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Quantification of ruffle area and dynamics in live or fixed lung adenocarcinoma cells
Ruffles are actin-rich membrane protrusions implicated in actin reorganization and initiation of cell motility. Here, we describe methods for measuring and analyzing ruffle dynamics in live cells and average ruffle area per cell in fixed samples. The specific steps described are for the analysis of...
Autores principales: | Kreider-Letterman, Gabriel, Cooke, Mariana, Goicoechea, Silvia M., Kazanietz, Marcelo G., Garcia-Mata, Rafael |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9168141/ https://www.ncbi.nlm.nih.gov/pubmed/35677607 http://dx.doi.org/10.1016/j.xpro.2022.101437 |
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