The C. elegans Casein Kinase II is associated with meiotic DNA in fertilized oocytes

By using CRISPR/Cas9 genome-editing, we have generated epitope-tagged KIN-3 and KIN-10 expressing strains at the endogenous C-terminal loci in Caenorhabditis elegans . We observed that both the catalytic (KIN-3::V5) and regulatory (KIN-10::2xMyc) subunits of the Casein Kinase II (CK2) holoenzyme com...

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Detalles Bibliográficos
Autores principales: Yim, Nahyun, Medley, Jeffrey C., Song, Mi Hye
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2022
Materias:
New Finding
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9171554/
https://www.ncbi.nlm.nih.gov/pubmed/35685274
http://dx.doi.org/10.17912/micropub.biology.000583
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author Yim, Nahyun
Medley, Jeffrey C.
Song, Mi Hye
author_facet Yim, Nahyun
Medley, Jeffrey C.
Song, Mi Hye
author_sort Yim, Nahyun
collection PubMed
description By using CRISPR/Cas9 genome-editing, we have generated epitope-tagged KIN-3 and KIN-10 expressing strains at the endogenous C-terminal loci in Caenorhabditis elegans . We observed that both the catalytic (KIN-3::V5) and regulatory (KIN-10::2xMyc) subunits of the Casein Kinase II (CK2) holoenzyme complex are associated with meiotic DNA, enriched in the midvalent rings during meiotic divisions in fertilized C. elegans oocytes.
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spelling pubmed-91715542022-06-08 The C. elegans Casein Kinase II is associated with meiotic DNA in fertilized oocytes Yim, Nahyun Medley, Jeffrey C. Song, Mi Hye MicroPubl Biol New Finding By using CRISPR/Cas9 genome-editing, we have generated epitope-tagged KIN-3 and KIN-10 expressing strains at the endogenous C-terminal loci in Caenorhabditis elegans . We observed that both the catalytic (KIN-3::V5) and regulatory (KIN-10::2xMyc) subunits of the Casein Kinase II (CK2) holoenzyme complex are associated with meiotic DNA, enriched in the midvalent rings during meiotic divisions in fertilized C. elegans oocytes. Caltech Library 2022-06-06 /pmc/articles/PMC9171554/ /pubmed/35685274 http://dx.doi.org/10.17912/micropub.biology.000583 Text en Copyright: © 2022 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle New Finding
Yim, Nahyun
Medley, Jeffrey C.
Song, Mi Hye
The C. elegans Casein Kinase II is associated with meiotic DNA in fertilized oocytes
title The C. elegans Casein Kinase II is associated with meiotic DNA in fertilized oocytes
title_full The C. elegans Casein Kinase II is associated with meiotic DNA in fertilized oocytes
title_fullStr The C. elegans Casein Kinase II is associated with meiotic DNA in fertilized oocytes
title_full_unstemmed The C. elegans Casein Kinase II is associated with meiotic DNA in fertilized oocytes
title_short The C. elegans Casein Kinase II is associated with meiotic DNA in fertilized oocytes
title_sort the c. elegans casein kinase ii is associated with meiotic dna in fertilized oocytes
topic New Finding
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9171554/
https://www.ncbi.nlm.nih.gov/pubmed/35685274
http://dx.doi.org/10.17912/micropub.biology.000583
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