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Occurrence and characterization of methicillin-resistant Staphylococcus spp. in diseased dogs in Brazil

Staphylococcus pseudintermedius is a major commensal bacterium of the skin and mucosae of dogs and an opportunistic agent responsible for several clinical infections, such as pyoderma, otitis, and surgical wound infections. The emergence of methicillin-resistant S. pseudintermedius (MRSP) has become...

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Autores principales: Viegas, Flávia Mello, Santana, Jordana Almeida, Silva, Brendhal Almeida, Xavier, Rafael Gariglio Clark, Bonisson, Cláudia Teixeira, Câmara, Júlia Lara Sette, Rennó, Mário Cesar, Cunha, João Luis Reis, Figueiredo, Henrique César Pereira, Lobato, Francisco Carlos Faria, Silva, Rodrigo Otávio Silveira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9165789/
https://www.ncbi.nlm.nih.gov/pubmed/35657980
http://dx.doi.org/10.1371/journal.pone.0269422
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author Viegas, Flávia Mello
Santana, Jordana Almeida
Silva, Brendhal Almeida
Xavier, Rafael Gariglio Clark
Bonisson, Cláudia Teixeira
Câmara, Júlia Lara Sette
Rennó, Mário Cesar
Cunha, João Luis Reis
Figueiredo, Henrique César Pereira
Lobato, Francisco Carlos Faria
Silva, Rodrigo Otávio Silveira
author_facet Viegas, Flávia Mello
Santana, Jordana Almeida
Silva, Brendhal Almeida
Xavier, Rafael Gariglio Clark
Bonisson, Cláudia Teixeira
Câmara, Júlia Lara Sette
Rennó, Mário Cesar
Cunha, João Luis Reis
Figueiredo, Henrique César Pereira
Lobato, Francisco Carlos Faria
Silva, Rodrigo Otávio Silveira
author_sort Viegas, Flávia Mello
collection PubMed
description Staphylococcus pseudintermedius is a major commensal bacterium of the skin and mucosae of dogs and an opportunistic agent responsible for several clinical infections, such as pyoderma, otitis, and surgical wound infections. The emergence of methicillin-resistant S. pseudintermedius (MRSP) has become a problem of great concern in veterinary and human medicine because it is multidrug resistant (MDR) and can also infect humans. This study aimed to identify the occurrence of Staphylococcus spp. in infected patients and investigate the antimicrobial resistance profiles and molecular structure of MRSP isolates. Samples were obtained from two different veterinary clinics; suggestive colonies were submitted to matrix-assisted laser desorption ionization-time of flight (MALDI-ToF) mass spectrometry and confirmed at the species level by polymerase chain reaction (PCR). Sequencing of the 16S rRNA and rpoB genes were used in selected samples that were not identified by MALDI-ToF and by the species-specific PCR. Antimicrobial susceptibility and PCR detection of mecA were performed. MRSP isolates were subjected to multilocus sequence typing. Of all the clinical staphylococci (n = 131), 98 (74.8%) were identified as S. pseudintermedius. Multidrug resistance (resistance to ≥3 classes of antimicrobials) was observed in 63.2% of S. pseudintermedius isolates, and 24.5% of S. pseudintermedius isolates were methicillin-resistant. Half of the MRSP isolates were isolated from surgical site infections. Among the ten sequence types (ST) identified, nine were novel. ST71 was the most prevalent and associated with resistance to fluoroquinolones. Prior antimicrobial therapy, hospitalization, and surgical site infections seemed to be risk factors for MRSP acquisition. The present study showed a high rate of MDR staphylococci in infected dogs. MRSP was isolated from different clinical conditions, mainly surgical site infections. Additionally, this is the first study to extensively investigate the population structure of MRSP in Brazil, which revealed the dispersion of CC71 and nine novel ST. These findings raise concerns for both animal and human health due to the zoonotic potential of this species and limited therapeutic options available for MRSP infections.
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spelling pubmed-91657892022-06-05 Occurrence and characterization of methicillin-resistant Staphylococcus spp. in diseased dogs in Brazil Viegas, Flávia Mello Santana, Jordana Almeida Silva, Brendhal Almeida Xavier, Rafael Gariglio Clark Bonisson, Cláudia Teixeira Câmara, Júlia Lara Sette Rennó, Mário Cesar Cunha, João Luis Reis Figueiredo, Henrique César Pereira Lobato, Francisco Carlos Faria Silva, Rodrigo Otávio Silveira PLoS One Research Article Staphylococcus pseudintermedius is a major commensal bacterium of the skin and mucosae of dogs and an opportunistic agent responsible for several clinical infections, such as pyoderma, otitis, and surgical wound infections. The emergence of methicillin-resistant S. pseudintermedius (MRSP) has become a problem of great concern in veterinary and human medicine because it is multidrug resistant (MDR) and can also infect humans. This study aimed to identify the occurrence of Staphylococcus spp. in infected patients and investigate the antimicrobial resistance profiles and molecular structure of MRSP isolates. Samples were obtained from two different veterinary clinics; suggestive colonies were submitted to matrix-assisted laser desorption ionization-time of flight (MALDI-ToF) mass spectrometry and confirmed at the species level by polymerase chain reaction (PCR). Sequencing of the 16S rRNA and rpoB genes were used in selected samples that were not identified by MALDI-ToF and by the species-specific PCR. Antimicrobial susceptibility and PCR detection of mecA were performed. MRSP isolates were subjected to multilocus sequence typing. Of all the clinical staphylococci (n = 131), 98 (74.8%) were identified as S. pseudintermedius. Multidrug resistance (resistance to ≥3 classes of antimicrobials) was observed in 63.2% of S. pseudintermedius isolates, and 24.5% of S. pseudintermedius isolates were methicillin-resistant. Half of the MRSP isolates were isolated from surgical site infections. Among the ten sequence types (ST) identified, nine were novel. ST71 was the most prevalent and associated with resistance to fluoroquinolones. Prior antimicrobial therapy, hospitalization, and surgical site infections seemed to be risk factors for MRSP acquisition. The present study showed a high rate of MDR staphylococci in infected dogs. MRSP was isolated from different clinical conditions, mainly surgical site infections. Additionally, this is the first study to extensively investigate the population structure of MRSP in Brazil, which revealed the dispersion of CC71 and nine novel ST. These findings raise concerns for both animal and human health due to the zoonotic potential of this species and limited therapeutic options available for MRSP infections. Public Library of Science 2022-06-03 /pmc/articles/PMC9165789/ /pubmed/35657980 http://dx.doi.org/10.1371/journal.pone.0269422 Text en © 2022 Viegas et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Viegas, Flávia Mello
Santana, Jordana Almeida
Silva, Brendhal Almeida
Xavier, Rafael Gariglio Clark
Bonisson, Cláudia Teixeira
Câmara, Júlia Lara Sette
Rennó, Mário Cesar
Cunha, João Luis Reis
Figueiredo, Henrique César Pereira
Lobato, Francisco Carlos Faria
Silva, Rodrigo Otávio Silveira
Occurrence and characterization of methicillin-resistant Staphylococcus spp. in diseased dogs in Brazil
title Occurrence and characterization of methicillin-resistant Staphylococcus spp. in diseased dogs in Brazil
title_full Occurrence and characterization of methicillin-resistant Staphylococcus spp. in diseased dogs in Brazil
title_fullStr Occurrence and characterization of methicillin-resistant Staphylococcus spp. in diseased dogs in Brazil
title_full_unstemmed Occurrence and characterization of methicillin-resistant Staphylococcus spp. in diseased dogs in Brazil
title_short Occurrence and characterization of methicillin-resistant Staphylococcus spp. in diseased dogs in Brazil
title_sort occurrence and characterization of methicillin-resistant staphylococcus spp. in diseased dogs in brazil
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9165789/
https://www.ncbi.nlm.nih.gov/pubmed/35657980
http://dx.doi.org/10.1371/journal.pone.0269422
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