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Taste Receptor Cells Generate Oscillating Receptor Potentials by Activating G Protein-Coupled Taste Receptors

The receptor potentials of taste receptor cells remain unclear. Here, we demonstrate that taste receptor cells generate oscillating depolarization (n = 7) with action potentials in response to sweet, bitter, umami, and salty taste substances. At a lower concentration of taste substances, taste recep...

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Autores principales: Nakao, Yoshiki, Tateno, Katsumi, Ohtubo, Yoshitaka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9174655/
https://www.ncbi.nlm.nih.gov/pubmed/35694396
http://dx.doi.org/10.3389/fphys.2022.883372
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author Nakao, Yoshiki
Tateno, Katsumi
Ohtubo, Yoshitaka
author_facet Nakao, Yoshiki
Tateno, Katsumi
Ohtubo, Yoshitaka
author_sort Nakao, Yoshiki
collection PubMed
description The receptor potentials of taste receptor cells remain unclear. Here, we demonstrate that taste receptor cells generate oscillating depolarization (n = 7) with action potentials in response to sweet, bitter, umami, and salty taste substances. At a lower concentration of taste substances, taste receptor cells exhibited oscillations in membrane potentials with a low frequency and small magnitude of depolarization. Although the respective waves contained no or 1–2 action potentials, the taste receptor cells generated action potentials continuously in the presence of taste stimuli. Both the frequency and magnitude of oscillations increased when the concentration was increased, to 0.67–1.43 Hz (n = 3) and Δ39–53 mV (n = 3) in magnitude from −64.7 ± 4.2 to −18.7 ± 5.9 mV, which may activate the ATP-permeable ion channels. In contrast, a sour tastant (10-mM HCl) induced membrane depolarization (Δ19.4 ± 9.5 mV, n = 4) with action potentials in type III taste receptor cells. Interestingly, NaCl (1 M) taste stimuli induced oscillation (n = 2) or depolarization (Δ10.5 ± 5.7 mV at the tonic component, n = 9). Our results indicate that the frequency and magnitude of oscillations increased with increasing taste substance concentrations. These parameters may contribute to the expression of taste “thickness.”
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spelling pubmed-91746552022-06-09 Taste Receptor Cells Generate Oscillating Receptor Potentials by Activating G Protein-Coupled Taste Receptors Nakao, Yoshiki Tateno, Katsumi Ohtubo, Yoshitaka Front Physiol Physiology The receptor potentials of taste receptor cells remain unclear. Here, we demonstrate that taste receptor cells generate oscillating depolarization (n = 7) with action potentials in response to sweet, bitter, umami, and salty taste substances. At a lower concentration of taste substances, taste receptor cells exhibited oscillations in membrane potentials with a low frequency and small magnitude of depolarization. Although the respective waves contained no or 1–2 action potentials, the taste receptor cells generated action potentials continuously in the presence of taste stimuli. Both the frequency and magnitude of oscillations increased when the concentration was increased, to 0.67–1.43 Hz (n = 3) and Δ39–53 mV (n = 3) in magnitude from −64.7 ± 4.2 to −18.7 ± 5.9 mV, which may activate the ATP-permeable ion channels. In contrast, a sour tastant (10-mM HCl) induced membrane depolarization (Δ19.4 ± 9.5 mV, n = 4) with action potentials in type III taste receptor cells. Interestingly, NaCl (1 M) taste stimuli induced oscillation (n = 2) or depolarization (Δ10.5 ± 5.7 mV at the tonic component, n = 9). Our results indicate that the frequency and magnitude of oscillations increased with increasing taste substance concentrations. These parameters may contribute to the expression of taste “thickness.” Frontiers Media S.A. 2022-05-25 /pmc/articles/PMC9174655/ /pubmed/35694396 http://dx.doi.org/10.3389/fphys.2022.883372 Text en Copyright © 2022 Nakao, Tateno and Ohtubo. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Nakao, Yoshiki
Tateno, Katsumi
Ohtubo, Yoshitaka
Taste Receptor Cells Generate Oscillating Receptor Potentials by Activating G Protein-Coupled Taste Receptors
title Taste Receptor Cells Generate Oscillating Receptor Potentials by Activating G Protein-Coupled Taste Receptors
title_full Taste Receptor Cells Generate Oscillating Receptor Potentials by Activating G Protein-Coupled Taste Receptors
title_fullStr Taste Receptor Cells Generate Oscillating Receptor Potentials by Activating G Protein-Coupled Taste Receptors
title_full_unstemmed Taste Receptor Cells Generate Oscillating Receptor Potentials by Activating G Protein-Coupled Taste Receptors
title_short Taste Receptor Cells Generate Oscillating Receptor Potentials by Activating G Protein-Coupled Taste Receptors
title_sort taste receptor cells generate oscillating receptor potentials by activating g protein-coupled taste receptors
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9174655/
https://www.ncbi.nlm.nih.gov/pubmed/35694396
http://dx.doi.org/10.3389/fphys.2022.883372
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