Transcriptome-wide Cas13 guide RNA design for model organisms and viral RNA pathogens

The recent characterization of RNA-targeting CRISPR nucleases has enabled diverse transcriptome engineering and screening applications that depend crucially on prediction and selection of optimized CRISPR guide RNAs (gRNAs). Previously, we developed a computational model to predict RfxCas13d gRNA ac...

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Detalles Bibliográficos
Autores principales: Guo, Xinyi, Rahman, Jahan A., Wessels, Hans-Hermann, Méndez-Mancilla, Alejandro, Haro, Daniel, Chen, Xinru, Sanjana, Neville E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Resource
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9164475/
https://www.ncbi.nlm.nih.gov/pubmed/35664829
http://dx.doi.org/10.1016/j.xgen.2021.100001

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