Optical analysis of AMPAR-mediated synaptic scaling in mouse hippocampus

Immunolabeling of surface AMPA receptors (AMPARs) can be used for in vivo or ex vivo examination of synaptic scaling, a type of homeostatic plasticity. Here, we present a protocol to analyze changes in synaptic weights using immunohistochemistry for surface AMPARs coupled with optical imaging analys...

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Detalles Bibliográficos
Autores principales: Suzuki, Kanzo, Kavalali, Ege T., Monteggia, Lisa M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9168154/
https://www.ncbi.nlm.nih.gov/pubmed/35677613
http://dx.doi.org/10.1016/j.xpro.2022.101443
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author Suzuki, Kanzo
Kavalali, Ege T.
Monteggia, Lisa M.
author_facet Suzuki, Kanzo
Kavalali, Ege T.
Monteggia, Lisa M.
author_sort Suzuki, Kanzo
collection PubMed
description Immunolabeling of surface AMPA receptors (AMPARs) can be used for in vivo or ex vivo examination of synaptic scaling, a type of homeostatic plasticity. Here, we present a protocol to analyze changes in synaptic weights using immunohistochemistry for surface AMPARs coupled with optical imaging analysis. We detail immunostaining of AMPARs in mouse brain sections, followed by confocal imaging of surface AMPARs in dendritic region of hippocampal CA1. We then describe using Fiji/ImageJ and rank order plots for analyzing synaptic weight. For complete details on the use and execution of this protocol, please refer to Suzuki et al. (2021).
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spelling pubmed-91681542022-06-07 Optical analysis of AMPAR-mediated synaptic scaling in mouse hippocampus Suzuki, Kanzo Kavalali, Ege T. Monteggia, Lisa M. STAR Protoc Protocol Immunolabeling of surface AMPA receptors (AMPARs) can be used for in vivo or ex vivo examination of synaptic scaling, a type of homeostatic plasticity. Here, we present a protocol to analyze changes in synaptic weights using immunohistochemistry for surface AMPARs coupled with optical imaging analysis. We detail immunostaining of AMPARs in mouse brain sections, followed by confocal imaging of surface AMPARs in dendritic region of hippocampal CA1. We then describe using Fiji/ImageJ and rank order plots for analyzing synaptic weight. For complete details on the use and execution of this protocol, please refer to Suzuki et al. (2021). Elsevier 2022-06-04 /pmc/articles/PMC9168154/ /pubmed/35677613 http://dx.doi.org/10.1016/j.xpro.2022.101443 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Suzuki, Kanzo
Kavalali, Ege T.
Monteggia, Lisa M.
Optical analysis of AMPAR-mediated synaptic scaling in mouse hippocampus
title Optical analysis of AMPAR-mediated synaptic scaling in mouse hippocampus
title_full Optical analysis of AMPAR-mediated synaptic scaling in mouse hippocampus
title_fullStr Optical analysis of AMPAR-mediated synaptic scaling in mouse hippocampus
title_full_unstemmed Optical analysis of AMPAR-mediated synaptic scaling in mouse hippocampus
title_short Optical analysis of AMPAR-mediated synaptic scaling in mouse hippocampus
title_sort optical analysis of ampar-mediated synaptic scaling in mouse hippocampus
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9168154/
https://www.ncbi.nlm.nih.gov/pubmed/35677613
http://dx.doi.org/10.1016/j.xpro.2022.101443
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