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Callose deposited at soybean sieve element inhibits long-distance transport of Soybean mosaic virus
The function of callose and its deposition characteristics at phloem in the resistance to the long-distance transportation of Soybean mosaic virus (SMV) through phloem was studied. Two different methods of SMV inoculation were used in the study, one was direct friction of the virus on seedling leave...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9167352/ https://www.ncbi.nlm.nih.gov/pubmed/35660979 http://dx.doi.org/10.1186/s13568-022-01402-0 |
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author | Zhang, Jie Liu, Na Yan, Aihua Sun, Tianjie Sun, Xizhe Yao, Guibin Xiao, Dongqiang Li, Wenlong Hou, Chunyan Yang, Chunyan Wang, Dongmei |
author_facet | Zhang, Jie Liu, Na Yan, Aihua Sun, Tianjie Sun, Xizhe Yao, Guibin Xiao, Dongqiang Li, Wenlong Hou, Chunyan Yang, Chunyan Wang, Dongmei |
author_sort | Zhang, Jie |
collection | PubMed |
description | The function of callose and its deposition characteristics at phloem in the resistance to the long-distance transportation of Soybean mosaic virus (SMV) through phloem was studied. Two different methods of SMV inoculation were used in the study, one was direct friction of the virus on seedling leaves and the other was based on grafting scion and rootstock to create different resistance and sensitivity combinations. Veins, petioles of inoculated leaves and rootstock stems were stained with callose specific dye. Results from fluorescence microscope observation, pharmacological test, and PCR detection of SMV coat protein gene (SMV-CP) showed the role of callose in long-distance transportation of SMV through phloem during infection of soybean seedlings. When the inhibitor of callose synthesis 2-deoxy-D-glucose (2-DDG) was used, the accumulation of callose fluorescence could hardly be detected in the resistant rootstocks. These results indicate that callose deposition in phloem restricts the long-distance transport of SMV, and that the accumulation of callose in phloem is a main contributing factor for resistance to this virus in soybean. |
format | Online Article Text |
id | pubmed-9167352 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-91673522022-06-06 Callose deposited at soybean sieve element inhibits long-distance transport of Soybean mosaic virus Zhang, Jie Liu, Na Yan, Aihua Sun, Tianjie Sun, Xizhe Yao, Guibin Xiao, Dongqiang Li, Wenlong Hou, Chunyan Yang, Chunyan Wang, Dongmei AMB Express Original Article The function of callose and its deposition characteristics at phloem in the resistance to the long-distance transportation of Soybean mosaic virus (SMV) through phloem was studied. Two different methods of SMV inoculation were used in the study, one was direct friction of the virus on seedling leaves and the other was based on grafting scion and rootstock to create different resistance and sensitivity combinations. Veins, petioles of inoculated leaves and rootstock stems were stained with callose specific dye. Results from fluorescence microscope observation, pharmacological test, and PCR detection of SMV coat protein gene (SMV-CP) showed the role of callose in long-distance transportation of SMV through phloem during infection of soybean seedlings. When the inhibitor of callose synthesis 2-deoxy-D-glucose (2-DDG) was used, the accumulation of callose fluorescence could hardly be detected in the resistant rootstocks. These results indicate that callose deposition in phloem restricts the long-distance transport of SMV, and that the accumulation of callose in phloem is a main contributing factor for resistance to this virus in soybean. Springer Berlin Heidelberg 2022-06-04 /pmc/articles/PMC9167352/ /pubmed/35660979 http://dx.doi.org/10.1186/s13568-022-01402-0 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Original Article Zhang, Jie Liu, Na Yan, Aihua Sun, Tianjie Sun, Xizhe Yao, Guibin Xiao, Dongqiang Li, Wenlong Hou, Chunyan Yang, Chunyan Wang, Dongmei Callose deposited at soybean sieve element inhibits long-distance transport of Soybean mosaic virus |
title | Callose deposited at soybean sieve element inhibits long-distance transport of Soybean mosaic virus |
title_full | Callose deposited at soybean sieve element inhibits long-distance transport of Soybean mosaic virus |
title_fullStr | Callose deposited at soybean sieve element inhibits long-distance transport of Soybean mosaic virus |
title_full_unstemmed | Callose deposited at soybean sieve element inhibits long-distance transport of Soybean mosaic virus |
title_short | Callose deposited at soybean sieve element inhibits long-distance transport of Soybean mosaic virus |
title_sort | callose deposited at soybean sieve element inhibits long-distance transport of soybean mosaic virus |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9167352/ https://www.ncbi.nlm.nih.gov/pubmed/35660979 http://dx.doi.org/10.1186/s13568-022-01402-0 |
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