Protocol for isolating adult pituitary stem/progenitor cells in mice

Hormone-producing cells in the anterior lobe (AL) of the pituitary gland are important for growth and reproduction, but it has been challenging to isolate their source: the pituitary stem/progenitor cells. Here, we present a protocol for isolating adult pituitary stem/progenitor cells (APSCs) in mic...

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Detalles Bibliográficos
Autores principales: Shintani, Aran, Higuchi, Masashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9168163/
https://www.ncbi.nlm.nih.gov/pubmed/35677611
http://dx.doi.org/10.1016/j.xpro.2022.101420
Descripción
Sumario:Hormone-producing cells in the anterior lobe (AL) of the pituitary gland are important for growth and reproduction, but it has been challenging to isolate their source: the pituitary stem/progenitor cells. Here, we present a protocol for isolating adult pituitary stem/progenitor cells (APSCs) in mice. We describe dissociation and culture of AL cells, followed by the assessments of stemness marker expression and the differentiation capacity. This protocol enables separation of APSCs based on their cell adhesion properties with nearly 100% purity. For complete details on the use and execution of this protocol, please refer to Shintani and Higuchi (2021).

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