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Microfluidic particle dam for direct visualization of SARS-CoV-2 antibody levels in COVID-19 vaccinees

Various COVID-19 vaccines are currently deployed, but their immunization varies and decays with time. Antibody level is a potent correlate to immune protection, but its quantitation relies on intensive laboratory techniques. Here, we report a decentralized, instrument-free microfluidic device that d...

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Autores principales: Wu, Minghui, Wu, Siying, Wang, Gaobo, Liu, Wengang, Chu, Lok Ting, Jiang, Tianyi, Kwong, Hoi Kwan, Chow, Hiu Lam, Li, Iris Wai Sum, Chen, Ting-Hsuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9166397/
https://www.ncbi.nlm.nih.gov/pubmed/35658040
http://dx.doi.org/10.1126/sciadv.abn6064
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author Wu, Minghui
Wu, Siying
Wang, Gaobo
Liu, Wengang
Chu, Lok Ting
Jiang, Tianyi
Kwong, Hoi Kwan
Chow, Hiu Lam
Li, Iris Wai Sum
Chen, Ting-Hsuan
author_facet Wu, Minghui
Wu, Siying
Wang, Gaobo
Liu, Wengang
Chu, Lok Ting
Jiang, Tianyi
Kwong, Hoi Kwan
Chow, Hiu Lam
Li, Iris Wai Sum
Chen, Ting-Hsuan
author_sort Wu, Minghui
collection PubMed
description Various COVID-19 vaccines are currently deployed, but their immunization varies and decays with time. Antibody level is a potent correlate to immune protection, but its quantitation relies on intensive laboratory techniques. Here, we report a decentralized, instrument-free microfluidic device that directly visualizes SARS-CoV-2 antibody levels. Magnetic microparticles (MMPs) and polystyrene microparticles (PMPs) can bind to SARS-CoV-2 antibodies simultaneously. In a microfluidic chip, this binding reduces the incidence of free PMPs escaping from magnetic separation and shortens PMP accumulation length at a particle dam. This visual quantitative result enables use in either sensitive mode [limit of detection (LOD): 13.3 ng/ml; sample-to-answer time: 70 min] or rapid mode (LOD: 57.8 ng/ml; sample-to-answer time: 20 min) and closely agrees with the gold standard enzyme-linked immunosorbent assay. Trials on 91 vaccinees revealed higher antibody levels in mRNA vaccinees than in inactivated vaccinees and their decay in 45 days, demonstrating the need for point-of-care devices to monitor immune protection.
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spelling pubmed-91663972022-06-17 Microfluidic particle dam for direct visualization of SARS-CoV-2 antibody levels in COVID-19 vaccinees Wu, Minghui Wu, Siying Wang, Gaobo Liu, Wengang Chu, Lok Ting Jiang, Tianyi Kwong, Hoi Kwan Chow, Hiu Lam Li, Iris Wai Sum Chen, Ting-Hsuan Sci Adv Biomedicine and Life Sciences Various COVID-19 vaccines are currently deployed, but their immunization varies and decays with time. Antibody level is a potent correlate to immune protection, but its quantitation relies on intensive laboratory techniques. Here, we report a decentralized, instrument-free microfluidic device that directly visualizes SARS-CoV-2 antibody levels. Magnetic microparticles (MMPs) and polystyrene microparticles (PMPs) can bind to SARS-CoV-2 antibodies simultaneously. In a microfluidic chip, this binding reduces the incidence of free PMPs escaping from magnetic separation and shortens PMP accumulation length at a particle dam. This visual quantitative result enables use in either sensitive mode [limit of detection (LOD): 13.3 ng/ml; sample-to-answer time: 70 min] or rapid mode (LOD: 57.8 ng/ml; sample-to-answer time: 20 min) and closely agrees with the gold standard enzyme-linked immunosorbent assay. Trials on 91 vaccinees revealed higher antibody levels in mRNA vaccinees than in inactivated vaccinees and their decay in 45 days, demonstrating the need for point-of-care devices to monitor immune protection. American Association for the Advancement of Science 2022-06-03 /pmc/articles/PMC9166397/ /pubmed/35658040 http://dx.doi.org/10.1126/sciadv.abn6064 Text en Copyright © 2022 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY). https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution license (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Biomedicine and Life Sciences
Wu, Minghui
Wu, Siying
Wang, Gaobo
Liu, Wengang
Chu, Lok Ting
Jiang, Tianyi
Kwong, Hoi Kwan
Chow, Hiu Lam
Li, Iris Wai Sum
Chen, Ting-Hsuan
Microfluidic particle dam for direct visualization of SARS-CoV-2 antibody levels in COVID-19 vaccinees
title Microfluidic particle dam for direct visualization of SARS-CoV-2 antibody levels in COVID-19 vaccinees
title_full Microfluidic particle dam for direct visualization of SARS-CoV-2 antibody levels in COVID-19 vaccinees
title_fullStr Microfluidic particle dam for direct visualization of SARS-CoV-2 antibody levels in COVID-19 vaccinees
title_full_unstemmed Microfluidic particle dam for direct visualization of SARS-CoV-2 antibody levels in COVID-19 vaccinees
title_short Microfluidic particle dam for direct visualization of SARS-CoV-2 antibody levels in COVID-19 vaccinees
title_sort microfluidic particle dam for direct visualization of sars-cov-2 antibody levels in covid-19 vaccinees
topic Biomedicine and Life Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9166397/
https://www.ncbi.nlm.nih.gov/pubmed/35658040
http://dx.doi.org/10.1126/sciadv.abn6064
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