Quantification of ruffle area and dynamics in live or fixed lung adenocarcinoma cells

Ruffles are actin-rich membrane protrusions implicated in actin reorganization and initiation of cell motility. Here, we describe methods for measuring and analyzing ruffle dynamics in live cells and average ruffle area per cell in fixed samples. The specific steps described are for the analysis of...

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Autores principales: Kreider-Letterman, Gabriel, Cooke, Mariana, Goicoechea, Silvia M., Kazanietz, Marcelo G., Garcia-Mata, Rafael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9168141/
https://www.ncbi.nlm.nih.gov/pubmed/35677607
http://dx.doi.org/10.1016/j.xpro.2022.101437
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author Kreider-Letterman, Gabriel
Cooke, Mariana
Goicoechea, Silvia M.
Kazanietz, Marcelo G.
Garcia-Mata, Rafael
author_facet Kreider-Letterman, Gabriel
Cooke, Mariana
Goicoechea, Silvia M.
Kazanietz, Marcelo G.
Garcia-Mata, Rafael
author_sort Kreider-Letterman, Gabriel
collection PubMed
description Ruffles are actin-rich membrane protrusions implicated in actin reorganization and initiation of cell motility. Here, we describe methods for measuring and analyzing ruffle dynamics in live cells and average ruffle area per cell in fixed samples. The specific steps described are for the analysis of A549 lung adenocarcinoma cells, but the protocol can be applied to other cell types. The protocol has applications for dissecting the signaling events linked to ruffling. For complete details on the use and execution of this protocol, please refer to Cooke et al. (2021).
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spelling pubmed-91681412022-06-07 Quantification of ruffle area and dynamics in live or fixed lung adenocarcinoma cells Kreider-Letterman, Gabriel Cooke, Mariana Goicoechea, Silvia M. Kazanietz, Marcelo G. Garcia-Mata, Rafael STAR Protoc Protocol Ruffles are actin-rich membrane protrusions implicated in actin reorganization and initiation of cell motility. Here, we describe methods for measuring and analyzing ruffle dynamics in live cells and average ruffle area per cell in fixed samples. The specific steps described are for the analysis of A549 lung adenocarcinoma cells, but the protocol can be applied to other cell types. The protocol has applications for dissecting the signaling events linked to ruffling. For complete details on the use and execution of this protocol, please refer to Cooke et al. (2021). Elsevier 2022-06-04 /pmc/articles/PMC9168141/ /pubmed/35677607 http://dx.doi.org/10.1016/j.xpro.2022.101437 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Kreider-Letterman, Gabriel
Cooke, Mariana
Goicoechea, Silvia M.
Kazanietz, Marcelo G.
Garcia-Mata, Rafael
Quantification of ruffle area and dynamics in live or fixed lung adenocarcinoma cells
title Quantification of ruffle area and dynamics in live or fixed lung adenocarcinoma cells
title_full Quantification of ruffle area and dynamics in live or fixed lung adenocarcinoma cells
title_fullStr Quantification of ruffle area and dynamics in live or fixed lung adenocarcinoma cells
title_full_unstemmed Quantification of ruffle area and dynamics in live or fixed lung adenocarcinoma cells
title_short Quantification of ruffle area and dynamics in live or fixed lung adenocarcinoma cells
title_sort quantification of ruffle area and dynamics in live or fixed lung adenocarcinoma cells
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9168141/
https://www.ncbi.nlm.nih.gov/pubmed/35677607
http://dx.doi.org/10.1016/j.xpro.2022.101437
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