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Gene Expression Profiles of the Aging Rat Hippocampus Imply Altered Immunoglobulin Dynamics

Aging is a process that leads to the deterioration in physiological functioning of the brain. Prior research has proposed that hippocampal aging is accompanied by genetic alterations in neural, synaptic, and immune functions. Nevertheless, interactome-based interrogations of gene alterations in hipp...

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Autores principales: Giannos, Panagiotis, Prokopidis, Konstantinos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9174800/
https://www.ncbi.nlm.nih.gov/pubmed/35692421
http://dx.doi.org/10.3389/fnins.2022.915907
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author Giannos, Panagiotis
Prokopidis, Konstantinos
author_facet Giannos, Panagiotis
Prokopidis, Konstantinos
author_sort Giannos, Panagiotis
collection PubMed
description Aging is a process that leads to the deterioration in physiological functioning of the brain. Prior research has proposed that hippocampal aging is accompanied by genetic alterations in neural, synaptic, and immune functions. Nevertheless, interactome-based interrogations of gene alterations in hippocampal aging, remain scarce. Our study integrated gene expression profiles of the hippocampus from young and aged rats and functionally classified network-mapped genes based on their interactome. Hippocampal differentially expressed genes (DEGs) between young (5–8 months) and aged (21–26 months) male rats (Rattus norvegicus) were retrieved from five publicly available datasets (GSE14505, GSE20219, GSE14723, GSE14724, and GSE14725; 38 young and 29 aged samples). Encoded hippocampal proteins of age-related DEGs and their interactome were predicted. Clustered network DEGs were identified and the highest-ranked was functionally annotated. A single cluster of 19 age-related hippocampal DEGs was revealed, which was linked with immune response (biological process, P = 1.71E-17), immunoglobulin G binding (molecular function, P = 1.92E-08), and intrinsic component of plasma membrane (cellular component, P = 1.25E-06). Our findings revealed dysregulated hippocampal immunoglobulin dynamics in the aging rat brain. Whether a consequence of neurovascular perturbations and dysregulated blood-brain barrier permeability, the role of hippocampal immunoregulation in the pathobiology of aging warrants further investigation.
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spelling pubmed-91748002022-06-09 Gene Expression Profiles of the Aging Rat Hippocampus Imply Altered Immunoglobulin Dynamics Giannos, Panagiotis Prokopidis, Konstantinos Front Neurosci Neuroscience Aging is a process that leads to the deterioration in physiological functioning of the brain. Prior research has proposed that hippocampal aging is accompanied by genetic alterations in neural, synaptic, and immune functions. Nevertheless, interactome-based interrogations of gene alterations in hippocampal aging, remain scarce. Our study integrated gene expression profiles of the hippocampus from young and aged rats and functionally classified network-mapped genes based on their interactome. Hippocampal differentially expressed genes (DEGs) between young (5–8 months) and aged (21–26 months) male rats (Rattus norvegicus) were retrieved from five publicly available datasets (GSE14505, GSE20219, GSE14723, GSE14724, and GSE14725; 38 young and 29 aged samples). Encoded hippocampal proteins of age-related DEGs and their interactome were predicted. Clustered network DEGs were identified and the highest-ranked was functionally annotated. A single cluster of 19 age-related hippocampal DEGs was revealed, which was linked with immune response (biological process, P = 1.71E-17), immunoglobulin G binding (molecular function, P = 1.92E-08), and intrinsic component of plasma membrane (cellular component, P = 1.25E-06). Our findings revealed dysregulated hippocampal immunoglobulin dynamics in the aging rat brain. Whether a consequence of neurovascular perturbations and dysregulated blood-brain barrier permeability, the role of hippocampal immunoregulation in the pathobiology of aging warrants further investigation. Frontiers Media S.A. 2022-05-25 /pmc/articles/PMC9174800/ /pubmed/35692421 http://dx.doi.org/10.3389/fnins.2022.915907 Text en Copyright © 2022 Giannos and Prokopidis. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Giannos, Panagiotis
Prokopidis, Konstantinos
Gene Expression Profiles of the Aging Rat Hippocampus Imply Altered Immunoglobulin Dynamics
title Gene Expression Profiles of the Aging Rat Hippocampus Imply Altered Immunoglobulin Dynamics
title_full Gene Expression Profiles of the Aging Rat Hippocampus Imply Altered Immunoglobulin Dynamics
title_fullStr Gene Expression Profiles of the Aging Rat Hippocampus Imply Altered Immunoglobulin Dynamics
title_full_unstemmed Gene Expression Profiles of the Aging Rat Hippocampus Imply Altered Immunoglobulin Dynamics
title_short Gene Expression Profiles of the Aging Rat Hippocampus Imply Altered Immunoglobulin Dynamics
title_sort gene expression profiles of the aging rat hippocampus imply altered immunoglobulin dynamics
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9174800/
https://www.ncbi.nlm.nih.gov/pubmed/35692421
http://dx.doi.org/10.3389/fnins.2022.915907
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