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Comparative Analysis of Gene Expression Patterns for Oral Epithelial Cell Functions in Periodontitis

The structure and function of epithelial cells are critical for the construction and maintenance of intact epithelial surfaces throughout the body. Beyond the mechanical barrier functions, epithelial cells have been identified as active participants in providing warning signals to the host immune an...

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Autores principales: Gonzalez, Octavio A., Kirakodu, Sreenatha, Nguyen, Linh M., Orraca, Luis, Novak, Michael J., Gonzalez-Martinez, Janis, Ebersole, Jeffrey L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9169451/
https://www.ncbi.nlm.nih.gov/pubmed/35677025
http://dx.doi.org/10.3389/froh.2022.863231
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author Gonzalez, Octavio A.
Kirakodu, Sreenatha
Nguyen, Linh M.
Orraca, Luis
Novak, Michael J.
Gonzalez-Martinez, Janis
Ebersole, Jeffrey L.
author_facet Gonzalez, Octavio A.
Kirakodu, Sreenatha
Nguyen, Linh M.
Orraca, Luis
Novak, Michael J.
Gonzalez-Martinez, Janis
Ebersole, Jeffrey L.
author_sort Gonzalez, Octavio A.
collection PubMed
description The structure and function of epithelial cells are critical for the construction and maintenance of intact epithelial surfaces throughout the body. Beyond the mechanical barrier functions, epithelial cells have been identified as active participants in providing warning signals to the host immune and inflammatory cells and in communicating various detailed information on the noxious challenge to help drive specificity in the characteristics of the host response related to health or pathologic inflammation. Rhesus monkeys were used in these studies to evaluate the gingival transcriptome for naturally occurring disease samples (GeneChip® Rhesus Macaque Genome Array) or for ligature-induced disease (GeneChip® Rhesus Gene 1.0 ST Array) to explore up to 452 annotated genes related to epithelial cell structure and functions. Animals were distributed by age into four groups: ≤ 3 years (young), 3–7 years (adolescent), 12–16 years (adult), and 18–23 years (aged). For naturally occurring disease, adult and aged periodontitis animals were used, which comprised 34 animals (14 females and 20 males). Groups of nine animals in similar age groups were included in a ligature-induced periodontitis experiment. A buccal gingival sample from either healthy or periodontitis-affected tissues were collected, and microarray analysis performed. The overall results of this investigation suggested a substantial alteration in epithelial cell functions that occurs rapidly with disease initiation. Many of these changes were prolonged throughout disease progression and generally reflect a disruption of normal cellular functions that would presage the resulting tissue destruction and clinical disease measures. Finally, clinical resolution may not signify biological resolution and represent a continued risk for disease that may require considerations for additional biologically specific interventions to best manage further disease.
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spelling pubmed-91694512022-06-07 Comparative Analysis of Gene Expression Patterns for Oral Epithelial Cell Functions in Periodontitis Gonzalez, Octavio A. Kirakodu, Sreenatha Nguyen, Linh M. Orraca, Luis Novak, Michael J. Gonzalez-Martinez, Janis Ebersole, Jeffrey L. Front Oral Health Oral Health The structure and function of epithelial cells are critical for the construction and maintenance of intact epithelial surfaces throughout the body. Beyond the mechanical barrier functions, epithelial cells have been identified as active participants in providing warning signals to the host immune and inflammatory cells and in communicating various detailed information on the noxious challenge to help drive specificity in the characteristics of the host response related to health or pathologic inflammation. Rhesus monkeys were used in these studies to evaluate the gingival transcriptome for naturally occurring disease samples (GeneChip® Rhesus Macaque Genome Array) or for ligature-induced disease (GeneChip® Rhesus Gene 1.0 ST Array) to explore up to 452 annotated genes related to epithelial cell structure and functions. Animals were distributed by age into four groups: ≤ 3 years (young), 3–7 years (adolescent), 12–16 years (adult), and 18–23 years (aged). For naturally occurring disease, adult and aged periodontitis animals were used, which comprised 34 animals (14 females and 20 males). Groups of nine animals in similar age groups were included in a ligature-induced periodontitis experiment. A buccal gingival sample from either healthy or periodontitis-affected tissues were collected, and microarray analysis performed. The overall results of this investigation suggested a substantial alteration in epithelial cell functions that occurs rapidly with disease initiation. Many of these changes were prolonged throughout disease progression and generally reflect a disruption of normal cellular functions that would presage the resulting tissue destruction and clinical disease measures. Finally, clinical resolution may not signify biological resolution and represent a continued risk for disease that may require considerations for additional biologically specific interventions to best manage further disease. Frontiers Media S.A. 2022-05-23 /pmc/articles/PMC9169451/ /pubmed/35677025 http://dx.doi.org/10.3389/froh.2022.863231 Text en Copyright © 2022 Gonzalez, Kirakodu, Nguyen, Orraca, Novak, Gonzalez-Martinez and Ebersole. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oral Health
Gonzalez, Octavio A.
Kirakodu, Sreenatha
Nguyen, Linh M.
Orraca, Luis
Novak, Michael J.
Gonzalez-Martinez, Janis
Ebersole, Jeffrey L.
Comparative Analysis of Gene Expression Patterns for Oral Epithelial Cell Functions in Periodontitis
title Comparative Analysis of Gene Expression Patterns for Oral Epithelial Cell Functions in Periodontitis
title_full Comparative Analysis of Gene Expression Patterns for Oral Epithelial Cell Functions in Periodontitis
title_fullStr Comparative Analysis of Gene Expression Patterns for Oral Epithelial Cell Functions in Periodontitis
title_full_unstemmed Comparative Analysis of Gene Expression Patterns for Oral Epithelial Cell Functions in Periodontitis
title_short Comparative Analysis of Gene Expression Patterns for Oral Epithelial Cell Functions in Periodontitis
title_sort comparative analysis of gene expression patterns for oral epithelial cell functions in periodontitis
topic Oral Health
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9169451/
https://www.ncbi.nlm.nih.gov/pubmed/35677025
http://dx.doi.org/10.3389/froh.2022.863231
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