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Not Only Systemin: Prosystemin Harbors Other Active Regions Able to Protect Tomato Plants
Prosystemin is a 200-amino acid precursor expressed in Solanaceae plants which releases at the C-terminal part a peptidic hormone called Systemin in response to wounding and herbivore attack. We recently showed that Prosystemin is not only a mere scaffold of Systemin but, even when deprived of Syste...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9173717/ https://www.ncbi.nlm.nih.gov/pubmed/35685017 http://dx.doi.org/10.3389/fpls.2022.887674 |
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author | Molisso, Donata Coppola, Mariangela Buonanno, Martina Di Lelio, Ilaria Aprile, Anna Maria Langella, Emma Rigano, Maria Manuela Francesca, Silvana Chiaiese, Pasquale Palmieri, Gianna Tatè, Rosarita Sinno, Martina Barra, Eleonora Becchimanzi, Andrea Monti, Simona Maria Pennacchio, Francesco Rao, Rosa |
author_facet | Molisso, Donata Coppola, Mariangela Buonanno, Martina Di Lelio, Ilaria Aprile, Anna Maria Langella, Emma Rigano, Maria Manuela Francesca, Silvana Chiaiese, Pasquale Palmieri, Gianna Tatè, Rosarita Sinno, Martina Barra, Eleonora Becchimanzi, Andrea Monti, Simona Maria Pennacchio, Francesco Rao, Rosa |
author_sort | Molisso, Donata |
collection | PubMed |
description | Prosystemin is a 200-amino acid precursor expressed in Solanaceae plants which releases at the C-terminal part a peptidic hormone called Systemin in response to wounding and herbivore attack. We recently showed that Prosystemin is not only a mere scaffold of Systemin but, even when deprived of Systemin, is biologically active. These results, combined with recent discoveries that Prosystemin is an intrinsically disordered protein containing disordered regions within its sequence, prompted us to investigate the N-terminal portions of the precursor, which contribute to the greatest disorder within the sequence. To this aim, PS1-70 and PS1-120 were designed, produced, and structurally and functionally characterized. Both the fragments, which maintained their intrinsic disorder, were able to induce defense-related genes and to protect tomato plants against Botrytis cinerea and Spodoptera littoralis larvae. Intriguingly, the biological activity of each of the two N-terminal fragments and of Systemin is similar but not quite the same and does not show any toxicity on experimental non-targets considered. These regions account for different anti-stress activities conferred to tomato plants by their overexpression. The two N-terminal fragments identified in this study may represent new promising tools for sustainable crop protection. |
format | Online Article Text |
id | pubmed-9173717 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-91737172022-06-08 Not Only Systemin: Prosystemin Harbors Other Active Regions Able to Protect Tomato Plants Molisso, Donata Coppola, Mariangela Buonanno, Martina Di Lelio, Ilaria Aprile, Anna Maria Langella, Emma Rigano, Maria Manuela Francesca, Silvana Chiaiese, Pasquale Palmieri, Gianna Tatè, Rosarita Sinno, Martina Barra, Eleonora Becchimanzi, Andrea Monti, Simona Maria Pennacchio, Francesco Rao, Rosa Front Plant Sci Plant Science Prosystemin is a 200-amino acid precursor expressed in Solanaceae plants which releases at the C-terminal part a peptidic hormone called Systemin in response to wounding and herbivore attack. We recently showed that Prosystemin is not only a mere scaffold of Systemin but, even when deprived of Systemin, is biologically active. These results, combined with recent discoveries that Prosystemin is an intrinsically disordered protein containing disordered regions within its sequence, prompted us to investigate the N-terminal portions of the precursor, which contribute to the greatest disorder within the sequence. To this aim, PS1-70 and PS1-120 were designed, produced, and structurally and functionally characterized. Both the fragments, which maintained their intrinsic disorder, were able to induce defense-related genes and to protect tomato plants against Botrytis cinerea and Spodoptera littoralis larvae. Intriguingly, the biological activity of each of the two N-terminal fragments and of Systemin is similar but not quite the same and does not show any toxicity on experimental non-targets considered. These regions account for different anti-stress activities conferred to tomato plants by their overexpression. The two N-terminal fragments identified in this study may represent new promising tools for sustainable crop protection. Frontiers Media S.A. 2022-05-24 /pmc/articles/PMC9173717/ /pubmed/35685017 http://dx.doi.org/10.3389/fpls.2022.887674 Text en Copyright © 2022 Molisso, Coppola, Buonanno, Di Lelio, Aprile, Langella, Rigano, Francesca, Chiaiese, Palmieri, Tatè, Sinno, Barra, Becchimanzi, Monti, Pennacchio and Rao. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Molisso, Donata Coppola, Mariangela Buonanno, Martina Di Lelio, Ilaria Aprile, Anna Maria Langella, Emma Rigano, Maria Manuela Francesca, Silvana Chiaiese, Pasquale Palmieri, Gianna Tatè, Rosarita Sinno, Martina Barra, Eleonora Becchimanzi, Andrea Monti, Simona Maria Pennacchio, Francesco Rao, Rosa Not Only Systemin: Prosystemin Harbors Other Active Regions Able to Protect Tomato Plants |
title | Not Only Systemin: Prosystemin Harbors Other Active Regions Able to Protect Tomato Plants |
title_full | Not Only Systemin: Prosystemin Harbors Other Active Regions Able to Protect Tomato Plants |
title_fullStr | Not Only Systemin: Prosystemin Harbors Other Active Regions Able to Protect Tomato Plants |
title_full_unstemmed | Not Only Systemin: Prosystemin Harbors Other Active Regions Able to Protect Tomato Plants |
title_short | Not Only Systemin: Prosystemin Harbors Other Active Regions Able to Protect Tomato Plants |
title_sort | not only systemin: prosystemin harbors other active regions able to protect tomato plants |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9173717/ https://www.ncbi.nlm.nih.gov/pubmed/35685017 http://dx.doi.org/10.3389/fpls.2022.887674 |
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