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Epigenetic-structural changes in X chromosomes promote Xic pairing during early differentiation of mouse embryonic stem cells
X chromosome inactivation center (Xic) pairing occurs during the differentiation of embryonic stem (ES) cells from female mouse embryos, and is related to X chromosome inactivation, the circadian clock, intra-nucleus architecture, and metabolism. However, the mechanisms underlying the identification...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Biophysical Society of Japan
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9174021/ https://www.ncbi.nlm.nih.gov/pubmed/35797402 http://dx.doi.org/10.2142/biophysico.bppb-v19.0018 |
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author | Komoto, Tetsushi Fujii, Masashi Awazu, Akinori |
author_facet | Komoto, Tetsushi Fujii, Masashi Awazu, Akinori |
author_sort | Komoto, Tetsushi |
collection | PubMed |
description | X chromosome inactivation center (Xic) pairing occurs during the differentiation of embryonic stem (ES) cells from female mouse embryos, and is related to X chromosome inactivation, the circadian clock, intra-nucleus architecture, and metabolism. However, the mechanisms underlying the identification and approach of X chromosome pairs in the crowded nucleus are unclear. To elucidate the driving force of Xic pairing, we developed a coarse-grained molecular dynamics model of intranuclear chromosomes in ES cells and in cells 2 days after the onset of differentiation (2-day cells) by considering intrachromosomal epigenetic-structural feature-dependent mechanics. The analysis of the experimental data showed that X-chromosomes exhibit the rearrangement of their distributions of open/closed chromatin regions on their surfaces during cell differentiation. By simulating models where the excluded volume effects of closed chromatin regions are stronger than those of open chromatin regions, such rearrangement of open/closed chromatin regions on X-chromosome surfaces promoted the mutual approach of the Xic pair. These findings suggested that local intrachromosomal epigenetic features may contribute to the regulation of cell species-dependent differences in intranuclear architecture. |
format | Online Article Text |
id | pubmed-9174021 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | The Biophysical Society of Japan |
record_format | MEDLINE/PubMed |
spelling | pubmed-91740212022-07-05 Epigenetic-structural changes in X chromosomes promote Xic pairing during early differentiation of mouse embryonic stem cells Komoto, Tetsushi Fujii, Masashi Awazu, Akinori Biophys Physicobiol Regular Article X chromosome inactivation center (Xic) pairing occurs during the differentiation of embryonic stem (ES) cells from female mouse embryos, and is related to X chromosome inactivation, the circadian clock, intra-nucleus architecture, and metabolism. However, the mechanisms underlying the identification and approach of X chromosome pairs in the crowded nucleus are unclear. To elucidate the driving force of Xic pairing, we developed a coarse-grained molecular dynamics model of intranuclear chromosomes in ES cells and in cells 2 days after the onset of differentiation (2-day cells) by considering intrachromosomal epigenetic-structural feature-dependent mechanics. The analysis of the experimental data showed that X-chromosomes exhibit the rearrangement of their distributions of open/closed chromatin regions on their surfaces during cell differentiation. By simulating models where the excluded volume effects of closed chromatin regions are stronger than those of open chromatin regions, such rearrangement of open/closed chromatin regions on X-chromosome surfaces promoted the mutual approach of the Xic pair. These findings suggested that local intrachromosomal epigenetic features may contribute to the regulation of cell species-dependent differences in intranuclear architecture. The Biophysical Society of Japan 2022-05-10 /pmc/articles/PMC9174021/ /pubmed/35797402 http://dx.doi.org/10.2142/biophysico.bppb-v19.0018 Text en 2022 THE BIOPHYSICAL SOCIETY OF JAPAN https://creativecommons.org/licenses/by-nc-sa/4.0/This article is licensed under the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. To view a copy of this license, visit
https://creativecommons.org/licenses/by-nc-sa/4.0/. |
spellingShingle | Regular Article Komoto, Tetsushi Fujii, Masashi Awazu, Akinori Epigenetic-structural changes in X chromosomes promote Xic pairing during early differentiation of mouse embryonic stem cells |
title | Epigenetic-structural changes in X chromosomes promote Xic pairing during early differentiation of mouse embryonic stem cells |
title_full | Epigenetic-structural changes in X chromosomes promote Xic pairing during early differentiation of mouse embryonic stem cells |
title_fullStr | Epigenetic-structural changes in X chromosomes promote Xic pairing during early differentiation of mouse embryonic stem cells |
title_full_unstemmed | Epigenetic-structural changes in X chromosomes promote Xic pairing during early differentiation of mouse embryonic stem cells |
title_short | Epigenetic-structural changes in X chromosomes promote Xic pairing during early differentiation of mouse embryonic stem cells |
title_sort | epigenetic-structural changes in x chromosomes promote xic pairing during early differentiation of mouse embryonic stem cells |
topic | Regular Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9174021/ https://www.ncbi.nlm.nih.gov/pubmed/35797402 http://dx.doi.org/10.2142/biophysico.bppb-v19.0018 |
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