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Common Considerations for Genotoxicity Assessment of Nanomaterials

Genotoxicity testing is performed to determine potential hazard of a chemical or agent for direct or indirect DNA interaction. Testing may be a surrogate for assessment of heritable genetic risk or carcinogenic risk. Testing of nanomaterials (NM) for hazard identification is generally understood to...

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Autores principales: Elespuru, Rosalie K., Doak, Shareen H., Collins, Andrew R., Dusinska, Maria, Pfuhler, Stefan, Manjanatha, Mugimane, Cardoso, Renato, Chen, Connie L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9171035/
https://www.ncbi.nlm.nih.gov/pubmed/35686044
http://dx.doi.org/10.3389/ftox.2022.859122
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author Elespuru, Rosalie K.
Doak, Shareen H.
Collins, Andrew R.
Dusinska, Maria
Pfuhler, Stefan
Manjanatha, Mugimane
Cardoso, Renato
Chen, Connie L.
author_facet Elespuru, Rosalie K.
Doak, Shareen H.
Collins, Andrew R.
Dusinska, Maria
Pfuhler, Stefan
Manjanatha, Mugimane
Cardoso, Renato
Chen, Connie L.
author_sort Elespuru, Rosalie K.
collection PubMed
description Genotoxicity testing is performed to determine potential hazard of a chemical or agent for direct or indirect DNA interaction. Testing may be a surrogate for assessment of heritable genetic risk or carcinogenic risk. Testing of nanomaterials (NM) for hazard identification is generally understood to require a departure from normal testing procedures found in international standards and guidelines. A critique of the genotoxicity literature in Elespuru et al., 2018, reinforced evidence of problems with genotoxicity assessment of nanomaterials (NM) noted by many previously. A follow-up to the critique of problems (what is wrong) is a series of methods papers in this journal designed to provide practical information on what is appropriate (right) in the performance of genotoxicity assays altered for NM assessment. In this “Common Considerations” paper, general considerations are addressed, including NM characterization, sample preparation, dosing choice, exposure assessment (uptake) and data analysis that are applicable to any NM genotoxicity assessment. Recommended methods for specific assays are presented in a series of additional papers in this special issue of the journal devoted to toxicology methods for assessment of nanomaterials: the In vitro Micronucleus Assay, TK Mutagenicity assays, and the In vivo Comet Assay. In this context, NM are considered generally as insoluble particles or test articles in the nanometer size range that present difficulties in assessment using techniques described in standards such as OECD guidelines.
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spelling pubmed-91710352022-06-08 Common Considerations for Genotoxicity Assessment of Nanomaterials Elespuru, Rosalie K. Doak, Shareen H. Collins, Andrew R. Dusinska, Maria Pfuhler, Stefan Manjanatha, Mugimane Cardoso, Renato Chen, Connie L. Front Toxicol Toxicology Genotoxicity testing is performed to determine potential hazard of a chemical or agent for direct or indirect DNA interaction. Testing may be a surrogate for assessment of heritable genetic risk or carcinogenic risk. Testing of nanomaterials (NM) for hazard identification is generally understood to require a departure from normal testing procedures found in international standards and guidelines. A critique of the genotoxicity literature in Elespuru et al., 2018, reinforced evidence of problems with genotoxicity assessment of nanomaterials (NM) noted by many previously. A follow-up to the critique of problems (what is wrong) is a series of methods papers in this journal designed to provide practical information on what is appropriate (right) in the performance of genotoxicity assays altered for NM assessment. In this “Common Considerations” paper, general considerations are addressed, including NM characterization, sample preparation, dosing choice, exposure assessment (uptake) and data analysis that are applicable to any NM genotoxicity assessment. Recommended methods for specific assays are presented in a series of additional papers in this special issue of the journal devoted to toxicology methods for assessment of nanomaterials: the In vitro Micronucleus Assay, TK Mutagenicity assays, and the In vivo Comet Assay. In this context, NM are considered generally as insoluble particles or test articles in the nanometer size range that present difficulties in assessment using techniques described in standards such as OECD guidelines. Frontiers Media S.A. 2022-05-24 /pmc/articles/PMC9171035/ /pubmed/35686044 http://dx.doi.org/10.3389/ftox.2022.859122 Text en Copyright © 2022 Elespuru, Doak, Collins, Dusinska, Pfuhler, Manjanatha, Cardoso and Chen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Toxicology
Elespuru, Rosalie K.
Doak, Shareen H.
Collins, Andrew R.
Dusinska, Maria
Pfuhler, Stefan
Manjanatha, Mugimane
Cardoso, Renato
Chen, Connie L.
Common Considerations for Genotoxicity Assessment of Nanomaterials
title Common Considerations for Genotoxicity Assessment of Nanomaterials
title_full Common Considerations for Genotoxicity Assessment of Nanomaterials
title_fullStr Common Considerations for Genotoxicity Assessment of Nanomaterials
title_full_unstemmed Common Considerations for Genotoxicity Assessment of Nanomaterials
title_short Common Considerations for Genotoxicity Assessment of Nanomaterials
title_sort common considerations for genotoxicity assessment of nanomaterials
topic Toxicology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9171035/
https://www.ncbi.nlm.nih.gov/pubmed/35686044
http://dx.doi.org/10.3389/ftox.2022.859122
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