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Single-cell sequencing analysis reveals gastric cancer microenvironment cells respond vastly different to oxidative stress
Gastric cancer is a common type of gastrointestinal malignant tumor in China. The mechanism of the development and progression of gastric cancer remains the continuing research focus. The tumor microenvironment plays an important role in the development and progression of tumors. The present study u...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9164398/ https://www.ncbi.nlm.nih.gov/pubmed/35659682 http://dx.doi.org/10.1186/s12967-022-03411-w |
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author | Yu, Weihua Chen, Guojun Yan, Jiafei Wang, Xianfa Zhu, Yiping Zhu, Linghua |
author_facet | Yu, Weihua Chen, Guojun Yan, Jiafei Wang, Xianfa Zhu, Yiping Zhu, Linghua |
author_sort | Yu, Weihua |
collection | PubMed |
description | Gastric cancer is a common type of gastrointestinal malignant tumor in China. The mechanism of the development and progression of gastric cancer remains the continuing research focus. The tumor microenvironment plays an important role in the development and progression of tumors. The present study used single-cell sequencing data to characterize the microenvironment of gastric cancer, investigate the effects of oxidative stress on gastric cancer microenvironmental cells through the comparison between cancer tissue and normal tissue, and identify the key genes associated with gastric cancer patients’ survival. The results showed that compared with normal gastric tissue, gastric cancer tissue had a decreased oxidative stress response, weaker oxidative detoxification ability, and increased oxidative stress-induced cell death. In the different types of single cells of gastric cancer microenvironment, the oxidative stress response of T cell was increased, the ability of oxidative detoxification was enhanced, and the oxidative stress-induced cell death was exacerbate. Mucous cell showed the same trend as gastric cancer cells: decreased oxidative stress response, weak oxidative detoxification ability, and weakened oxidative stress-induced cell death. Moreover, TRIM62, MET, and HBA1, which were significantly associated with oxidative stress, may be biomarkers for the prognosis of gastric cancer. High expression of TRIM62 indicated a good prognosis, while MET and HBA1 indicated a poor prognosis, which will be confirmed by further clinical studies. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12967-022-03411-w. |
format | Online Article Text |
id | pubmed-9164398 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-91643982022-06-05 Single-cell sequencing analysis reveals gastric cancer microenvironment cells respond vastly different to oxidative stress Yu, Weihua Chen, Guojun Yan, Jiafei Wang, Xianfa Zhu, Yiping Zhu, Linghua J Transl Med Research Gastric cancer is a common type of gastrointestinal malignant tumor in China. The mechanism of the development and progression of gastric cancer remains the continuing research focus. The tumor microenvironment plays an important role in the development and progression of tumors. The present study used single-cell sequencing data to characterize the microenvironment of gastric cancer, investigate the effects of oxidative stress on gastric cancer microenvironmental cells through the comparison between cancer tissue and normal tissue, and identify the key genes associated with gastric cancer patients’ survival. The results showed that compared with normal gastric tissue, gastric cancer tissue had a decreased oxidative stress response, weaker oxidative detoxification ability, and increased oxidative stress-induced cell death. In the different types of single cells of gastric cancer microenvironment, the oxidative stress response of T cell was increased, the ability of oxidative detoxification was enhanced, and the oxidative stress-induced cell death was exacerbate. Mucous cell showed the same trend as gastric cancer cells: decreased oxidative stress response, weak oxidative detoxification ability, and weakened oxidative stress-induced cell death. Moreover, TRIM62, MET, and HBA1, which were significantly associated with oxidative stress, may be biomarkers for the prognosis of gastric cancer. High expression of TRIM62 indicated a good prognosis, while MET and HBA1 indicated a poor prognosis, which will be confirmed by further clinical studies. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12967-022-03411-w. BioMed Central 2022-06-03 /pmc/articles/PMC9164398/ /pubmed/35659682 http://dx.doi.org/10.1186/s12967-022-03411-w Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Yu, Weihua Chen, Guojun Yan, Jiafei Wang, Xianfa Zhu, Yiping Zhu, Linghua Single-cell sequencing analysis reveals gastric cancer microenvironment cells respond vastly different to oxidative stress |
title | Single-cell sequencing analysis reveals gastric cancer microenvironment cells respond vastly different to oxidative stress |
title_full | Single-cell sequencing analysis reveals gastric cancer microenvironment cells respond vastly different to oxidative stress |
title_fullStr | Single-cell sequencing analysis reveals gastric cancer microenvironment cells respond vastly different to oxidative stress |
title_full_unstemmed | Single-cell sequencing analysis reveals gastric cancer microenvironment cells respond vastly different to oxidative stress |
title_short | Single-cell sequencing analysis reveals gastric cancer microenvironment cells respond vastly different to oxidative stress |
title_sort | single-cell sequencing analysis reveals gastric cancer microenvironment cells respond vastly different to oxidative stress |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9164398/ https://www.ncbi.nlm.nih.gov/pubmed/35659682 http://dx.doi.org/10.1186/s12967-022-03411-w |
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