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Coordination of siderophore gene expression among clonal cells of the bacterium Pseudomonas aeruginosa

There has been great progress in understanding how bacterial groups coordinate social actions, such as biofilm formation and public-goods secretion. Less clear is whether the seemingly coordinated group-level responses actually mirror what individual cells do. Here, we use a microscopy approach to s...

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Autores principales: Mridha, Subham, Kümmerli, Rolf
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9170778/
https://www.ncbi.nlm.nih.gov/pubmed/35668142
http://dx.doi.org/10.1038/s42003-022-03493-8
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author Mridha, Subham
Kümmerli, Rolf
author_facet Mridha, Subham
Kümmerli, Rolf
author_sort Mridha, Subham
collection PubMed
description There has been great progress in understanding how bacterial groups coordinate social actions, such as biofilm formation and public-goods secretion. Less clear is whether the seemingly coordinated group-level responses actually mirror what individual cells do. Here, we use a microscopy approach to simultaneously quantify the investment of individual cells of the bacterium Pseudomonas aeruginosa into two public goods, the siderophores pyochelin and pyoverdine. Using gene expression as a proxy for investment, we initially observe no coordination but high heterogeneity and bimodality in siderophore investment across cells. With increasing cell density, gene expression becomes more homogenized across cells, accompanied by a moderate shift from pyochelin to pyoverdine expression. We find positive associations in the expression of pyochelin and pyoverdine genes across cells, with cell-to-cell variation correlating with cellular metabolic states. Our work suggests that siderophore-mediated signalling aligns behaviour of individuals over time and spurs a coordinated three-phase siderophore investment cycle.
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spelling pubmed-91707782022-06-08 Coordination of siderophore gene expression among clonal cells of the bacterium Pseudomonas aeruginosa Mridha, Subham Kümmerli, Rolf Commun Biol Article There has been great progress in understanding how bacterial groups coordinate social actions, such as biofilm formation and public-goods secretion. Less clear is whether the seemingly coordinated group-level responses actually mirror what individual cells do. Here, we use a microscopy approach to simultaneously quantify the investment of individual cells of the bacterium Pseudomonas aeruginosa into two public goods, the siderophores pyochelin and pyoverdine. Using gene expression as a proxy for investment, we initially observe no coordination but high heterogeneity and bimodality in siderophore investment across cells. With increasing cell density, gene expression becomes more homogenized across cells, accompanied by a moderate shift from pyochelin to pyoverdine expression. We find positive associations in the expression of pyochelin and pyoverdine genes across cells, with cell-to-cell variation correlating with cellular metabolic states. Our work suggests that siderophore-mediated signalling aligns behaviour of individuals over time and spurs a coordinated three-phase siderophore investment cycle. Nature Publishing Group UK 2022-06-06 /pmc/articles/PMC9170778/ /pubmed/35668142 http://dx.doi.org/10.1038/s42003-022-03493-8 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Mridha, Subham
Kümmerli, Rolf
Coordination of siderophore gene expression among clonal cells of the bacterium Pseudomonas aeruginosa
title Coordination of siderophore gene expression among clonal cells of the bacterium Pseudomonas aeruginosa
title_full Coordination of siderophore gene expression among clonal cells of the bacterium Pseudomonas aeruginosa
title_fullStr Coordination of siderophore gene expression among clonal cells of the bacterium Pseudomonas aeruginosa
title_full_unstemmed Coordination of siderophore gene expression among clonal cells of the bacterium Pseudomonas aeruginosa
title_short Coordination of siderophore gene expression among clonal cells of the bacterium Pseudomonas aeruginosa
title_sort coordination of siderophore gene expression among clonal cells of the bacterium pseudomonas aeruginosa
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9170778/
https://www.ncbi.nlm.nih.gov/pubmed/35668142
http://dx.doi.org/10.1038/s42003-022-03493-8
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