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Gene expression of the endocannabinoid system in endometrium through menstrual cycle
Endocannabinoids mediate cellular functions and their activity is controlled by a complex system of enzymes, membrane receptors and transport molecules. Endocannabinoids are present in endometrium, a cyclical regenerative tissue requiring tightly regulated cellular mechanisms for maturation. The obj...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9174470/ https://www.ncbi.nlm.nih.gov/pubmed/35672435 http://dx.doi.org/10.1038/s41598-022-13488-4 |
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author | Tanaka, Keisuke Amoako, Akwasi A. Mortlock, Sally Rogers, Peter A. W. Holdsworth-Carson, Sarah J. Donoghue, Jacqueline F. Teh, Wan Tinn Montgomery, Grant W. McKinnon, Brett |
author_facet | Tanaka, Keisuke Amoako, Akwasi A. Mortlock, Sally Rogers, Peter A. W. Holdsworth-Carson, Sarah J. Donoghue, Jacqueline F. Teh, Wan Tinn Montgomery, Grant W. McKinnon, Brett |
author_sort | Tanaka, Keisuke |
collection | PubMed |
description | Endocannabinoids mediate cellular functions and their activity is controlled by a complex system of enzymes, membrane receptors and transport molecules. Endocannabinoids are present in endometrium, a cyclical regenerative tissue requiring tightly regulated cellular mechanisms for maturation. The objective of this study was to investigate the gene expression of key elements involved in the endocannabinoid system across the menstrual cycle. RNA was isolated from endometrial tissue and genome-wide gene expression datasets were generated using RNA-sequencing. An a priori set of 70 genes associated with endocannabinoid system were selected from published literature. Gene expression across the menstrual cycle was analyzed using a moderated t test, corrected for multiple testing with Bonferroni’s method. A total of 40 of the 70 genes were present in > 90% of the samples, and significant differential gene expression identified for 29 genes. We identified 4 distinct regulation patterns for synthesizing enzymes, as well as a distinct regulation pattern for degradations and transporting enzymes. This study charts the expression of endometrial endocannabinoid system genes across the menstrual cycle. Altered expression of genes that control endocannabinoid may allow fine control over endocannabinoid concentrations and their influence on cellular function, maturation and differentiation as the endometrium matures through the menstrual cycle. |
format | Online Article Text |
id | pubmed-9174470 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-91744702022-06-09 Gene expression of the endocannabinoid system in endometrium through menstrual cycle Tanaka, Keisuke Amoako, Akwasi A. Mortlock, Sally Rogers, Peter A. W. Holdsworth-Carson, Sarah J. Donoghue, Jacqueline F. Teh, Wan Tinn Montgomery, Grant W. McKinnon, Brett Sci Rep Article Endocannabinoids mediate cellular functions and their activity is controlled by a complex system of enzymes, membrane receptors and transport molecules. Endocannabinoids are present in endometrium, a cyclical regenerative tissue requiring tightly regulated cellular mechanisms for maturation. The objective of this study was to investigate the gene expression of key elements involved in the endocannabinoid system across the menstrual cycle. RNA was isolated from endometrial tissue and genome-wide gene expression datasets were generated using RNA-sequencing. An a priori set of 70 genes associated with endocannabinoid system were selected from published literature. Gene expression across the menstrual cycle was analyzed using a moderated t test, corrected for multiple testing with Bonferroni’s method. A total of 40 of the 70 genes were present in > 90% of the samples, and significant differential gene expression identified for 29 genes. We identified 4 distinct regulation patterns for synthesizing enzymes, as well as a distinct regulation pattern for degradations and transporting enzymes. This study charts the expression of endometrial endocannabinoid system genes across the menstrual cycle. Altered expression of genes that control endocannabinoid may allow fine control over endocannabinoid concentrations and their influence on cellular function, maturation and differentiation as the endometrium matures through the menstrual cycle. Nature Publishing Group UK 2022-06-07 /pmc/articles/PMC9174470/ /pubmed/35672435 http://dx.doi.org/10.1038/s41598-022-13488-4 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Tanaka, Keisuke Amoako, Akwasi A. Mortlock, Sally Rogers, Peter A. W. Holdsworth-Carson, Sarah J. Donoghue, Jacqueline F. Teh, Wan Tinn Montgomery, Grant W. McKinnon, Brett Gene expression of the endocannabinoid system in endometrium through menstrual cycle |
title | Gene expression of the endocannabinoid system in endometrium through menstrual cycle |
title_full | Gene expression of the endocannabinoid system in endometrium through menstrual cycle |
title_fullStr | Gene expression of the endocannabinoid system in endometrium through menstrual cycle |
title_full_unstemmed | Gene expression of the endocannabinoid system in endometrium through menstrual cycle |
title_short | Gene expression of the endocannabinoid system in endometrium through menstrual cycle |
title_sort | gene expression of the endocannabinoid system in endometrium through menstrual cycle |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9174470/ https://www.ncbi.nlm.nih.gov/pubmed/35672435 http://dx.doi.org/10.1038/s41598-022-13488-4 |
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