Cargando…

Three-Dimensional Cartilage Regeneration Using Engineered Cartilage Gel With a 3D-Printed Polycaprolactone Framework

The feasibility of the three-dimensional (3D) cartilage regeneration technology based on the “steel (framework)-reinforced concrete (engineered cartilage gel, ECG)” concept has been verified in large animals using a decalcified bone matrix (DBM) as the framework. However, the instability of the sour...

Descripción completa

Detalles Bibliográficos
Autores principales: Wu, Gaoyang, Lu, Lixing, Ci, Zheng, Wang, Yahui, Shi, Runjie, Zhou, Guangdong, Li, Shengli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9171075/
https://www.ncbi.nlm.nih.gov/pubmed/35685090
http://dx.doi.org/10.3389/fbioe.2022.871508
_version_ 1784721581126189056
author Wu, Gaoyang
Lu, Lixing
Ci, Zheng
Wang, Yahui
Shi, Runjie
Zhou, Guangdong
Li, Shengli
author_facet Wu, Gaoyang
Lu, Lixing
Ci, Zheng
Wang, Yahui
Shi, Runjie
Zhou, Guangdong
Li, Shengli
author_sort Wu, Gaoyang
collection PubMed
description The feasibility of the three-dimensional (3D) cartilage regeneration technology based on the “steel (framework)-reinforced concrete (engineered cartilage gel, ECG)” concept has been verified in large animals using a decalcified bone matrix (DBM) as the framework. However, the instability of the source, large sample variation, and lack of control over the 3D shape of DBM have greatly hindered clinical translation of this technology. To optimize cartilage regeneration using the ECG–framework model, the current study explores the feasibility of replacing the DBM framework with a 3D-printed polycaprolactone (PCL) framework. The PCL framework showed good biocompatibility with ECG and achieved a high ECG loading efficiency, similar to that of the DBM framework. Furthermore, PCL-ECG constructs caused a milder inflammatory response in vivo than that induced by DBM-ECG constructs, which was further supported by an in vitro macrophage activation experiment. Notably, the PCL-ECG constructs successfully regenerated mature cartilage and essentially maintained their original shape throughout 8 weeks of subcutaneous implantation. Quantitative analysis revealed that the GAG and total collagen contents of the regenerated cartilage in the PCL-ECG group were significantly higher than those in the DBM-ECG group. The results indicated that the 3D-printed PCL framework—a clinically approved biomaterial with multiple advantages including customizable shape design, mechanical strength control, and standardized production—can serve as an excellent framework for supporting the 3D cartilage regeneration of ECG. This provides a feasible novel strategy for the clinical translation of ECG-based 3D cartilage regeneration.
format Online
Article
Text
id pubmed-9171075
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-91710752022-06-08 Three-Dimensional Cartilage Regeneration Using Engineered Cartilage Gel With a 3D-Printed Polycaprolactone Framework Wu, Gaoyang Lu, Lixing Ci, Zheng Wang, Yahui Shi, Runjie Zhou, Guangdong Li, Shengli Front Bioeng Biotechnol Bioengineering and Biotechnology The feasibility of the three-dimensional (3D) cartilage regeneration technology based on the “steel (framework)-reinforced concrete (engineered cartilage gel, ECG)” concept has been verified in large animals using a decalcified bone matrix (DBM) as the framework. However, the instability of the source, large sample variation, and lack of control over the 3D shape of DBM have greatly hindered clinical translation of this technology. To optimize cartilage regeneration using the ECG–framework model, the current study explores the feasibility of replacing the DBM framework with a 3D-printed polycaprolactone (PCL) framework. The PCL framework showed good biocompatibility with ECG and achieved a high ECG loading efficiency, similar to that of the DBM framework. Furthermore, PCL-ECG constructs caused a milder inflammatory response in vivo than that induced by DBM-ECG constructs, which was further supported by an in vitro macrophage activation experiment. Notably, the PCL-ECG constructs successfully regenerated mature cartilage and essentially maintained their original shape throughout 8 weeks of subcutaneous implantation. Quantitative analysis revealed that the GAG and total collagen contents of the regenerated cartilage in the PCL-ECG group were significantly higher than those in the DBM-ECG group. The results indicated that the 3D-printed PCL framework—a clinically approved biomaterial with multiple advantages including customizable shape design, mechanical strength control, and standardized production—can serve as an excellent framework for supporting the 3D cartilage regeneration of ECG. This provides a feasible novel strategy for the clinical translation of ECG-based 3D cartilage regeneration. Frontiers Media S.A. 2022-05-24 /pmc/articles/PMC9171075/ /pubmed/35685090 http://dx.doi.org/10.3389/fbioe.2022.871508 Text en Copyright © 2022 Wu, Lu, Ci, Wang, Shi, Zhou and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Wu, Gaoyang
Lu, Lixing
Ci, Zheng
Wang, Yahui
Shi, Runjie
Zhou, Guangdong
Li, Shengli
Three-Dimensional Cartilage Regeneration Using Engineered Cartilage Gel With a 3D-Printed Polycaprolactone Framework
title Three-Dimensional Cartilage Regeneration Using Engineered Cartilage Gel With a 3D-Printed Polycaprolactone Framework
title_full Three-Dimensional Cartilage Regeneration Using Engineered Cartilage Gel With a 3D-Printed Polycaprolactone Framework
title_fullStr Three-Dimensional Cartilage Regeneration Using Engineered Cartilage Gel With a 3D-Printed Polycaprolactone Framework
title_full_unstemmed Three-Dimensional Cartilage Regeneration Using Engineered Cartilage Gel With a 3D-Printed Polycaprolactone Framework
title_short Three-Dimensional Cartilage Regeneration Using Engineered Cartilage Gel With a 3D-Printed Polycaprolactone Framework
title_sort three-dimensional cartilage regeneration using engineered cartilage gel with a 3d-printed polycaprolactone framework
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9171075/
https://www.ncbi.nlm.nih.gov/pubmed/35685090
http://dx.doi.org/10.3389/fbioe.2022.871508
work_keys_str_mv AT wugaoyang threedimensionalcartilageregenerationusingengineeredcartilagegelwitha3dprintedpolycaprolactoneframework
AT lulixing threedimensionalcartilageregenerationusingengineeredcartilagegelwitha3dprintedpolycaprolactoneframework
AT cizheng threedimensionalcartilageregenerationusingengineeredcartilagegelwitha3dprintedpolycaprolactoneframework
AT wangyahui threedimensionalcartilageregenerationusingengineeredcartilagegelwitha3dprintedpolycaprolactoneframework
AT shirunjie threedimensionalcartilageregenerationusingengineeredcartilagegelwitha3dprintedpolycaprolactoneframework
AT zhouguangdong threedimensionalcartilageregenerationusingengineeredcartilagegelwitha3dprintedpolycaprolactoneframework
AT lishengli threedimensionalcartilageregenerationusingengineeredcartilagegelwitha3dprintedpolycaprolactoneframework