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Evaluating the glycolytic potential of mouse costimulated effector CD8(+) T cells ex vivo
Studying the metabolic fitness of T cells is fundamental to understand how immune responses are regulated. Here, we describe a step-by-step protocol optimized to efficiently generate and isolate effector antigen-specific CD8(+) T cells ex vivo using costimulation. We also detail steps to evaluate th...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9168147/ https://www.ncbi.nlm.nih.gov/pubmed/35677608 http://dx.doi.org/10.1016/j.xpro.2022.101441 |