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Paired Donor and Recipient Immunophenotyping in Allogeneic Hematopoietic Stem Cell Transplantation: A Cellular Network Approach
Success and complications of allogeneic hematopoietic stem cell transplantation (alloHSCT) are closely connected to the transferred graft and immune reconstitution post alloHSCT. Due to the variety of immune cells and their distinct roles, a broad evaluation of the immune cellular network is warrant...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9168993/ https://www.ncbi.nlm.nih.gov/pubmed/35677053 http://dx.doi.org/10.3389/fimmu.2022.874499 |
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author | Wittenbecher, Friedrich Lesch, Stella Kolling, Stefan Blau, Igor-Wolfgang Vuong, Lam Borchert, Franziska Movasshagi, Kamran Tietze-Bürger, Carola Penack, Olaf Ahn, Johann Bullinger, Lars Frentsch, Marco Na, Il-Kang |
author_facet | Wittenbecher, Friedrich Lesch, Stella Kolling, Stefan Blau, Igor-Wolfgang Vuong, Lam Borchert, Franziska Movasshagi, Kamran Tietze-Bürger, Carola Penack, Olaf Ahn, Johann Bullinger, Lars Frentsch, Marco Na, Il-Kang |
author_sort | Wittenbecher, Friedrich |
collection | PubMed |
description | Success and complications of allogeneic hematopoietic stem cell transplantation (alloHSCT) are closely connected to the transferred graft and immune reconstitution post alloHSCT. Due to the variety of immune cells and their distinct roles, a broad evaluation of the immune cellular network is warranted in mobilization and reconstitution studies in alloHSCT. Here, we propose a comprehensive phenotypic analysis of 26 immune cell subsets with multicolor flow cytometry from only 100µl whole blood per time point. Using this approach, we provide an extensive longitudinal analysis of almost 200 time points from 21 donor-recipient pairs. We observe a broad mobilization of innate and adaptive immune cell subsets after granulocyte-colony stimulating factor (G-CSF) treatment of healthy donors. Our data suggest that the relative quantitative immune cell subset composition in recipients approaches that of healthy donors from day +180 post alloHSCT onwards. Correlation of donor and recipient cell counts reveals distinct association patterns for different immune cell subsets and hierarchical clustering of recipient cell counts identifies distinct reconstitution groups in the first month after transplantation. We suggest our comprehensive immune subset analysis as a feasible and time efficient approach for a broad immune assessment for future clinical studies in the context of alloHSCT. This comprehensive cell composition assessment can be a critical step towards personalized graft composition strategies and individualized therapy management in areas such as GvHD prophylaxis in the highly complex immunological setting of alloHSCT. |
format | Online Article Text |
id | pubmed-9168993 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-91689932022-06-07 Paired Donor and Recipient Immunophenotyping in Allogeneic Hematopoietic Stem Cell Transplantation: A Cellular Network Approach Wittenbecher, Friedrich Lesch, Stella Kolling, Stefan Blau, Igor-Wolfgang Vuong, Lam Borchert, Franziska Movasshagi, Kamran Tietze-Bürger, Carola Penack, Olaf Ahn, Johann Bullinger, Lars Frentsch, Marco Na, Il-Kang Front Immunol Immunology Success and complications of allogeneic hematopoietic stem cell transplantation (alloHSCT) are closely connected to the transferred graft and immune reconstitution post alloHSCT. Due to the variety of immune cells and their distinct roles, a broad evaluation of the immune cellular network is warranted in mobilization and reconstitution studies in alloHSCT. Here, we propose a comprehensive phenotypic analysis of 26 immune cell subsets with multicolor flow cytometry from only 100µl whole blood per time point. Using this approach, we provide an extensive longitudinal analysis of almost 200 time points from 21 donor-recipient pairs. We observe a broad mobilization of innate and adaptive immune cell subsets after granulocyte-colony stimulating factor (G-CSF) treatment of healthy donors. Our data suggest that the relative quantitative immune cell subset composition in recipients approaches that of healthy donors from day +180 post alloHSCT onwards. Correlation of donor and recipient cell counts reveals distinct association patterns for different immune cell subsets and hierarchical clustering of recipient cell counts identifies distinct reconstitution groups in the first month after transplantation. We suggest our comprehensive immune subset analysis as a feasible and time efficient approach for a broad immune assessment for future clinical studies in the context of alloHSCT. This comprehensive cell composition assessment can be a critical step towards personalized graft composition strategies and individualized therapy management in areas such as GvHD prophylaxis in the highly complex immunological setting of alloHSCT. Frontiers Media S.A. 2022-05-23 /pmc/articles/PMC9168993/ /pubmed/35677053 http://dx.doi.org/10.3389/fimmu.2022.874499 Text en Copyright © 2022 Wittenbecher, Lesch, Kolling, Blau, Vuong, Borchert, Movasshagi, Tietze-Bürger, Penack, Ahn, Bullinger, Frentsch and Na https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Wittenbecher, Friedrich Lesch, Stella Kolling, Stefan Blau, Igor-Wolfgang Vuong, Lam Borchert, Franziska Movasshagi, Kamran Tietze-Bürger, Carola Penack, Olaf Ahn, Johann Bullinger, Lars Frentsch, Marco Na, Il-Kang Paired Donor and Recipient Immunophenotyping in Allogeneic Hematopoietic Stem Cell Transplantation: A Cellular Network Approach |
title | Paired Donor and Recipient Immunophenotyping in Allogeneic Hematopoietic Stem Cell Transplantation: A Cellular Network Approach |
title_full | Paired Donor and Recipient Immunophenotyping in Allogeneic Hematopoietic Stem Cell Transplantation: A Cellular Network Approach |
title_fullStr | Paired Donor and Recipient Immunophenotyping in Allogeneic Hematopoietic Stem Cell Transplantation: A Cellular Network Approach |
title_full_unstemmed | Paired Donor and Recipient Immunophenotyping in Allogeneic Hematopoietic Stem Cell Transplantation: A Cellular Network Approach |
title_short | Paired Donor and Recipient Immunophenotyping in Allogeneic Hematopoietic Stem Cell Transplantation: A Cellular Network Approach |
title_sort | paired donor and recipient immunophenotyping in allogeneic hematopoietic stem cell transplantation: a cellular network approach |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9168993/ https://www.ncbi.nlm.nih.gov/pubmed/35677053 http://dx.doi.org/10.3389/fimmu.2022.874499 |
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