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5-Azacytidine increases tanshinone production in Salvia miltiorrhiza hairy roots through epigenetic modulation

Recent studies have indicated strong connections between epigenetic modulation and secondary metabolites in plants. It is vital to understand the roles of epigenetics in the production of secondary metabolites. In this study, the inhibitor of DNA methylation 5-azacytidine (5-Az) was used on the hair...

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Autores principales: Yang, Bo-Cheng, Lee, Meng-Shiou, Lin, Ming-Kuem, Chang, Wen-Te
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9174287/
https://www.ncbi.nlm.nih.gov/pubmed/35672334
http://dx.doi.org/10.1038/s41598-022-12577-8
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author Yang, Bo-Cheng
Lee, Meng-Shiou
Lin, Ming-Kuem
Chang, Wen-Te
author_facet Yang, Bo-Cheng
Lee, Meng-Shiou
Lin, Ming-Kuem
Chang, Wen-Te
author_sort Yang, Bo-Cheng
collection PubMed
description Recent studies have indicated strong connections between epigenetic modulation and secondary metabolites in plants. It is vital to understand the roles of epigenetics in the production of secondary metabolites. In this study, the inhibitor of DNA methylation 5-azacytidine (5-Az) was used on the hairy roots of the medicinal plant Salvia miltiorrhiza to investigate its effect on secondary metabolite production, gene expression, methylation levels in genomic DNA and promoter regions. Our results showed that the contents of tanshinones in S. miltiorrhiza hairy roots increased by 1.5–5 times, and some genes in the biosynthesis pathway showed an upward trend. According to our NGS analysis, the methylation pattern in the promotor of the gene encoding copalyl diphosphate synthase (CPS) was altered, and 51 out of 145 cytosines were demethylated during 5-Az treatment. A total of 36 putative transcription factors (TFs) binding cites were identified in these demethylation sites. Among these TFs binding cites, cis-regulatory elements for the binding of NF-Y and MYB were frequently found in our results. This is the first report to demonstrate a possible mechanism of DNA methylation participating in tanshinone biosynthesis in S. miltiorrhiza hairy roots by modulating the CPS promoter and TFs binding sites.
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spelling pubmed-91742872022-06-09 5-Azacytidine increases tanshinone production in Salvia miltiorrhiza hairy roots through epigenetic modulation Yang, Bo-Cheng Lee, Meng-Shiou Lin, Ming-Kuem Chang, Wen-Te Sci Rep Article Recent studies have indicated strong connections between epigenetic modulation and secondary metabolites in plants. It is vital to understand the roles of epigenetics in the production of secondary metabolites. In this study, the inhibitor of DNA methylation 5-azacytidine (5-Az) was used on the hairy roots of the medicinal plant Salvia miltiorrhiza to investigate its effect on secondary metabolite production, gene expression, methylation levels in genomic DNA and promoter regions. Our results showed that the contents of tanshinones in S. miltiorrhiza hairy roots increased by 1.5–5 times, and some genes in the biosynthesis pathway showed an upward trend. According to our NGS analysis, the methylation pattern in the promotor of the gene encoding copalyl diphosphate synthase (CPS) was altered, and 51 out of 145 cytosines were demethylated during 5-Az treatment. A total of 36 putative transcription factors (TFs) binding cites were identified in these demethylation sites. Among these TFs binding cites, cis-regulatory elements for the binding of NF-Y and MYB were frequently found in our results. This is the first report to demonstrate a possible mechanism of DNA methylation participating in tanshinone biosynthesis in S. miltiorrhiza hairy roots by modulating the CPS promoter and TFs binding sites. Nature Publishing Group UK 2022-06-07 /pmc/articles/PMC9174287/ /pubmed/35672334 http://dx.doi.org/10.1038/s41598-022-12577-8 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Yang, Bo-Cheng
Lee, Meng-Shiou
Lin, Ming-Kuem
Chang, Wen-Te
5-Azacytidine increases tanshinone production in Salvia miltiorrhiza hairy roots through epigenetic modulation
title 5-Azacytidine increases tanshinone production in Salvia miltiorrhiza hairy roots through epigenetic modulation
title_full 5-Azacytidine increases tanshinone production in Salvia miltiorrhiza hairy roots through epigenetic modulation
title_fullStr 5-Azacytidine increases tanshinone production in Salvia miltiorrhiza hairy roots through epigenetic modulation
title_full_unstemmed 5-Azacytidine increases tanshinone production in Salvia miltiorrhiza hairy roots through epigenetic modulation
title_short 5-Azacytidine increases tanshinone production in Salvia miltiorrhiza hairy roots through epigenetic modulation
title_sort 5-azacytidine increases tanshinone production in salvia miltiorrhiza hairy roots through epigenetic modulation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9174287/
https://www.ncbi.nlm.nih.gov/pubmed/35672334
http://dx.doi.org/10.1038/s41598-022-12577-8
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