CRISPR-based systems for sensitive and rapid on-site COVID-19 diagnostics

The COVID-19 pandemic has strained healthcare systems. Sensitive, specific, and timely COVID-19 diagnosis is crucial for effective medical intervention and transmission control. RT-PCR is the most sensitive/specific, but requires costly equipment and trained personnel in centralized laboratories, wh...

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Detalles Bibliográficos
Autores principales: Soh, Jun Hui, Balleza, Enrique, Abdul Rahim, Muhammad Nadjad, Chan, Hsi-Min, Mohd Ali, Siswand, Chuah, Jacqueline Kai Chin, Edris, Sherif, Atef, Ahmed, Bahieldin, Ahmed, Ying, Jackie Y., Sabir, Jamal S.M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Ltd. 2022
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9174145/
https://www.ncbi.nlm.nih.gov/pubmed/35871983
http://dx.doi.org/10.1016/j.tibtech.2022.06.002
Descripción
Sumario:The COVID-19 pandemic has strained healthcare systems. Sensitive, specific, and timely COVID-19 diagnosis is crucial for effective medical intervention and transmission control. RT-PCR is the most sensitive/specific, but requires costly equipment and trained personnel in centralized laboratories, which are inaccessible to resource-limited areas. Antigen rapid tests enable point-of-care (POC) detection but are significantly less sensitive/specific. CRISPR-Cas systems are compatible with isothermal amplification and dipstick readout, enabling sensitive/specific on-site testing. However, improvements in sensitivity and workflow complexity are needed to spur clinical adoption. We outline the mechanisms/strategies of major CRISPR-Cas systems, evaluate their on-site diagnostic capabilities, and discuss future research directions.

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