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Separation of Heterotrophic Microalgae Crypthecodinium cohnii by Dielectrophoresis
Microalgae constitute an abundant source of poly-unsaturated fatty acids which are applied in various biotechnological fields such as pharmaceuticals and food supplement. Separating microalgae cells with respect to their lipid content would establish a relevant at-line analytical technique. The pres...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9169251/ https://www.ncbi.nlm.nih.gov/pubmed/35677299 http://dx.doi.org/10.3389/fbioe.2022.855035 |
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author | Birkholz, Mario Malti, Danai Eleni Hartmann, Stephan Neubauer, Peter |
author_facet | Birkholz, Mario Malti, Danai Eleni Hartmann, Stephan Neubauer, Peter |
author_sort | Birkholz, Mario |
collection | PubMed |
description | Microalgae constitute an abundant source of poly-unsaturated fatty acids which are applied in various biotechnological fields such as pharmaceuticals and food supplement. Separating microalgae cells with respect to their lipid content would establish a relevant at-line analytical technique. The present study demonstrates an electrical approach for the separation of the lipid-producing microalgae Crypthecodinium cohnii using the effect of dielectrophoresis (DEP) in a microfluidic flow cell. Microalgae were cultivated for 8 days, while cell growth was characterized by optical density, dry cell weight, glucose concentration and lipid content via fluorescence microscopy. The size distribution of cells during cultivation was thoroughly investigated, since the DEP force scales with cell volume, but also depends on lipid content via cell electrophysiological constants. Thus, the challenge was to deconvolute one separation effect from the other, while the electrical cell constants of C. cohnii are not known yet. The DEP-dependent separation was realized by slanted top-bottom electrodes with the flowing cell suspension between them. Turning on the voltage deflected the cells from their initial path as determined by the streaming and thus changed their direction of flow. The separation efficiency of DEP was tested for various electrical field strengths and its performance was determined by quantitative analysis of optical and fluorescence videos. It could be shown for all size groups that the most lipid-containing cells were always subject to DEP separation and that the method is thus not only suitable for process analysis, but also for strain selection of the most productive cell lines. |
format | Online Article Text |
id | pubmed-9169251 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-91692512022-06-07 Separation of Heterotrophic Microalgae Crypthecodinium cohnii by Dielectrophoresis Birkholz, Mario Malti, Danai Eleni Hartmann, Stephan Neubauer, Peter Front Bioeng Biotechnol Bioengineering and Biotechnology Microalgae constitute an abundant source of poly-unsaturated fatty acids which are applied in various biotechnological fields such as pharmaceuticals and food supplement. Separating microalgae cells with respect to their lipid content would establish a relevant at-line analytical technique. The present study demonstrates an electrical approach for the separation of the lipid-producing microalgae Crypthecodinium cohnii using the effect of dielectrophoresis (DEP) in a microfluidic flow cell. Microalgae were cultivated for 8 days, while cell growth was characterized by optical density, dry cell weight, glucose concentration and lipid content via fluorescence microscopy. The size distribution of cells during cultivation was thoroughly investigated, since the DEP force scales with cell volume, but also depends on lipid content via cell electrophysiological constants. Thus, the challenge was to deconvolute one separation effect from the other, while the electrical cell constants of C. cohnii are not known yet. The DEP-dependent separation was realized by slanted top-bottom electrodes with the flowing cell suspension between them. Turning on the voltage deflected the cells from their initial path as determined by the streaming and thus changed their direction of flow. The separation efficiency of DEP was tested for various electrical field strengths and its performance was determined by quantitative analysis of optical and fluorescence videos. It could be shown for all size groups that the most lipid-containing cells were always subject to DEP separation and that the method is thus not only suitable for process analysis, but also for strain selection of the most productive cell lines. Frontiers Media S.A. 2022-05-23 /pmc/articles/PMC9169251/ /pubmed/35677299 http://dx.doi.org/10.3389/fbioe.2022.855035 Text en Copyright © 2022 Birkholz, Malti, Hartmann and Neubauer. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology Birkholz, Mario Malti, Danai Eleni Hartmann, Stephan Neubauer, Peter Separation of Heterotrophic Microalgae Crypthecodinium cohnii by Dielectrophoresis |
title | Separation of Heterotrophic Microalgae Crypthecodinium cohnii by Dielectrophoresis |
title_full | Separation of Heterotrophic Microalgae Crypthecodinium cohnii by Dielectrophoresis |
title_fullStr | Separation of Heterotrophic Microalgae Crypthecodinium cohnii by Dielectrophoresis |
title_full_unstemmed | Separation of Heterotrophic Microalgae Crypthecodinium cohnii by Dielectrophoresis |
title_short | Separation of Heterotrophic Microalgae Crypthecodinium cohnii by Dielectrophoresis |
title_sort | separation of heterotrophic microalgae crypthecodinium cohnii by dielectrophoresis |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9169251/ https://www.ncbi.nlm.nih.gov/pubmed/35677299 http://dx.doi.org/10.3389/fbioe.2022.855035 |
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