Cargando…
Proteomic Analysis Reveals Proteins Involved in the Mode of Action of β-Citronellol Identified From Citrus hystrix DC. Leaf Against Candida albicans
Candida albicans is a fungus that lives primarily on the mucosal surfaces of healthy humans, such as the oral cavity, vagina, and gastrointestinal tract. This commensal organism can be controlled by other microbiota, while certain conditions can increase the risk of C. albicans outgrowth and cause d...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9168680/ https://www.ncbi.nlm.nih.gov/pubmed/35677908 http://dx.doi.org/10.3389/fmicb.2022.894637 |
_version_ | 1784721057521860608 |
---|---|
author | Buakaew, Watunyoo Pankla Sranujit, Rungnapa Noysang, Chanai Krobthong, Sucheewin Yingchutrakul, Yodying Thongsri, Yordhathai Potup, Pachuen Daowtak, Krai Usuwanthim, Kanchana |
author_facet | Buakaew, Watunyoo Pankla Sranujit, Rungnapa Noysang, Chanai Krobthong, Sucheewin Yingchutrakul, Yodying Thongsri, Yordhathai Potup, Pachuen Daowtak, Krai Usuwanthim, Kanchana |
author_sort | Buakaew, Watunyoo |
collection | PubMed |
description | Candida albicans is a fungus that lives primarily on the mucosal surfaces of healthy humans, such as the oral cavity, vagina, and gastrointestinal tract. This commensal organism can be controlled by other microbiota, while certain conditions can increase the risk of C. albicans outgrowth and cause disease. Prevalence of the drug-resistant phenotype, as well as the severity of C. albicans infection in immunocompromised patients, presents a challenge for scientists to develop novel, effective treatment, and prevention strategies. β-Citronellol is an intriguing active compound of several plants that has been linked to antifungal activity, but data on the mechanism of action in terms of proteomic profiling are lacking. Here, β-citronellol identified from Citrus hystrix DC. leaf against C. albicans were evaluated. A proteomic approach was used to identify potential target proteins involved in the mode of action of β-citronellol. This study identified and discussed three protein groups based on the 126 major proteins that were altered in response to β-citronellol treatment, 46 of which were downregulated and 80 of which were upregulated. Significant protein groups include cell wall proteins (e.g., Als2p, Rbt1p, and Pga4p), cellular stress response enzymes (e.g., Sod1p, Gst2p, and Ddr48p), and ATP synthesis-associated proteins (e.g., Atp3p, Atp7p, Cox1p, and Cobp). Results demonstrated the complexities of protein interactions influenced by β-citronellol treatment and highlighted the potential of antifungal activity for future clinical and drug development research. |
format | Online Article Text |
id | pubmed-9168680 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-91686802022-06-07 Proteomic Analysis Reveals Proteins Involved in the Mode of Action of β-Citronellol Identified From Citrus hystrix DC. Leaf Against Candida albicans Buakaew, Watunyoo Pankla Sranujit, Rungnapa Noysang, Chanai Krobthong, Sucheewin Yingchutrakul, Yodying Thongsri, Yordhathai Potup, Pachuen Daowtak, Krai Usuwanthim, Kanchana Front Microbiol Microbiology Candida albicans is a fungus that lives primarily on the mucosal surfaces of healthy humans, such as the oral cavity, vagina, and gastrointestinal tract. This commensal organism can be controlled by other microbiota, while certain conditions can increase the risk of C. albicans outgrowth and cause disease. Prevalence of the drug-resistant phenotype, as well as the severity of C. albicans infection in immunocompromised patients, presents a challenge for scientists to develop novel, effective treatment, and prevention strategies. β-Citronellol is an intriguing active compound of several plants that has been linked to antifungal activity, but data on the mechanism of action in terms of proteomic profiling are lacking. Here, β-citronellol identified from Citrus hystrix DC. leaf against C. albicans were evaluated. A proteomic approach was used to identify potential target proteins involved in the mode of action of β-citronellol. This study identified and discussed three protein groups based on the 126 major proteins that were altered in response to β-citronellol treatment, 46 of which were downregulated and 80 of which were upregulated. Significant protein groups include cell wall proteins (e.g., Als2p, Rbt1p, and Pga4p), cellular stress response enzymes (e.g., Sod1p, Gst2p, and Ddr48p), and ATP synthesis-associated proteins (e.g., Atp3p, Atp7p, Cox1p, and Cobp). Results demonstrated the complexities of protein interactions influenced by β-citronellol treatment and highlighted the potential of antifungal activity for future clinical and drug development research. Frontiers Media S.A. 2022-05-23 /pmc/articles/PMC9168680/ /pubmed/35677908 http://dx.doi.org/10.3389/fmicb.2022.894637 Text en Copyright © 2022 Buakaew, Pankla Sranujit, Noysang, Krobthong, Yingchutrakul, Thongsri, Potup, Daowtak and Usuwanthim. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Buakaew, Watunyoo Pankla Sranujit, Rungnapa Noysang, Chanai Krobthong, Sucheewin Yingchutrakul, Yodying Thongsri, Yordhathai Potup, Pachuen Daowtak, Krai Usuwanthim, Kanchana Proteomic Analysis Reveals Proteins Involved in the Mode of Action of β-Citronellol Identified From Citrus hystrix DC. Leaf Against Candida albicans |
title | Proteomic Analysis Reveals Proteins Involved in the Mode of Action of β-Citronellol Identified From Citrus hystrix DC. Leaf Against Candida albicans |
title_full | Proteomic Analysis Reveals Proteins Involved in the Mode of Action of β-Citronellol Identified From Citrus hystrix DC. Leaf Against Candida albicans |
title_fullStr | Proteomic Analysis Reveals Proteins Involved in the Mode of Action of β-Citronellol Identified From Citrus hystrix DC. Leaf Against Candida albicans |
title_full_unstemmed | Proteomic Analysis Reveals Proteins Involved in the Mode of Action of β-Citronellol Identified From Citrus hystrix DC. Leaf Against Candida albicans |
title_short | Proteomic Analysis Reveals Proteins Involved in the Mode of Action of β-Citronellol Identified From Citrus hystrix DC. Leaf Against Candida albicans |
title_sort | proteomic analysis reveals proteins involved in the mode of action of β-citronellol identified from citrus hystrix dc. leaf against candida albicans |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9168680/ https://www.ncbi.nlm.nih.gov/pubmed/35677908 http://dx.doi.org/10.3389/fmicb.2022.894637 |
work_keys_str_mv | AT buakaewwatunyoo proteomicanalysisrevealsproteinsinvolvedinthemodeofactionofbcitronellolidentifiedfromcitrushystrixdcleafagainstcandidaalbicans AT panklasranujitrungnapa proteomicanalysisrevealsproteinsinvolvedinthemodeofactionofbcitronellolidentifiedfromcitrushystrixdcleafagainstcandidaalbicans AT noysangchanai proteomicanalysisrevealsproteinsinvolvedinthemodeofactionofbcitronellolidentifiedfromcitrushystrixdcleafagainstcandidaalbicans AT krobthongsucheewin proteomicanalysisrevealsproteinsinvolvedinthemodeofactionofbcitronellolidentifiedfromcitrushystrixdcleafagainstcandidaalbicans AT yingchutrakulyodying proteomicanalysisrevealsproteinsinvolvedinthemodeofactionofbcitronellolidentifiedfromcitrushystrixdcleafagainstcandidaalbicans AT thongsriyordhathai proteomicanalysisrevealsproteinsinvolvedinthemodeofactionofbcitronellolidentifiedfromcitrushystrixdcleafagainstcandidaalbicans AT potuppachuen proteomicanalysisrevealsproteinsinvolvedinthemodeofactionofbcitronellolidentifiedfromcitrushystrixdcleafagainstcandidaalbicans AT daowtakkrai proteomicanalysisrevealsproteinsinvolvedinthemodeofactionofbcitronellolidentifiedfromcitrushystrixdcleafagainstcandidaalbicans AT usuwanthimkanchana proteomicanalysisrevealsproteinsinvolvedinthemodeofactionofbcitronellolidentifiedfromcitrushystrixdcleafagainstcandidaalbicans |