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Late-Onset Calorie Restriction Improves Lipid Metabolism and Aggravates Inflammation in the Liver of Old Wistar Rats

Aging is a progressive process that could disturb metabolic homeostasis in the liver via ectopic lipid accumulation, oxidative stress, and deterioration of inflammatory response. Although calorie restriction (CR) is recognized as beneficial for life span and health span prolongation, it is still unc...

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Autores principales: Teofilović, Ana, Vratarić, Miloš, Veličković, Nataša, Vojnović Milutinović, Danijela, Mladenovic, Aleksandra, Prvulovic, Milica, Djordjevic, Ana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9168317/
https://www.ncbi.nlm.nih.gov/pubmed/35677539
http://dx.doi.org/10.3389/fnut.2022.899255
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author Teofilović, Ana
Vratarić, Miloš
Veličković, Nataša
Vojnović Milutinović, Danijela
Mladenovic, Aleksandra
Prvulovic, Milica
Djordjevic, Ana
author_facet Teofilović, Ana
Vratarić, Miloš
Veličković, Nataša
Vojnović Milutinović, Danijela
Mladenovic, Aleksandra
Prvulovic, Milica
Djordjevic, Ana
author_sort Teofilović, Ana
collection PubMed
description Aging is a progressive process that could disturb metabolic homeostasis in the liver via ectopic lipid accumulation, oxidative stress, and deterioration of inflammatory response. Although calorie restriction (CR) is recognized as beneficial for life span and health span prolongation, it is still unclear how late-onset CR, characterized by late beginning and short duration, affects age-related processes. The aim of this study was to examine how late-onset CR-induced metabolic adjustments impact lipid status and inflammation in the liver of old rats. The experiments were conducted on aging male Wistar rats fed ad libitum (AL) or exposed to late-onset CR (60% of AL daily intake) from 21st to 24th month. The results showed that late-onset CR reduces body weight, visceral adipose tissue and liver mass, and triglyceride levels when compared to old animals on AL diet. The ameliorating effects of CR on lipid metabolism include increased activity of AMP-activated protein kinase, suppressed de novo fatty acid synthesis, stimulated β-oxidation, decreased lipotoxicity, and limited triglyceride synthesis and packaging in the liver. Restricted diet regime, however, does not improve expression of antioxidant enzymes, although it leads to progression of age-related inflammation in the liver, partially through lower corticosterone concentration and decreased activation of glucocorticoid receptor. In conclusion, late-onset CR is able to restore age-related imbalance of lipid metabolism in the liver, but has a negative impact on hepatic inflammatory status, implying that the type of diet for older individuals must be balanced and chosen carefully with appropriate duration and start point.
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spelling pubmed-91683172022-06-07 Late-Onset Calorie Restriction Improves Lipid Metabolism and Aggravates Inflammation in the Liver of Old Wistar Rats Teofilović, Ana Vratarić, Miloš Veličković, Nataša Vojnović Milutinović, Danijela Mladenovic, Aleksandra Prvulovic, Milica Djordjevic, Ana Front Nutr Nutrition Aging is a progressive process that could disturb metabolic homeostasis in the liver via ectopic lipid accumulation, oxidative stress, and deterioration of inflammatory response. Although calorie restriction (CR) is recognized as beneficial for life span and health span prolongation, it is still unclear how late-onset CR, characterized by late beginning and short duration, affects age-related processes. The aim of this study was to examine how late-onset CR-induced metabolic adjustments impact lipid status and inflammation in the liver of old rats. The experiments were conducted on aging male Wistar rats fed ad libitum (AL) or exposed to late-onset CR (60% of AL daily intake) from 21st to 24th month. The results showed that late-onset CR reduces body weight, visceral adipose tissue and liver mass, and triglyceride levels when compared to old animals on AL diet. The ameliorating effects of CR on lipid metabolism include increased activity of AMP-activated protein kinase, suppressed de novo fatty acid synthesis, stimulated β-oxidation, decreased lipotoxicity, and limited triglyceride synthesis and packaging in the liver. Restricted diet regime, however, does not improve expression of antioxidant enzymes, although it leads to progression of age-related inflammation in the liver, partially through lower corticosterone concentration and decreased activation of glucocorticoid receptor. In conclusion, late-onset CR is able to restore age-related imbalance of lipid metabolism in the liver, but has a negative impact on hepatic inflammatory status, implying that the type of diet for older individuals must be balanced and chosen carefully with appropriate duration and start point. Frontiers Media S.A. 2022-05-23 /pmc/articles/PMC9168317/ /pubmed/35677539 http://dx.doi.org/10.3389/fnut.2022.899255 Text en Copyright © 2022 Teofilović, Vratarić, Veličković, Vojnović Milutinović, Mladenovic, Prvulovic and Djordjevic. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Nutrition
Teofilović, Ana
Vratarić, Miloš
Veličković, Nataša
Vojnović Milutinović, Danijela
Mladenovic, Aleksandra
Prvulovic, Milica
Djordjevic, Ana
Late-Onset Calorie Restriction Improves Lipid Metabolism and Aggravates Inflammation in the Liver of Old Wistar Rats
title Late-Onset Calorie Restriction Improves Lipid Metabolism and Aggravates Inflammation in the Liver of Old Wistar Rats
title_full Late-Onset Calorie Restriction Improves Lipid Metabolism and Aggravates Inflammation in the Liver of Old Wistar Rats
title_fullStr Late-Onset Calorie Restriction Improves Lipid Metabolism and Aggravates Inflammation in the Liver of Old Wistar Rats
title_full_unstemmed Late-Onset Calorie Restriction Improves Lipid Metabolism and Aggravates Inflammation in the Liver of Old Wistar Rats
title_short Late-Onset Calorie Restriction Improves Lipid Metabolism and Aggravates Inflammation in the Liver of Old Wistar Rats
title_sort late-onset calorie restriction improves lipid metabolism and aggravates inflammation in the liver of old wistar rats
topic Nutrition
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9168317/
https://www.ncbi.nlm.nih.gov/pubmed/35677539
http://dx.doi.org/10.3389/fnut.2022.899255
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