Cargando…

Multi-Omics Analysis of MCM2 as a Promising Biomarker in Pan-Cancer

Minichromosome maintenance 2 (MCM2) is a member of the minichromosomal maintenance family of proteins that mainly regulates DNA replication and the cell cycle and is involved in regulating cancer cell proliferation in various cancers. Previous studies have reported that MCM2 plays a pivotal role in...

Descripción completa

Detalles Bibliográficos
Autores principales: Yuan, Jing, Lan, Hua, Huang, Dongqing, Guo, Xiaohui, Liu, Chu, Liu, Shuping, Zhang, Peng, Cheng, Yan, Xiao, Songshu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9174984/
https://www.ncbi.nlm.nih.gov/pubmed/35693940
http://dx.doi.org/10.3389/fcell.2022.852135
_version_ 1784722355321307136
author Yuan, Jing
Lan, Hua
Huang, Dongqing
Guo, Xiaohui
Liu, Chu
Liu, Shuping
Zhang, Peng
Cheng, Yan
Xiao, Songshu
author_facet Yuan, Jing
Lan, Hua
Huang, Dongqing
Guo, Xiaohui
Liu, Chu
Liu, Shuping
Zhang, Peng
Cheng, Yan
Xiao, Songshu
author_sort Yuan, Jing
collection PubMed
description Minichromosome maintenance 2 (MCM2) is a member of the minichromosomal maintenance family of proteins that mainly regulates DNA replication and the cell cycle and is involved in regulating cancer cell proliferation in various cancers. Previous studies have reported that MCM2 plays a pivotal role in cell proliferation and cancer development. However, few articles have systematically reported the pathogenic roles of MCM2 across cancers. Therefore, the present pan-cancer study was conducted. Various computational tools were used to investigate the MCM2 expression level, genetic mutation rate, and regulating mechanism, immune infiltration, tumor diagnosis and prognosis, therapeutic response and drug sensitivity of various cancers. The expression and function of MCM2 were examined by Western blotting and CCK-8 assays. MCM2 was significantly upregulated in almost all cancers and cancer subtypes in The Cancer Genome Atlas and was closely associated with tumor mutation burden, tumor stage, and immune therapy response. Upregulation of MCM2 expression may be correlated with a high level of alterations rate. MCM2 expression was associated with the infiltration of various immune cells and molecules and markedly associated with a poor prognosis. Western blotting and CCK-8 assays revealed that MCM2 expression was significantly upregulated in melanoma cell lines. Our results also suggested that MCM2 promotes cell proliferation in vitro by activating cell proliferation pathways such as the Akt signaling pathways. This study explored the oncogenic role of MCM2 across cancers, provided data on the underlying mechanisms of these cancers for further research and demonstrated that MCM2 may be a promising target for cancer immunotherapy.
format Online
Article
Text
id pubmed-9174984
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-91749842022-06-09 Multi-Omics Analysis of MCM2 as a Promising Biomarker in Pan-Cancer Yuan, Jing Lan, Hua Huang, Dongqing Guo, Xiaohui Liu, Chu Liu, Shuping Zhang, Peng Cheng, Yan Xiao, Songshu Front Cell Dev Biol Cell and Developmental Biology Minichromosome maintenance 2 (MCM2) is a member of the minichromosomal maintenance family of proteins that mainly regulates DNA replication and the cell cycle and is involved in regulating cancer cell proliferation in various cancers. Previous studies have reported that MCM2 plays a pivotal role in cell proliferation and cancer development. However, few articles have systematically reported the pathogenic roles of MCM2 across cancers. Therefore, the present pan-cancer study was conducted. Various computational tools were used to investigate the MCM2 expression level, genetic mutation rate, and regulating mechanism, immune infiltration, tumor diagnosis and prognosis, therapeutic response and drug sensitivity of various cancers. The expression and function of MCM2 were examined by Western blotting and CCK-8 assays. MCM2 was significantly upregulated in almost all cancers and cancer subtypes in The Cancer Genome Atlas and was closely associated with tumor mutation burden, tumor stage, and immune therapy response. Upregulation of MCM2 expression may be correlated with a high level of alterations rate. MCM2 expression was associated with the infiltration of various immune cells and molecules and markedly associated with a poor prognosis. Western blotting and CCK-8 assays revealed that MCM2 expression was significantly upregulated in melanoma cell lines. Our results also suggested that MCM2 promotes cell proliferation in vitro by activating cell proliferation pathways such as the Akt signaling pathways. This study explored the oncogenic role of MCM2 across cancers, provided data on the underlying mechanisms of these cancers for further research and demonstrated that MCM2 may be a promising target for cancer immunotherapy. Frontiers Media S.A. 2022-05-25 /pmc/articles/PMC9174984/ /pubmed/35693940 http://dx.doi.org/10.3389/fcell.2022.852135 Text en Copyright © 2022 Yuan, Lan, Huang, Guo, Liu, Liu, Zhang, Cheng and Xiao. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Yuan, Jing
Lan, Hua
Huang, Dongqing
Guo, Xiaohui
Liu, Chu
Liu, Shuping
Zhang, Peng
Cheng, Yan
Xiao, Songshu
Multi-Omics Analysis of MCM2 as a Promising Biomarker in Pan-Cancer
title Multi-Omics Analysis of MCM2 as a Promising Biomarker in Pan-Cancer
title_full Multi-Omics Analysis of MCM2 as a Promising Biomarker in Pan-Cancer
title_fullStr Multi-Omics Analysis of MCM2 as a Promising Biomarker in Pan-Cancer
title_full_unstemmed Multi-Omics Analysis of MCM2 as a Promising Biomarker in Pan-Cancer
title_short Multi-Omics Analysis of MCM2 as a Promising Biomarker in Pan-Cancer
title_sort multi-omics analysis of mcm2 as a promising biomarker in pan-cancer
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9174984/
https://www.ncbi.nlm.nih.gov/pubmed/35693940
http://dx.doi.org/10.3389/fcell.2022.852135
work_keys_str_mv AT yuanjing multiomicsanalysisofmcm2asapromisingbiomarkerinpancancer
AT lanhua multiomicsanalysisofmcm2asapromisingbiomarkerinpancancer
AT huangdongqing multiomicsanalysisofmcm2asapromisingbiomarkerinpancancer
AT guoxiaohui multiomicsanalysisofmcm2asapromisingbiomarkerinpancancer
AT liuchu multiomicsanalysisofmcm2asapromisingbiomarkerinpancancer
AT liushuping multiomicsanalysisofmcm2asapromisingbiomarkerinpancancer
AT zhangpeng multiomicsanalysisofmcm2asapromisingbiomarkerinpancancer
AT chengyan multiomicsanalysisofmcm2asapromisingbiomarkerinpancancer
AT xiaosongshu multiomicsanalysisofmcm2asapromisingbiomarkerinpancancer