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A method to correct for the influence of bovine serum albumin-associated vitamin D metabolites in protein extracts from neonatal dried blood spots
OBJECTIVE: We developed an assay to measure the concentration of 25 hydroxyvitamin D(2) and D(3) in protein extracts derived from stored neonatal dried blood spots. During this study, we postulated that these samples had been contaminated with exogenous vitamin D metabolites because of the addition...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9166528/ https://www.ncbi.nlm.nih.gov/pubmed/35659347 http://dx.doi.org/10.1186/s13104-022-06077-1 |
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author | Boelt, Sanne Grundvad Plana-Ripoll, Oleguer Albiñana, Clara Vilhjálmsson, Bjarni McGrath, John J. Cohen, Arieh S. |
author_facet | Boelt, Sanne Grundvad Plana-Ripoll, Oleguer Albiñana, Clara Vilhjálmsson, Bjarni McGrath, John J. Cohen, Arieh S. |
author_sort | Boelt, Sanne Grundvad |
collection | PubMed |
description | OBJECTIVE: We developed an assay to measure the concentration of 25 hydroxyvitamin D(2) and D(3) in protein extracts derived from stored neonatal dried blood spots. During this study, we postulated that these samples had been contaminated with exogenous vitamin D metabolites because of the addition of bovine serum albumin (BSA) as part of an extraction step undertaken 7 years earlier. The aim of the current study was to develop methods in order to adjust for this contamination. RESULTS: We identified between-plate variations in 25 hydroxyvitamin D(2) and D(3) concentrations which suggested the presence of three different BSA batches. Based on repeat extraction (without the addition of BSA) and testing of 395 samples, we developed models to correct for the exogenous 25 hydroxyvitamin D(2) and D(3.) The regression models were Diff(25OHD3) = − 8.2 + 1.8* Diff(25OHD2) for low contamination, Diff(25OHD3) = 23.8 + 1.7* Diff(25OHD2) for middle contamination, and Diff(25OHD3) = 14.3 + 3.0* Diff(25OHD2) for high contamination. After these corrections, the three subsamples had comparable distributions within the expected range for both 25 hydroxyvitamin D(2) and D(3). SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13104-022-06077-1. |
format | Online Article Text |
id | pubmed-9166528 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-91665282022-06-05 A method to correct for the influence of bovine serum albumin-associated vitamin D metabolites in protein extracts from neonatal dried blood spots Boelt, Sanne Grundvad Plana-Ripoll, Oleguer Albiñana, Clara Vilhjálmsson, Bjarni McGrath, John J. Cohen, Arieh S. BMC Res Notes Research Note OBJECTIVE: We developed an assay to measure the concentration of 25 hydroxyvitamin D(2) and D(3) in protein extracts derived from stored neonatal dried blood spots. During this study, we postulated that these samples had been contaminated with exogenous vitamin D metabolites because of the addition of bovine serum albumin (BSA) as part of an extraction step undertaken 7 years earlier. The aim of the current study was to develop methods in order to adjust for this contamination. RESULTS: We identified between-plate variations in 25 hydroxyvitamin D(2) and D(3) concentrations which suggested the presence of three different BSA batches. Based on repeat extraction (without the addition of BSA) and testing of 395 samples, we developed models to correct for the exogenous 25 hydroxyvitamin D(2) and D(3.) The regression models were Diff(25OHD3) = − 8.2 + 1.8* Diff(25OHD2) for low contamination, Diff(25OHD3) = 23.8 + 1.7* Diff(25OHD2) for middle contamination, and Diff(25OHD3) = 14.3 + 3.0* Diff(25OHD2) for high contamination. After these corrections, the three subsamples had comparable distributions within the expected range for both 25 hydroxyvitamin D(2) and D(3). SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13104-022-06077-1. BioMed Central 2022-06-03 /pmc/articles/PMC9166528/ /pubmed/35659347 http://dx.doi.org/10.1186/s13104-022-06077-1 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Note Boelt, Sanne Grundvad Plana-Ripoll, Oleguer Albiñana, Clara Vilhjálmsson, Bjarni McGrath, John J. Cohen, Arieh S. A method to correct for the influence of bovine serum albumin-associated vitamin D metabolites in protein extracts from neonatal dried blood spots |
title | A method to correct for the influence of bovine serum albumin-associated vitamin D metabolites in protein extracts from neonatal dried blood spots |
title_full | A method to correct for the influence of bovine serum albumin-associated vitamin D metabolites in protein extracts from neonatal dried blood spots |
title_fullStr | A method to correct for the influence of bovine serum albumin-associated vitamin D metabolites in protein extracts from neonatal dried blood spots |
title_full_unstemmed | A method to correct for the influence of bovine serum albumin-associated vitamin D metabolites in protein extracts from neonatal dried blood spots |
title_short | A method to correct for the influence of bovine serum albumin-associated vitamin D metabolites in protein extracts from neonatal dried blood spots |
title_sort | method to correct for the influence of bovine serum albumin-associated vitamin d metabolites in protein extracts from neonatal dried blood spots |
topic | Research Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9166528/ https://www.ncbi.nlm.nih.gov/pubmed/35659347 http://dx.doi.org/10.1186/s13104-022-06077-1 |
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