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Callose deposited at soybean sieve element inhibits long-distance transport of Soybean mosaic virus

The function of callose and its deposition characteristics at phloem in the resistance to the long-distance transportation of Soybean mosaic virus (SMV) through phloem was studied. Two different methods of SMV inoculation were used in the study, one was direct friction of the virus on seedling leave...

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Autores principales: Zhang, Jie, Liu, Na, Yan, Aihua, Sun, Tianjie, Sun, Xizhe, Yao, Guibin, Xiao, Dongqiang, Li, Wenlong, Hou, Chunyan, Yang, Chunyan, Wang, Dongmei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9167352/
https://www.ncbi.nlm.nih.gov/pubmed/35660979
http://dx.doi.org/10.1186/s13568-022-01402-0
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author Zhang, Jie
Liu, Na
Yan, Aihua
Sun, Tianjie
Sun, Xizhe
Yao, Guibin
Xiao, Dongqiang
Li, Wenlong
Hou, Chunyan
Yang, Chunyan
Wang, Dongmei
author_facet Zhang, Jie
Liu, Na
Yan, Aihua
Sun, Tianjie
Sun, Xizhe
Yao, Guibin
Xiao, Dongqiang
Li, Wenlong
Hou, Chunyan
Yang, Chunyan
Wang, Dongmei
author_sort Zhang, Jie
collection PubMed
description The function of callose and its deposition characteristics at phloem in the resistance to the long-distance transportation of Soybean mosaic virus (SMV) through phloem was studied. Two different methods of SMV inoculation were used in the study, one was direct friction of the virus on seedling leaves and the other was based on grafting scion and rootstock to create different resistance and sensitivity combinations. Veins, petioles of inoculated leaves and rootstock stems were stained with callose specific dye. Results from fluorescence microscope observation, pharmacological test, and PCR detection of SMV coat protein gene (SMV-CP) showed the role of callose in long-distance transportation of SMV through phloem during infection of soybean seedlings. When the inhibitor of callose synthesis 2-deoxy-D-glucose (2-DDG) was used, the accumulation of callose fluorescence could hardly be detected in the resistant rootstocks. These results indicate that callose deposition in phloem restricts the long-distance transport of SMV, and that the accumulation of callose in phloem is a main contributing factor for resistance to this virus in soybean.
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spelling pubmed-91673522022-06-06 Callose deposited at soybean sieve element inhibits long-distance transport of Soybean mosaic virus Zhang, Jie Liu, Na Yan, Aihua Sun, Tianjie Sun, Xizhe Yao, Guibin Xiao, Dongqiang Li, Wenlong Hou, Chunyan Yang, Chunyan Wang, Dongmei AMB Express Original Article The function of callose and its deposition characteristics at phloem in the resistance to the long-distance transportation of Soybean mosaic virus (SMV) through phloem was studied. Two different methods of SMV inoculation were used in the study, one was direct friction of the virus on seedling leaves and the other was based on grafting scion and rootstock to create different resistance and sensitivity combinations. Veins, petioles of inoculated leaves and rootstock stems were stained with callose specific dye. Results from fluorescence microscope observation, pharmacological test, and PCR detection of SMV coat protein gene (SMV-CP) showed the role of callose in long-distance transportation of SMV through phloem during infection of soybean seedlings. When the inhibitor of callose synthesis 2-deoxy-D-glucose (2-DDG) was used, the accumulation of callose fluorescence could hardly be detected in the resistant rootstocks. These results indicate that callose deposition in phloem restricts the long-distance transport of SMV, and that the accumulation of callose in phloem is a main contributing factor for resistance to this virus in soybean. Springer Berlin Heidelberg 2022-06-04 /pmc/articles/PMC9167352/ /pubmed/35660979 http://dx.doi.org/10.1186/s13568-022-01402-0 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Article
Zhang, Jie
Liu, Na
Yan, Aihua
Sun, Tianjie
Sun, Xizhe
Yao, Guibin
Xiao, Dongqiang
Li, Wenlong
Hou, Chunyan
Yang, Chunyan
Wang, Dongmei
Callose deposited at soybean sieve element inhibits long-distance transport of Soybean mosaic virus
title Callose deposited at soybean sieve element inhibits long-distance transport of Soybean mosaic virus
title_full Callose deposited at soybean sieve element inhibits long-distance transport of Soybean mosaic virus
title_fullStr Callose deposited at soybean sieve element inhibits long-distance transport of Soybean mosaic virus
title_full_unstemmed Callose deposited at soybean sieve element inhibits long-distance transport of Soybean mosaic virus
title_short Callose deposited at soybean sieve element inhibits long-distance transport of Soybean mosaic virus
title_sort callose deposited at soybean sieve element inhibits long-distance transport of soybean mosaic virus
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9167352/
https://www.ncbi.nlm.nih.gov/pubmed/35660979
http://dx.doi.org/10.1186/s13568-022-01402-0
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