via Modulating the Wnt Signaling Pathway and Transcriptome in Goose Embryos (Anser cygnoides) |
| title_full_unstemmed |
In Ovo Injection of CHIR-99021 Promotes Feather Follicle Development via Modulating the Wnt Signaling Pathway and Transcriptome in Goose Embryos (Anser cygnoides) |
| title_short |
In Ovo Injection of CHIR-99021 Promotes Feather Follicle Development via Modulating the Wnt Signaling Pathway and Transcriptome in Goose Embryos (Anser cygnoides) |
| title_sort | in ovo injection of chir-99021 promotes feather follicle development via modulating the wnt signaling pathway and transcriptome in goose embryos (anser cygnoides) |
| topic | Physiology |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9164139/ https://www.ncbi.nlm.nih.gov/pubmed/35669574 http://dx.doi.org/10.3389/fphys.2022.858274 |
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