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Identification and genetic characterization of bovine hepacivirus in China: A large scale epidemiological study

Bovine hepacivirus (BovHepV) is a novel virus that was recently discovered in Ghana and Germany in 2015. Until now, this virus has been identified in cattle population worldwide and is classified into subtypes A–G. To fully understand the epidemic situation and genetic characteristic of BovHepV in C...

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Autores principales: Lu, Gang, Chen, Chaoxi, Shao, Ran, Zhang, Juan, Li, Jinghao, Cai, Siqi, Zhong, Lintao, Lai, Zhiying, Ou, Jiajun, Yin, Xin, Zhang, Guihong, Li, Shoujun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wuhan Institute of Virology, Chinese Academy of Sciences 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9170970/
https://www.ncbi.nlm.nih.gov/pubmed/35537981
http://dx.doi.org/10.1016/j.virs.2022.02.003
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author Lu, Gang
Chen, Chaoxi
Shao, Ran
Zhang, Juan
Li, Jinghao
Cai, Siqi
Zhong, Lintao
Lai, Zhiying
Ou, Jiajun
Yin, Xin
Zhang, Guihong
Li, Shoujun
author_facet Lu, Gang
Chen, Chaoxi
Shao, Ran
Zhang, Juan
Li, Jinghao
Cai, Siqi
Zhong, Lintao
Lai, Zhiying
Ou, Jiajun
Yin, Xin
Zhang, Guihong
Li, Shoujun
author_sort Lu, Gang
collection PubMed
description Bovine hepacivirus (BovHepV) is a novel virus that was recently discovered in Ghana and Germany in 2015. Until now, this virus has been identified in cattle population worldwide and is classified into subtypes A–G. To fully understand the epidemic situation and genetic characteristic of BovHepV in China, a total of 612 cattle serum samples were collected from 20 farms in seven provinces and municipality in China between 2018 and 2020 and were tested for the presence of BovHepV RNA via semi-nested PCR. The results demonstrated that 49 (8.0%) samples were BovHepV RNA-positive. It is noted that BovHepV infection in yak was confirmed for the first time. BovHepV was detected in all the seven provinces, with the positive rate ranging from 3.1% to 13.3%, which indicates a wide geographical distribution pattern of BovHepV in China. Sequencing results revealed that 5′ UTR of the 49 field BovHepV strains have a nucleotide similarity of 96.3%–100% between each other and 93.9%–100% with previously reported BovHepV strains. In addition, genetic analysis identified five critical nucleotide sites in 5′ UTR to distinguish different subtypes, which was further verified by genomic sequencing and nucleotide similarity analysis. All the 49 Chinese field BovHepV strains were classified into subtype G and this subtype is only determined in cattle in China currently. This study will provide insights for us to better understand the epidemiology and genetic diversity of BovHepV.
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spelling pubmed-91709702022-06-08 Identification and genetic characterization of bovine hepacivirus in China: A large scale epidemiological study Lu, Gang Chen, Chaoxi Shao, Ran Zhang, Juan Li, Jinghao Cai, Siqi Zhong, Lintao Lai, Zhiying Ou, Jiajun Yin, Xin Zhang, Guihong Li, Shoujun Virol Sin Research Article Bovine hepacivirus (BovHepV) is a novel virus that was recently discovered in Ghana and Germany in 2015. Until now, this virus has been identified in cattle population worldwide and is classified into subtypes A–G. To fully understand the epidemic situation and genetic characteristic of BovHepV in China, a total of 612 cattle serum samples were collected from 20 farms in seven provinces and municipality in China between 2018 and 2020 and were tested for the presence of BovHepV RNA via semi-nested PCR. The results demonstrated that 49 (8.0%) samples were BovHepV RNA-positive. It is noted that BovHepV infection in yak was confirmed for the first time. BovHepV was detected in all the seven provinces, with the positive rate ranging from 3.1% to 13.3%, which indicates a wide geographical distribution pattern of BovHepV in China. Sequencing results revealed that 5′ UTR of the 49 field BovHepV strains have a nucleotide similarity of 96.3%–100% between each other and 93.9%–100% with previously reported BovHepV strains. In addition, genetic analysis identified five critical nucleotide sites in 5′ UTR to distinguish different subtypes, which was further verified by genomic sequencing and nucleotide similarity analysis. All the 49 Chinese field BovHepV strains were classified into subtype G and this subtype is only determined in cattle in China currently. This study will provide insights for us to better understand the epidemiology and genetic diversity of BovHepV. Wuhan Institute of Virology, Chinese Academy of Sciences 2022-02-12 /pmc/articles/PMC9170970/ /pubmed/35537981 http://dx.doi.org/10.1016/j.virs.2022.02.003 Text en © 2022 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Research Article
Lu, Gang
Chen, Chaoxi
Shao, Ran
Zhang, Juan
Li, Jinghao
Cai, Siqi
Zhong, Lintao
Lai, Zhiying
Ou, Jiajun
Yin, Xin
Zhang, Guihong
Li, Shoujun
Identification and genetic characterization of bovine hepacivirus in China: A large scale epidemiological study
title Identification and genetic characterization of bovine hepacivirus in China: A large scale epidemiological study
title_full Identification and genetic characterization of bovine hepacivirus in China: A large scale epidemiological study
title_fullStr Identification and genetic characterization of bovine hepacivirus in China: A large scale epidemiological study
title_full_unstemmed Identification and genetic characterization of bovine hepacivirus in China: A large scale epidemiological study
title_short Identification and genetic characterization of bovine hepacivirus in China: A large scale epidemiological study
title_sort identification and genetic characterization of bovine hepacivirus in china: a large scale epidemiological study
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9170970/
https://www.ncbi.nlm.nih.gov/pubmed/35537981
http://dx.doi.org/10.1016/j.virs.2022.02.003
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