Cargando…

Electromagnetically-driven integrated microfluidic platform using reverse transcription loop-mediated isothermal amplification for detection of severe acute respiratory syndrome coronavirus 2

Rapid, sensitive and accurate diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is of great need for effective quarantining and treatment. Real-time reverse-transcription polymerase chain reaction requiring thermocyling has been commonly used for diagnosis of SARS-CoV-2 thoug...

Descripción completa

Detalles Bibliográficos
Autores principales: Tsai, Yu-Shiuan, Wang, Chih-Hung, Tsai, Huey-Pin, Shan, Yan-Shen, Lee, Gwo-Bin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9167649/
https://www.ncbi.nlm.nih.gov/pubmed/35715135
http://dx.doi.org/10.1016/j.aca.2022.340036
_version_ 1784720844670369792
author Tsai, Yu-Shiuan
Wang, Chih-Hung
Tsai, Huey-Pin
Shan, Yan-Shen
Lee, Gwo-Bin
author_facet Tsai, Yu-Shiuan
Wang, Chih-Hung
Tsai, Huey-Pin
Shan, Yan-Shen
Lee, Gwo-Bin
author_sort Tsai, Yu-Shiuan
collection PubMed
description Rapid, sensitive and accurate diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is of great need for effective quarantining and treatment. Real-time reverse-transcription polymerase chain reaction requiring thermocyling has been commonly used for diagnosis of SARS-CoV-2 though it may take two to 4 h before lengthy sample pretreatment process and require bulky apparatus and well-trained personnel. Since multiple reverse transcription loop-mediated isothermal amplification (multiple RT-LAMP) process without thermocycling is sensitive, specific and fast, an electromagnetically-driven microfluidic chip (EMC) was developed herein to lyse SARS-CoV-2 viruses, extract their RNAs, and perform qualitative analysis of three marker genes by on-chip multiple RT-LAMP in an automatic format within 82 min at a limit of detection of only ∼5000 copies per reaction (i.e. 200 virus/ [Formula: see text]. This compact EMC may be especially promising for SARS-CoV-2 diagnostics in resource-limited countries.
format Online
Article
Text
id pubmed-9167649
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Elsevier B.V.
record_format MEDLINE/PubMed
spelling pubmed-91676492022-06-07 Electromagnetically-driven integrated microfluidic platform using reverse transcription loop-mediated isothermal amplification for detection of severe acute respiratory syndrome coronavirus 2 Tsai, Yu-Shiuan Wang, Chih-Hung Tsai, Huey-Pin Shan, Yan-Shen Lee, Gwo-Bin Anal Chim Acta Article Rapid, sensitive and accurate diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is of great need for effective quarantining and treatment. Real-time reverse-transcription polymerase chain reaction requiring thermocyling has been commonly used for diagnosis of SARS-CoV-2 though it may take two to 4 h before lengthy sample pretreatment process and require bulky apparatus and well-trained personnel. Since multiple reverse transcription loop-mediated isothermal amplification (multiple RT-LAMP) process without thermocycling is sensitive, specific and fast, an electromagnetically-driven microfluidic chip (EMC) was developed herein to lyse SARS-CoV-2 viruses, extract their RNAs, and perform qualitative analysis of three marker genes by on-chip multiple RT-LAMP in an automatic format within 82 min at a limit of detection of only ∼5000 copies per reaction (i.e. 200 virus/ [Formula: see text]. This compact EMC may be especially promising for SARS-CoV-2 diagnostics in resource-limited countries. Elsevier B.V. 2022-08-01 2022-06-06 /pmc/articles/PMC9167649/ /pubmed/35715135 http://dx.doi.org/10.1016/j.aca.2022.340036 Text en © 2022 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Tsai, Yu-Shiuan
Wang, Chih-Hung
Tsai, Huey-Pin
Shan, Yan-Shen
Lee, Gwo-Bin
Electromagnetically-driven integrated microfluidic platform using reverse transcription loop-mediated isothermal amplification for detection of severe acute respiratory syndrome coronavirus 2
title Electromagnetically-driven integrated microfluidic platform using reverse transcription loop-mediated isothermal amplification for detection of severe acute respiratory syndrome coronavirus 2
title_full Electromagnetically-driven integrated microfluidic platform using reverse transcription loop-mediated isothermal amplification for detection of severe acute respiratory syndrome coronavirus 2
title_fullStr Electromagnetically-driven integrated microfluidic platform using reverse transcription loop-mediated isothermal amplification for detection of severe acute respiratory syndrome coronavirus 2
title_full_unstemmed Electromagnetically-driven integrated microfluidic platform using reverse transcription loop-mediated isothermal amplification for detection of severe acute respiratory syndrome coronavirus 2
title_short Electromagnetically-driven integrated microfluidic platform using reverse transcription loop-mediated isothermal amplification for detection of severe acute respiratory syndrome coronavirus 2
title_sort electromagnetically-driven integrated microfluidic platform using reverse transcription loop-mediated isothermal amplification for detection of severe acute respiratory syndrome coronavirus 2
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9167649/
https://www.ncbi.nlm.nih.gov/pubmed/35715135
http://dx.doi.org/10.1016/j.aca.2022.340036
work_keys_str_mv AT tsaiyushiuan electromagneticallydrivenintegratedmicrofluidicplatformusingreversetranscriptionloopmediatedisothermalamplificationfordetectionofsevereacuterespiratorysyndromecoronavirus2
AT wangchihhung electromagneticallydrivenintegratedmicrofluidicplatformusingreversetranscriptionloopmediatedisothermalamplificationfordetectionofsevereacuterespiratorysyndromecoronavirus2
AT tsaihueypin electromagneticallydrivenintegratedmicrofluidicplatformusingreversetranscriptionloopmediatedisothermalamplificationfordetectionofsevereacuterespiratorysyndromecoronavirus2
AT shanyanshen electromagneticallydrivenintegratedmicrofluidicplatformusingreversetranscriptionloopmediatedisothermalamplificationfordetectionofsevereacuterespiratorysyndromecoronavirus2
AT leegwobin electromagneticallydrivenintegratedmicrofluidicplatformusingreversetranscriptionloopmediatedisothermalamplificationfordetectionofsevereacuterespiratorysyndromecoronavirus2