Cargando…

Paired Donor and Recipient Immunophenotyping in Allogeneic Hematopoietic Stem Cell Transplantation: A Cellular Network Approach

Success and complications of allogeneic hematopoietic stem cell transplantation (alloHSCT) are closely connected to the transferred graft and immune reconstitution post alloHSCT. Due to the variety of immune cells and their distinct roles, a broad evaluation of the immune cellular network is warrant...

Descripción completa

Detalles Bibliográficos
Autores principales: Wittenbecher, Friedrich, Lesch, Stella, Kolling, Stefan, Blau, Igor-Wolfgang, Vuong, Lam, Borchert, Franziska, Movasshagi, Kamran, Tietze-Bürger, Carola, Penack, Olaf, Ahn, Johann, Bullinger, Lars, Frentsch, Marco, Na, Il-Kang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9168993/
https://www.ncbi.nlm.nih.gov/pubmed/35677053
http://dx.doi.org/10.3389/fimmu.2022.874499
_version_ 1784721117168009216
author Wittenbecher, Friedrich
Lesch, Stella
Kolling, Stefan
Blau, Igor-Wolfgang
Vuong, Lam
Borchert, Franziska
Movasshagi, Kamran
Tietze-Bürger, Carola
Penack, Olaf
Ahn, Johann
Bullinger, Lars
Frentsch, Marco
Na, Il-Kang
author_facet Wittenbecher, Friedrich
Lesch, Stella
Kolling, Stefan
Blau, Igor-Wolfgang
Vuong, Lam
Borchert, Franziska
Movasshagi, Kamran
Tietze-Bürger, Carola
Penack, Olaf
Ahn, Johann
Bullinger, Lars
Frentsch, Marco
Na, Il-Kang
author_sort Wittenbecher, Friedrich
collection PubMed
description Success and complications of allogeneic hematopoietic stem cell transplantation (alloHSCT) are closely connected to the transferred graft and immune reconstitution post alloHSCT. Due to the variety of immune cells and their distinct roles, a broad evaluation of the immune cellular network is warranted in mobilization and reconstitution studies in alloHSCT. Here, we propose a comprehensive phenotypic analysis of 26 immune cell subsets with multicolor flow cytometry from only 100µl whole blood per time point. Using this approach, we provide an extensive longitudinal analysis of almost 200 time points from 21 donor-recipient pairs. We observe a broad mobilization of innate and adaptive immune cell subsets after granulocyte-colony stimulating factor (G-CSF) treatment of healthy donors. Our data suggest that the relative quantitative immune cell subset composition in recipients approaches that of healthy donors from day +180 post alloHSCT onwards. Correlation of donor and recipient cell counts reveals distinct association patterns for different immune cell subsets and hierarchical clustering of recipient cell counts identifies distinct reconstitution groups in the first month after transplantation. We suggest our comprehensive immune subset analysis as a feasible and time efficient approach for a broad immune assessment for future clinical studies in the context of alloHSCT. This comprehensive cell composition assessment can be a critical step towards personalized graft composition strategies and individualized therapy management in areas such as GvHD prophylaxis in the highly complex immunological setting of alloHSCT.
format Online
Article
Text
id pubmed-9168993
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-91689932022-06-07 Paired Donor and Recipient Immunophenotyping in Allogeneic Hematopoietic Stem Cell Transplantation: A Cellular Network Approach Wittenbecher, Friedrich Lesch, Stella Kolling, Stefan Blau, Igor-Wolfgang Vuong, Lam Borchert, Franziska Movasshagi, Kamran Tietze-Bürger, Carola Penack, Olaf Ahn, Johann Bullinger, Lars Frentsch, Marco Na, Il-Kang Front Immunol Immunology Success and complications of allogeneic hematopoietic stem cell transplantation (alloHSCT) are closely connected to the transferred graft and immune reconstitution post alloHSCT. Due to the variety of immune cells and their distinct roles, a broad evaluation of the immune cellular network is warranted in mobilization and reconstitution studies in alloHSCT. Here, we propose a comprehensive phenotypic analysis of 26 immune cell subsets with multicolor flow cytometry from only 100µl whole blood per time point. Using this approach, we provide an extensive longitudinal analysis of almost 200 time points from 21 donor-recipient pairs. We observe a broad mobilization of innate and adaptive immune cell subsets after granulocyte-colony stimulating factor (G-CSF) treatment of healthy donors. Our data suggest that the relative quantitative immune cell subset composition in recipients approaches that of healthy donors from day +180 post alloHSCT onwards. Correlation of donor and recipient cell counts reveals distinct association patterns for different immune cell subsets and hierarchical clustering of recipient cell counts identifies distinct reconstitution groups in the first month after transplantation. We suggest our comprehensive immune subset analysis as a feasible and time efficient approach for a broad immune assessment for future clinical studies in the context of alloHSCT. This comprehensive cell composition assessment can be a critical step towards personalized graft composition strategies and individualized therapy management in areas such as GvHD prophylaxis in the highly complex immunological setting of alloHSCT. Frontiers Media S.A. 2022-05-23 /pmc/articles/PMC9168993/ /pubmed/35677053 http://dx.doi.org/10.3389/fimmu.2022.874499 Text en Copyright © 2022 Wittenbecher, Lesch, Kolling, Blau, Vuong, Borchert, Movasshagi, Tietze-Bürger, Penack, Ahn, Bullinger, Frentsch and Na https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Wittenbecher, Friedrich
Lesch, Stella
Kolling, Stefan
Blau, Igor-Wolfgang
Vuong, Lam
Borchert, Franziska
Movasshagi, Kamran
Tietze-Bürger, Carola
Penack, Olaf
Ahn, Johann
Bullinger, Lars
Frentsch, Marco
Na, Il-Kang
Paired Donor and Recipient Immunophenotyping in Allogeneic Hematopoietic Stem Cell Transplantation: A Cellular Network Approach
title Paired Donor and Recipient Immunophenotyping in Allogeneic Hematopoietic Stem Cell Transplantation: A Cellular Network Approach
title_full Paired Donor and Recipient Immunophenotyping in Allogeneic Hematopoietic Stem Cell Transplantation: A Cellular Network Approach
title_fullStr Paired Donor and Recipient Immunophenotyping in Allogeneic Hematopoietic Stem Cell Transplantation: A Cellular Network Approach
title_full_unstemmed Paired Donor and Recipient Immunophenotyping in Allogeneic Hematopoietic Stem Cell Transplantation: A Cellular Network Approach
title_short Paired Donor and Recipient Immunophenotyping in Allogeneic Hematopoietic Stem Cell Transplantation: A Cellular Network Approach
title_sort paired donor and recipient immunophenotyping in allogeneic hematopoietic stem cell transplantation: a cellular network approach
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9168993/
https://www.ncbi.nlm.nih.gov/pubmed/35677053
http://dx.doi.org/10.3389/fimmu.2022.874499
work_keys_str_mv AT wittenbecherfriedrich paireddonorandrecipientimmunophenotypinginallogeneichematopoieticstemcelltransplantationacellularnetworkapproach
AT leschstella paireddonorandrecipientimmunophenotypinginallogeneichematopoieticstemcelltransplantationacellularnetworkapproach
AT kollingstefan paireddonorandrecipientimmunophenotypinginallogeneichematopoieticstemcelltransplantationacellularnetworkapproach
AT blauigorwolfgang paireddonorandrecipientimmunophenotypinginallogeneichematopoieticstemcelltransplantationacellularnetworkapproach
AT vuonglam paireddonorandrecipientimmunophenotypinginallogeneichematopoieticstemcelltransplantationacellularnetworkapproach
AT borchertfranziska paireddonorandrecipientimmunophenotypinginallogeneichematopoieticstemcelltransplantationacellularnetworkapproach
AT movasshagikamran paireddonorandrecipientimmunophenotypinginallogeneichematopoieticstemcelltransplantationacellularnetworkapproach
AT tietzeburgercarola paireddonorandrecipientimmunophenotypinginallogeneichematopoieticstemcelltransplantationacellularnetworkapproach
AT penackolaf paireddonorandrecipientimmunophenotypinginallogeneichematopoieticstemcelltransplantationacellularnetworkapproach
AT ahnjohann paireddonorandrecipientimmunophenotypinginallogeneichematopoieticstemcelltransplantationacellularnetworkapproach
AT bullingerlars paireddonorandrecipientimmunophenotypinginallogeneichematopoieticstemcelltransplantationacellularnetworkapproach
AT frentschmarco paireddonorandrecipientimmunophenotypinginallogeneichematopoieticstemcelltransplantationacellularnetworkapproach
AT nailkang paireddonorandrecipientimmunophenotypinginallogeneichematopoieticstemcelltransplantationacellularnetworkapproach