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In Vitro Hepatoprotective and Human Gut Microbiota Modulation of Polysaccharide-Peptides in Pleurotus citrinopileatus

Pleurotus citrinopileatus, a golden oyster mushroom, is popular in Asia and has pharmacological functions. However, the effects of polysaccharide-peptides extracted from Pleurotus citrinopileatus and underlying mechanism on digestive systme have not yet been clarified. Here, we determined the compos...

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Autores principales: Huang, Yihua, Gao, Yi, Pi, Xionge, Zhao, Shuang, Liu, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9165600/
https://www.ncbi.nlm.nih.gov/pubmed/35669115
http://dx.doi.org/10.3389/fcimb.2022.892049
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author Huang, Yihua
Gao, Yi
Pi, Xionge
Zhao, Shuang
Liu, Wei
author_facet Huang, Yihua
Gao, Yi
Pi, Xionge
Zhao, Shuang
Liu, Wei
author_sort Huang, Yihua
collection PubMed
description Pleurotus citrinopileatus, a golden oyster mushroom, is popular in Asia and has pharmacological functions. However, the effects of polysaccharide-peptides extracted from Pleurotus citrinopileatus and underlying mechanism on digestive systme have not yet been clarified. Here, we determined the composition of two polysaccharide-peptides (PSI and PSII) from P. citrinopileatus and investigated the protective effects of on hepatoprotective and gut microbiota. The results showed that PSI and PSII were made up of similar monosaccharide moieties, except for the varying ratios. Furthermore, PSI and PSII showed that they have the hepatoprotective effects and significantly increased the viabilities and cellular total superoxide dismutase activities increased significantly in HepG2 cells. Intracellular triglyceride content and extracellular alanine aminotransferase and aspartate transaminase contents markedly decreased following treatment with 40 and 50 μg/mL PSI and PSII, respectively. Moreover, PSI and PSII activated the adiponectin pathway and reduced lipid accumulation in liver cells. PSI and PSII elevated short-chain fatty acid concentrations, especially butyric and acetic acids. 16S rRNA gene sequencing analysis showed that PSI promoted the relative abundances of Bifidobacteria, Lactobacillus, Faecalibacterium, as well as Prevotella generas in the gut. PSII markedly suppressed the relative abundances of Escherichia-Shigella and Bacteroides generas. We speculate that the PSI and PSII play a role through liver-gut axis system. Polysaccharide-peptides metabolize by gut microbiota to produce short-chain fatty acids (SCFAs) and in turn influence liver functions.
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spelling pubmed-91656002022-06-05 In Vitro Hepatoprotective and Human Gut Microbiota Modulation of Polysaccharide-Peptides in Pleurotus citrinopileatus Huang, Yihua Gao, Yi Pi, Xionge Zhao, Shuang Liu, Wei Front Cell Infect Microbiol Cellular and Infection Microbiology Pleurotus citrinopileatus, a golden oyster mushroom, is popular in Asia and has pharmacological functions. However, the effects of polysaccharide-peptides extracted from Pleurotus citrinopileatus and underlying mechanism on digestive systme have not yet been clarified. Here, we determined the composition of two polysaccharide-peptides (PSI and PSII) from P. citrinopileatus and investigated the protective effects of on hepatoprotective and gut microbiota. The results showed that PSI and PSII were made up of similar monosaccharide moieties, except for the varying ratios. Furthermore, PSI and PSII showed that they have the hepatoprotective effects and significantly increased the viabilities and cellular total superoxide dismutase activities increased significantly in HepG2 cells. Intracellular triglyceride content and extracellular alanine aminotransferase and aspartate transaminase contents markedly decreased following treatment with 40 and 50 μg/mL PSI and PSII, respectively. Moreover, PSI and PSII activated the adiponectin pathway and reduced lipid accumulation in liver cells. PSI and PSII elevated short-chain fatty acid concentrations, especially butyric and acetic acids. 16S rRNA gene sequencing analysis showed that PSI promoted the relative abundances of Bifidobacteria, Lactobacillus, Faecalibacterium, as well as Prevotella generas in the gut. PSII markedly suppressed the relative abundances of Escherichia-Shigella and Bacteroides generas. We speculate that the PSI and PSII play a role through liver-gut axis system. Polysaccharide-peptides metabolize by gut microbiota to produce short-chain fatty acids (SCFAs) and in turn influence liver functions. Frontiers Media S.A. 2022-05-20 /pmc/articles/PMC9165600/ /pubmed/35669115 http://dx.doi.org/10.3389/fcimb.2022.892049 Text en Copyright © 2022 Huang, Gao, Pi, Zhao and Liu https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Huang, Yihua
Gao, Yi
Pi, Xionge
Zhao, Shuang
Liu, Wei
In Vitro Hepatoprotective and Human Gut Microbiota Modulation of Polysaccharide-Peptides in Pleurotus citrinopileatus
title In Vitro Hepatoprotective and Human Gut Microbiota Modulation of Polysaccharide-Peptides in Pleurotus citrinopileatus
title_full In Vitro Hepatoprotective and Human Gut Microbiota Modulation of Polysaccharide-Peptides in Pleurotus citrinopileatus
title_fullStr In Vitro Hepatoprotective and Human Gut Microbiota Modulation of Polysaccharide-Peptides in Pleurotus citrinopileatus
title_full_unstemmed In Vitro Hepatoprotective and Human Gut Microbiota Modulation of Polysaccharide-Peptides in Pleurotus citrinopileatus
title_short In Vitro Hepatoprotective and Human Gut Microbiota Modulation of Polysaccharide-Peptides in Pleurotus citrinopileatus
title_sort in vitro hepatoprotective and human gut microbiota modulation of polysaccharide-peptides in pleurotus citrinopileatus
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9165600/
https://www.ncbi.nlm.nih.gov/pubmed/35669115
http://dx.doi.org/10.3389/fcimb.2022.892049
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