Flow cytometry analysis of endothelial cells and subsets of exhausted CD8+ T cells in murine tumor models

Here, we present a protocol for flow cytometry analysis of endothelial cells (ECs) and CD8+ T cells in murine tumor models, at baseline and after cancer immunotherapy with anti-PD-1/anti-CTLA-4 antibodies. We provide gating strategies for identification of specific cell subsets including ECs from tu...

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Detalles Bibliográficos
Autores principales: Blanchard, Lucas, Vina, Estefania, Asrir, Assia, Tardiveau, Claire, Coudert, Juliette, Laffont, Robin, Tarroux, Dorian, Bettini, Sarah, Veerman, Krystle, Lafouresse, Fanny, Pichery, Mélanie, Mirey, Emilie, Bellard, Elisabeth, Ortega, Nathalie, Girard, Jean-Philippe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9168162/
https://www.ncbi.nlm.nih.gov/pubmed/35677615
http://dx.doi.org/10.1016/j.xpro.2022.101444

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