Quantification of ruffle area and dynamics in live or fixed lung adenocarcinoma cells

Ruffles are actin-rich membrane protrusions implicated in actin reorganization and initiation of cell motility. Here, we describe methods for measuring and analyzing ruffle dynamics in live cells and average ruffle area per cell in fixed samples. The specific steps described are for the analysis of...

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Detalles Bibliográficos
Autores principales: Kreider-Letterman, Gabriel, Cooke, Mariana, Goicoechea, Silvia M., Kazanietz, Marcelo G., Garcia-Mata, Rafael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9168141/
https://www.ncbi.nlm.nih.gov/pubmed/35677607
http://dx.doi.org/10.1016/j.xpro.2022.101437
Descripción
Sumario:Ruffles are actin-rich membrane protrusions implicated in actin reorganization and initiation of cell motility. Here, we describe methods for measuring and analyzing ruffle dynamics in live cells and average ruffle area per cell in fixed samples. The specific steps described are for the analysis of A549 lung adenocarcinoma cells, but the protocol can be applied to other cell types. The protocol has applications for dissecting the signaling events linked to ruffling. For complete details on the use and execution of this protocol, please refer to Cooke et al. (2021).

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