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Evaluating the glycolytic potential of mouse costimulated effector CD8(+) T cells ex vivo

Studying the metabolic fitness of T cells is fundamental to understand how immune responses are regulated. Here, we describe a step-by-step protocol optimized to efficiently generate and isolate effector antigen-specific CD8(+) T cells ex vivo using costimulation. We also detail steps to evaluate th...

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Detalles Bibliográficos
Autores principales: Agliano, Federica, Ménoret, Antoine, Vella, Anthony T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9168147/
https://www.ncbi.nlm.nih.gov/pubmed/35677608
http://dx.doi.org/10.1016/j.xpro.2022.101441