Increased LAMP1 Expression Enhances SARS-CoV-1 and SARS-CoV-2 Production in Vero-Derived Transgenic Cell Lines

Coronaviridae is a family of single-stranded RNA (ssRNA) viruses that can cause diseases with high mortality rates. SARS-CoV-1 and MERS-CoV appeared in 2002‒2003 and 2012, respectively. A novel coronavirus, SARS-CoV-2, emerged in 2019 in Wuhan (China) and has caused more than 5 million deaths in wor...

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Autores principales: Dolskiy, A. A., Grishchenko, I. V., Bodnev, S. A., Nazarenko, A. A., Smirnova, A. M., Matveeva, A. K., Bulychev, L. E., Ovchinnikova, A. S., Tregubchak, T. V., Zaykovskaya, A. V., Imatdinov, I. R., Pyankov, O. V., Gavrilova, E. V., Maksyutov, R. A., Yudkin, D. V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Pleiades Publishing 2022
Materias:
Molecular Cell Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9165926/
https://www.ncbi.nlm.nih.gov/pubmed/35693978
http://dx.doi.org/10.1134/S0026893322030050
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author Dolskiy, A. A.
Grishchenko, I. V.
Bodnev, S. A.
Nazarenko, A. A.
Smirnova, A. M.
Matveeva, A. K.
Bulychev, L. E.
Ovchinnikova, A. S.
Tregubchak, T. V.
Zaykovskaya, A. V.
Imatdinov, I. R.
Pyankov, O. V.
Gavrilova, E. V.
Maksyutov, R. A.
Yudkin, D. V.
author_facet Dolskiy, A. A.
Grishchenko, I. V.
Bodnev, S. A.
Nazarenko, A. A.
Smirnova, A. M.
Matveeva, A. K.
Bulychev, L. E.
Ovchinnikova, A. S.
Tregubchak, T. V.
Zaykovskaya, A. V.
Imatdinov, I. R.
Pyankov, O. V.
Gavrilova, E. V.
Maksyutov, R. A.
Yudkin, D. V.
author_sort Dolskiy, A. A.
collection PubMed
description Coronaviridae is a family of single-stranded RNA (ssRNA) viruses that can cause diseases with high mortality rates. SARS-CoV-1 and MERS-CoV appeared in 2002‒2003 and 2012, respectively. A novel coronavirus, SARS-CoV-2, emerged in 2019 in Wuhan (China) and has caused more than 5 million deaths in worldwide. The entry of SARS-CoV-1 into the cell is due to the interaction of the viral spike (S) protein and the cell protein, angiotensin-converting enzyme 2 (ACE2). After infection, virus assembly occurs in Golgi apparatus-derived vesicles during exocytosis. One of the possible participants in this process is LAMP1 protein. We established transgenic Vero cell lines with increased expression of human LAMP1 gene and evaluated SARS-CoV-1 and SARS-CoV-2 production. An increase in the production of both viruses in LAMP1-expressing cells when compared with Vero cells was observed, especially in the presence of trypsin during infection. From these results it can be assumed that LAMP1 promotes SARS-CoV-1 and SARS-CoV-2 production due to enhanced exocytosis.
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spelling pubmed-91659262022-06-07 Increased LAMP1 Expression Enhances SARS-CoV-1 and SARS-CoV-2 Production in Vero-Derived Transgenic Cell Lines Dolskiy, A. A. Grishchenko, I. V. Bodnev, S. A. Nazarenko, A. A. Smirnova, A. M. Matveeva, A. K. Bulychev, L. E. Ovchinnikova, A. S. Tregubchak, T. V. Zaykovskaya, A. V. Imatdinov, I. R. Pyankov, O. V. Gavrilova, E. V. Maksyutov, R. A. Yudkin, D. V. Mol Biol Molecular Cell Biology Coronaviridae is a family of single-stranded RNA (ssRNA) viruses that can cause diseases with high mortality rates. SARS-CoV-1 and MERS-CoV appeared in 2002‒2003 and 2012, respectively. A novel coronavirus, SARS-CoV-2, emerged in 2019 in Wuhan (China) and has caused more than 5 million deaths in worldwide. The entry of SARS-CoV-1 into the cell is due to the interaction of the viral spike (S) protein and the cell protein, angiotensin-converting enzyme 2 (ACE2). After infection, virus assembly occurs in Golgi apparatus-derived vesicles during exocytosis. One of the possible participants in this process is LAMP1 protein. We established transgenic Vero cell lines with increased expression of human LAMP1 gene and evaluated SARS-CoV-1 and SARS-CoV-2 production. An increase in the production of both viruses in LAMP1-expressing cells when compared with Vero cells was observed, especially in the presence of trypsin during infection. From these results it can be assumed that LAMP1 promotes SARS-CoV-1 and SARS-CoV-2 production due to enhanced exocytosis. Pleiades Publishing 2022-06-03 2022 /pmc/articles/PMC9165926/ /pubmed/35693978 http://dx.doi.org/10.1134/S0026893322030050 Text en © Pleiades Publishing, Inc. 2022, ISSN 0026-8933, Molecular Biology, 2022, Vol. 56, No. 3, pp. 463–468. © Pleiades Publishing, Inc., 2022.Russian Text © The Author(s), 2022, published in Molekulyarnaya Biologiya, 2022, Vol. 56, No. 3, pp. 503–509. This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Molecular Cell Biology
Dolskiy, A. A.
Grishchenko, I. V.
Bodnev, S. A.
Nazarenko, A. A.
Smirnova, A. M.
Matveeva, A. K.
Bulychev, L. E.
Ovchinnikova, A. S.
Tregubchak, T. V.
Zaykovskaya, A. V.
Imatdinov, I. R.
Pyankov, O. V.
Gavrilova, E. V.
Maksyutov, R. A.
Yudkin, D. V.
Increased LAMP1 Expression Enhances SARS-CoV-1 and SARS-CoV-2 Production in Vero-Derived Transgenic Cell Lines
title Increased LAMP1 Expression Enhances SARS-CoV-1 and SARS-CoV-2 Production in Vero-Derived Transgenic Cell Lines
title_full Increased LAMP1 Expression Enhances SARS-CoV-1 and SARS-CoV-2 Production in Vero-Derived Transgenic Cell Lines
title_fullStr Increased LAMP1 Expression Enhances SARS-CoV-1 and SARS-CoV-2 Production in Vero-Derived Transgenic Cell Lines
title_full_unstemmed Increased LAMP1 Expression Enhances SARS-CoV-1 and SARS-CoV-2 Production in Vero-Derived Transgenic Cell Lines
title_short Increased LAMP1 Expression Enhances SARS-CoV-1 and SARS-CoV-2 Production in Vero-Derived Transgenic Cell Lines
title_sort increased lamp1 expression enhances sars-cov-1 and sars-cov-2 production in vero-derived transgenic cell lines
topic Molecular Cell Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9165926/
https://www.ncbi.nlm.nih.gov/pubmed/35693978
http://dx.doi.org/10.1134/S0026893322030050
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