Global fitting for high-accuracy multi-channel single-molecule localization

Multi-channel detection in single-molecule localization microscopy greatly increases information content for various biological applications. Here, we present globLoc, a graphics processing unit based global fitting algorithm with flexible PSF modeling and parameter sharing, to extract maximum infor...

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Autores principales: Li, Yiming, Shi, Wei, Liu, Sheng, Cavka, Ivana, Wu, Yu-Le, Matti, Ulf, Wu, Decheng, Koehler, Simone, Ries, Jonas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9170706/
https://www.ncbi.nlm.nih.gov/pubmed/35668089
http://dx.doi.org/10.1038/s41467-022-30719-4
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author Li, Yiming
Shi, Wei
Liu, Sheng
Cavka, Ivana
Wu, Yu-Le
Matti, Ulf
Wu, Decheng
Koehler, Simone
Ries, Jonas
author_facet Li, Yiming
Shi, Wei
Liu, Sheng
Cavka, Ivana
Wu, Yu-Le
Matti, Ulf
Wu, Decheng
Koehler, Simone
Ries, Jonas
author_sort Li, Yiming
collection PubMed
description Multi-channel detection in single-molecule localization microscopy greatly increases information content for various biological applications. Here, we present globLoc, a graphics processing unit based global fitting algorithm with flexible PSF modeling and parameter sharing, to extract maximum information from multi-channel single molecule data. As signals in multi-channel data are highly correlated, globLoc links parameters such as 3D coordinates or photon counts across channels, improving localization precision and robustness. We show, both in simulations and experiments, that global fitting can substantially improve the 3D localization precision for biplane and 4Pi single-molecule localization microscopy and color assignment for ratiometric multicolor imaging.
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spelling pubmed-91707062022-06-08 Global fitting for high-accuracy multi-channel single-molecule localization Li, Yiming Shi, Wei Liu, Sheng Cavka, Ivana Wu, Yu-Le Matti, Ulf Wu, Decheng Koehler, Simone Ries, Jonas Nat Commun Article Multi-channel detection in single-molecule localization microscopy greatly increases information content for various biological applications. Here, we present globLoc, a graphics processing unit based global fitting algorithm with flexible PSF modeling and parameter sharing, to extract maximum information from multi-channel single molecule data. As signals in multi-channel data are highly correlated, globLoc links parameters such as 3D coordinates or photon counts across channels, improving localization precision and robustness. We show, both in simulations and experiments, that global fitting can substantially improve the 3D localization precision for biplane and 4Pi single-molecule localization microscopy and color assignment for ratiometric multicolor imaging. Nature Publishing Group UK 2022-06-06 /pmc/articles/PMC9170706/ /pubmed/35668089 http://dx.doi.org/10.1038/s41467-022-30719-4 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Li, Yiming
Shi, Wei
Liu, Sheng
Cavka, Ivana
Wu, Yu-Le
Matti, Ulf
Wu, Decheng
Koehler, Simone
Ries, Jonas
Global fitting for high-accuracy multi-channel single-molecule localization
title Global fitting for high-accuracy multi-channel single-molecule localization
title_full Global fitting for high-accuracy multi-channel single-molecule localization
title_fullStr Global fitting for high-accuracy multi-channel single-molecule localization
title_full_unstemmed Global fitting for high-accuracy multi-channel single-molecule localization
title_short Global fitting for high-accuracy multi-channel single-molecule localization
title_sort global fitting for high-accuracy multi-channel single-molecule localization
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9170706/
https://www.ncbi.nlm.nih.gov/pubmed/35668089
http://dx.doi.org/10.1038/s41467-022-30719-4
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