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Novel Single Nucleotide Polymorphisms (SNPs) and Genetic Features of the Prion Protein Gene (PRNP) in Quail (Coturnix japonica)

Prion diseases are fatal infectious diseases caused by conformational changes of a prion protein (PrP(Sc)) derived from a normal prion protein (PrP(C)). Prion diseases have been reported in several mammalian hosts but not in any birds, including the most popular poultry species, of which chickens sh...

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Autores principales: Kim, Yoonhee, Kim, Yong-Chan, Jeong, Byung-Hoon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9174905/
https://www.ncbi.nlm.nih.gov/pubmed/35692300
http://dx.doi.org/10.3389/fvets.2022.870735
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author Kim, Yoonhee
Kim, Yong-Chan
Jeong, Byung-Hoon
author_facet Kim, Yoonhee
Kim, Yong-Chan
Jeong, Byung-Hoon
author_sort Kim, Yoonhee
collection PubMed
description Prion diseases are fatal infectious diseases caused by conformational changes of a prion protein (PrP(Sc)) derived from a normal prion protein (PrP(C)). Prion diseases have been reported in several mammalian hosts but not in any birds, including the most popular poultry species, of which chickens showed some resistance to experimental prion infection. To identify the genetic polymorphisms in the quail prion protein gene (PRNP), polymerase chain reaction and DNA sequencing were performed with gene-specific primers in 164 quails. Four in silico programs, including PROVEAN, PANTHER, SIFT, and AMYCO, were used to investigate the effect of non-synonymous single nucleotide polymorphisms (SNPs) on quail PrP. Furthermore, to investigate the genetic relationship of avian PrPs, phylogenetic analysis and multiple sequence alignments were performed using MEGA X program. Finally, the secondary and tertiary structures of avian PrPs were analyzed by SWISS-MODEL. We identified 33 novel SNPs in the quail PRNP gene, including three non-synonymous SNPs, c.56C>T (T19I), c.60C>T (V21I), and c.61G>A (A22S). Although V21I was predicted to have deleterious effects by SIFT, the substitutions of all three amino acids did not affect the amyloid propensity, 3D structure, or hydrogen bonds of quail PrP. Quail PrP showed a close evolutionary relationship and similar secondary and tertiary structures to chicken PrP compared to duck PrP. To our knowledge, this is the first report on the genetic and structural properties of the quail PRNP gene.
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spelling pubmed-91749052022-06-09 Novel Single Nucleotide Polymorphisms (SNPs) and Genetic Features of the Prion Protein Gene (PRNP) in Quail (Coturnix japonica) Kim, Yoonhee Kim, Yong-Chan Jeong, Byung-Hoon Front Vet Sci Veterinary Science Prion diseases are fatal infectious diseases caused by conformational changes of a prion protein (PrP(Sc)) derived from a normal prion protein (PrP(C)). Prion diseases have been reported in several mammalian hosts but not in any birds, including the most popular poultry species, of which chickens showed some resistance to experimental prion infection. To identify the genetic polymorphisms in the quail prion protein gene (PRNP), polymerase chain reaction and DNA sequencing were performed with gene-specific primers in 164 quails. Four in silico programs, including PROVEAN, PANTHER, SIFT, and AMYCO, were used to investigate the effect of non-synonymous single nucleotide polymorphisms (SNPs) on quail PrP. Furthermore, to investigate the genetic relationship of avian PrPs, phylogenetic analysis and multiple sequence alignments were performed using MEGA X program. Finally, the secondary and tertiary structures of avian PrPs were analyzed by SWISS-MODEL. We identified 33 novel SNPs in the quail PRNP gene, including three non-synonymous SNPs, c.56C>T (T19I), c.60C>T (V21I), and c.61G>A (A22S). Although V21I was predicted to have deleterious effects by SIFT, the substitutions of all three amino acids did not affect the amyloid propensity, 3D structure, or hydrogen bonds of quail PrP. Quail PrP showed a close evolutionary relationship and similar secondary and tertiary structures to chicken PrP compared to duck PrP. To our knowledge, this is the first report on the genetic and structural properties of the quail PRNP gene. Frontiers Media S.A. 2022-05-25 /pmc/articles/PMC9174905/ /pubmed/35692300 http://dx.doi.org/10.3389/fvets.2022.870735 Text en Copyright © 2022 Kim, Kim and Jeong. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Kim, Yoonhee
Kim, Yong-Chan
Jeong, Byung-Hoon
Novel Single Nucleotide Polymorphisms (SNPs) and Genetic Features of the Prion Protein Gene (PRNP) in Quail (Coturnix japonica)
title Novel Single Nucleotide Polymorphisms (SNPs) and Genetic Features of the Prion Protein Gene (PRNP) in Quail (Coturnix japonica)
title_full Novel Single Nucleotide Polymorphisms (SNPs) and Genetic Features of the Prion Protein Gene (PRNP) in Quail (Coturnix japonica)
title_fullStr Novel Single Nucleotide Polymorphisms (SNPs) and Genetic Features of the Prion Protein Gene (PRNP) in Quail (Coturnix japonica)
title_full_unstemmed Novel Single Nucleotide Polymorphisms (SNPs) and Genetic Features of the Prion Protein Gene (PRNP) in Quail (Coturnix japonica)
title_short Novel Single Nucleotide Polymorphisms (SNPs) and Genetic Features of the Prion Protein Gene (PRNP) in Quail (Coturnix japonica)
title_sort novel single nucleotide polymorphisms (snps) and genetic features of the prion protein gene (prnp) in quail (coturnix japonica)
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9174905/
https://www.ncbi.nlm.nih.gov/pubmed/35692300
http://dx.doi.org/10.3389/fvets.2022.870735
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