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Genetic Modification of Bergera koenigii for Expression of the Bacterial Pesticidal Protein Cry1Ba1

The curry leaf tree, Bergera koenigii, is highly attractive to the Asian citrus psyllid, Diaphorina citri, which vectors the bacterial causative agent of citrus greening or huanglongbing disease. This disease has decimated citrus production in Florida and in other citrus-producing countries. As D. c...

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Autores principales: Ravanfar, Seyed Ali, Achor, Diann S., Killiny, Nabil, Shilts, Turksen, Chen, Yuting, El-Mohtar, Choaa, Stelinski, Lukasz L., Bonning, Bryony C., Orbović, Vladimir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9171844/
https://www.ncbi.nlm.nih.gov/pubmed/35685021
http://dx.doi.org/10.3389/fpls.2022.899624
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author Ravanfar, Seyed Ali
Achor, Diann S.
Killiny, Nabil
Shilts, Turksen
Chen, Yuting
El-Mohtar, Choaa
Stelinski, Lukasz L.
Bonning, Bryony C.
Orbović, Vladimir
author_facet Ravanfar, Seyed Ali
Achor, Diann S.
Killiny, Nabil
Shilts, Turksen
Chen, Yuting
El-Mohtar, Choaa
Stelinski, Lukasz L.
Bonning, Bryony C.
Orbović, Vladimir
author_sort Ravanfar, Seyed Ali
collection PubMed
description The curry leaf tree, Bergera koenigii, is highly attractive to the Asian citrus psyllid, Diaphorina citri, which vectors the bacterial causative agent of citrus greening or huanglongbing disease. This disease has decimated citrus production in Florida and in other citrus-producing countries. As D. citri exhibits high affinity for feeding on young leaves of B. koenigii, transgenic B. koenigii expressing bacteria-derived pesticidal proteins such as Cry1Ba1 have potential for D. citri management when planted in or adjacent to citrus groves. Importantly, the plant pathogenic bacterium that causes citrus greening does not replicate in B. koenigii. Transgenic plants of B. koenigii were produced by insertion of the gene encoding the active core of the pesticidal protein Cry1Ba1 derived from Bacillus thuringiensis. The transformation success rate was low relative to that of other citrus, at 0.89%. T-DNA integration into the genome and cry1ba1 transcription in transgenic plants were confirmed. Transgenic plants expressing Cry1Ba1 differed from wild-type plants, differed in photosynthesis parameters and hormone levels in some instances, and a marked delay in wilting of detached leaves. The gut epithelium of D. citri fed on transgenic plants was severely damaged, consistent with Cry1Ba1-mediated pore formation, confirming expression of the pesticidal protein by transgenic B. koenigii. These results demonstrate that transgenic B. koenigii expressing bacteria-derived pesticidal proteins can be produced for potential use as trap plants for suppression of D. citri populations toward protection of citrus groves from citrus greening.
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spelling pubmed-91718442022-06-08 Genetic Modification of Bergera koenigii for Expression of the Bacterial Pesticidal Protein Cry1Ba1 Ravanfar, Seyed Ali Achor, Diann S. Killiny, Nabil Shilts, Turksen Chen, Yuting El-Mohtar, Choaa Stelinski, Lukasz L. Bonning, Bryony C. Orbović, Vladimir Front Plant Sci Plant Science The curry leaf tree, Bergera koenigii, is highly attractive to the Asian citrus psyllid, Diaphorina citri, which vectors the bacterial causative agent of citrus greening or huanglongbing disease. This disease has decimated citrus production in Florida and in other citrus-producing countries. As D. citri exhibits high affinity for feeding on young leaves of B. koenigii, transgenic B. koenigii expressing bacteria-derived pesticidal proteins such as Cry1Ba1 have potential for D. citri management when planted in or adjacent to citrus groves. Importantly, the plant pathogenic bacterium that causes citrus greening does not replicate in B. koenigii. Transgenic plants of B. koenigii were produced by insertion of the gene encoding the active core of the pesticidal protein Cry1Ba1 derived from Bacillus thuringiensis. The transformation success rate was low relative to that of other citrus, at 0.89%. T-DNA integration into the genome and cry1ba1 transcription in transgenic plants were confirmed. Transgenic plants expressing Cry1Ba1 differed from wild-type plants, differed in photosynthesis parameters and hormone levels in some instances, and a marked delay in wilting of detached leaves. The gut epithelium of D. citri fed on transgenic plants was severely damaged, consistent with Cry1Ba1-mediated pore formation, confirming expression of the pesticidal protein by transgenic B. koenigii. These results demonstrate that transgenic B. koenigii expressing bacteria-derived pesticidal proteins can be produced for potential use as trap plants for suppression of D. citri populations toward protection of citrus groves from citrus greening. Frontiers Media S.A. 2022-05-24 /pmc/articles/PMC9171844/ /pubmed/35685021 http://dx.doi.org/10.3389/fpls.2022.899624 Text en Copyright © 2022 Ravanfar, Achor, Killiny, Shilts, Chen, El-Mohtar, Stelinski, Bonning and Orbović. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Ravanfar, Seyed Ali
Achor, Diann S.
Killiny, Nabil
Shilts, Turksen
Chen, Yuting
El-Mohtar, Choaa
Stelinski, Lukasz L.
Bonning, Bryony C.
Orbović, Vladimir
Genetic Modification of Bergera koenigii for Expression of the Bacterial Pesticidal Protein Cry1Ba1
title Genetic Modification of Bergera koenigii for Expression of the Bacterial Pesticidal Protein Cry1Ba1
title_full Genetic Modification of Bergera koenigii for Expression of the Bacterial Pesticidal Protein Cry1Ba1
title_fullStr Genetic Modification of Bergera koenigii for Expression of the Bacterial Pesticidal Protein Cry1Ba1
title_full_unstemmed Genetic Modification of Bergera koenigii for Expression of the Bacterial Pesticidal Protein Cry1Ba1
title_short Genetic Modification of Bergera koenigii for Expression of the Bacterial Pesticidal Protein Cry1Ba1
title_sort genetic modification of bergera koenigii for expression of the bacterial pesticidal protein cry1ba1
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9171844/
https://www.ncbi.nlm.nih.gov/pubmed/35685021
http://dx.doi.org/10.3389/fpls.2022.899624
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