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Characterisation of autophagy disruption in the ileum of pigs infected with Lawsonia intracellularis

Lawsonia intracellularis is the aetiological agent of proliferative enteropathy, an enteric disease endemic in swine. Survival in its intracellular niche of the ileum epithelial lining requires the capacity to subvert, repress or exploit the host immune response to create an environment conducive to...

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Autores principales: Salvesen, Hamish A., Sargison, Fiona A., Archibald, Alan L., Ait-Ali, Tahar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9165227/
https://www.ncbi.nlm.nih.gov/pubmed/34669106
http://dx.doi.org/10.1007/s11259-021-09847-7
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author Salvesen, Hamish A.
Sargison, Fiona A.
Archibald, Alan L.
Ait-Ali, Tahar
author_facet Salvesen, Hamish A.
Sargison, Fiona A.
Archibald, Alan L.
Ait-Ali, Tahar
author_sort Salvesen, Hamish A.
collection PubMed
description Lawsonia intracellularis is the aetiological agent of proliferative enteropathy, an enteric disease endemic in swine. Survival in its intracellular niche of the ileum epithelial lining requires the capacity to subvert, repress or exploit the host immune response to create an environment conducive to bacterial propagation. To better understand how L. intracellularis survives in its intracellular niche, we have performed an investigation into the dynamic relationship between infection and the host autophagy response by immunohistochemistry in experimentally infected porcine ileum samples. Beclin1, a protein required early in the autophagy pathway was observed to be distributed with a basal to apical concentration gradient in the crypts of healthy piglets, whilst infected piglets were observed to have no gradient of distribution and an increase in the presence of Beclin1 in crypts with histological characteristics of L. intracellularis residence. Detecting microtubule-associated protein light chain 3 (LC3) is used as a method for monitoring autophagy progression as it associates with mature autophagosomes. For LC3 there was no notable change in signal intensity between crypts with characteristic L. intracellularis infection and healthy crypts of uninfected pigs. Finally, as p62 is degraded with the internal substrate of an autophagosome it was used to measure autophagic flux. There was no observed reduction or redistribution of p62. These preliminary results of the autophagy response in the ileum suggest that L. intracellularis affects autophagy. This disruption to host ileum homeostasis may provide a mechanism that assists in bacterial propagation and contributes to pathogenesis.
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spelling pubmed-91652272022-06-05 Characterisation of autophagy disruption in the ileum of pigs infected with Lawsonia intracellularis Salvesen, Hamish A. Sargison, Fiona A. Archibald, Alan L. Ait-Ali, Tahar Vet Res Commun Short Communication Lawsonia intracellularis is the aetiological agent of proliferative enteropathy, an enteric disease endemic in swine. Survival in its intracellular niche of the ileum epithelial lining requires the capacity to subvert, repress or exploit the host immune response to create an environment conducive to bacterial propagation. To better understand how L. intracellularis survives in its intracellular niche, we have performed an investigation into the dynamic relationship between infection and the host autophagy response by immunohistochemistry in experimentally infected porcine ileum samples. Beclin1, a protein required early in the autophagy pathway was observed to be distributed with a basal to apical concentration gradient in the crypts of healthy piglets, whilst infected piglets were observed to have no gradient of distribution and an increase in the presence of Beclin1 in crypts with histological characteristics of L. intracellularis residence. Detecting microtubule-associated protein light chain 3 (LC3) is used as a method for monitoring autophagy progression as it associates with mature autophagosomes. For LC3 there was no notable change in signal intensity between crypts with characteristic L. intracellularis infection and healthy crypts of uninfected pigs. Finally, as p62 is degraded with the internal substrate of an autophagosome it was used to measure autophagic flux. There was no observed reduction or redistribution of p62. These preliminary results of the autophagy response in the ileum suggest that L. intracellularis affects autophagy. This disruption to host ileum homeostasis may provide a mechanism that assists in bacterial propagation and contributes to pathogenesis. Springer Netherlands 2021-10-20 2022 /pmc/articles/PMC9165227/ /pubmed/34669106 http://dx.doi.org/10.1007/s11259-021-09847-7 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Short Communication
Salvesen, Hamish A.
Sargison, Fiona A.
Archibald, Alan L.
Ait-Ali, Tahar
Characterisation of autophagy disruption in the ileum of pigs infected with Lawsonia intracellularis
title Characterisation of autophagy disruption in the ileum of pigs infected with Lawsonia intracellularis
title_full Characterisation of autophagy disruption in the ileum of pigs infected with Lawsonia intracellularis
title_fullStr Characterisation of autophagy disruption in the ileum of pigs infected with Lawsonia intracellularis
title_full_unstemmed Characterisation of autophagy disruption in the ileum of pigs infected with Lawsonia intracellularis
title_short Characterisation of autophagy disruption in the ileum of pigs infected with Lawsonia intracellularis
title_sort characterisation of autophagy disruption in the ileum of pigs infected with lawsonia intracellularis
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9165227/
https://www.ncbi.nlm.nih.gov/pubmed/34669106
http://dx.doi.org/10.1007/s11259-021-09847-7
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